The mouse genome at oxford: What can mouse gene mapping do for mammalian genetics?

BioEssays ◽  
1989 ◽  
Vol 11 (6) ◽  
pp. 191-193 ◽  
Author(s):  
S. D. M. Brown
Keyword(s):  
Gene Mapping ◽  
Mouse Genome ◽  
Mouse Gene ◽  
Mammalian Genetics

A set of anonymous DNA clones as markers for mouse gene mapping

1992 ◽  
Vol 3 (4) ◽  
pp. 244-246 ◽  
Author(s):  
Henri Le Roy ◽  
Dominique Simon-Chazottes ◽  
Xavier Montagutelli ◽  
Jean-Louis Guénet
Keyword(s):  
Gene Mapping ◽  
Mouse Gene

Mouse gene trap approach: identification of novel genes and characterization of their biological functions

1998 ◽  
Vol 76 (6) ◽  
pp. 1029-1037 ◽  
Author(s):  
Kenji Kitajima ◽  
Takashi Takeuchi
Keyword(s):  
Insertional Mutagenesis ◽  
Mouse Genome ◽  
Gene Trap ◽  
Mouse Gene ◽  
Selection Marker ◽  
Biological Functions ◽  
Exogenous Dna ◽  
Biological Phenomena ◽  
Endogenous Genes

The mouse gene trap strategy is an insertional mutagenesis involving an exogenous DNA, termed the trap vector, as a mutagen that produces a mutation in the mouse genome and a sequence tag to facilitate the isolation of the mutated genes. The trap vector consists of a reporter gene whose expression mimics that of the endogenous genes mutated and a selection marker that sorts cells bearing the inserted vector. Gene trap is a powerful method for identifying genes important in biological phenomena. Moreover, the method produces mutant organisms whose phenotypes provide invaluable information about the biological functions of the genes responsible for these phenotypes. Indeed, a number of genes essential for mouse embryogenesis have been identified by the gene trap method. Here, we describe the principle, results, and perspectives for applications of gene trap approach to the study of cell differentiation and lineage commitment.Key words: gene trap, embryogenesis, jumonji.

A somatic cell hybrid panel for mouse gene mapping characterized by PCR and FISH

1995 ◽  
Vol 6 (6) ◽  
pp. 429-432 ◽  
Author(s):  
P. Williamson ◽  
S. Holt ◽  
S. Townsend ◽  
Y. Boyd
Keyword(s):  
Gene Mapping ◽  
Somatic Cell ◽  
Cell Hybrid ◽  
Somatic Cell Hybrid ◽  
Mouse Gene ◽  
Somatic Cell Hybrid Panel

scholarly journals Identification and applications of repetitive probes for gene mapping in the mouse.

Genetics ◽  
1991 ◽  
Vol 127 (1) ◽  
pp. 169-179
Author(s):  
L D Siracusa ◽  
N A Jenkins ◽  
N G Copeland
Keyword(s):  
Genetic Variation ◽  
Gene Mapping ◽  
Mouse Genome ◽  
Interspecific Cross ◽  
Single Copy ◽  
Molecular Probes ◽  
Envelope Gene ◽  
Neoplastic Disease ◽  
Virus Envelope ◽  
Tumor Virus

Abstract Interspecific mouse hybrids that are viable and fertile provide a wealth of genetic variation that is useful for gene mapping. We are using this genetic variation to develop multilocus linkage maps of the mouse genome. As an outgrowth of this work, we have identified three repetitive probes that collectively identify 28 loci dispersed on 16 of the 19 mouse autosomes and the X chromosome. These loci establish a skeleton linkage map that can be used to detect linkage over much of the mouse genome. The molecular probes are derived from the mouse mammary tumor virus envelope gene, the ornithine decarboxylase gene, and the triose phosphate isomerase gene. The ability to scan the mouse genome quickly and efficiently in an interspecific cross using these three repetitive probes makes this system a powerful tool for identifying the chromosomal location of mutations that have yet to be cloned, mapping multigenic traits, and identifying recessive protooncogene loci associated with murine neoplastic disease. Ultimately, interspecific hybrids in conjunction with repetitive and single-copy probes will provide a rapid means to access virtually any gene of interest in the mouse genome at the molecular level.

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

Diagnosls of α thalassemia in the newborn. cord blood survey utilizing gene mapping

Pathology ◽  
1984 ◽  
Vol 16 (1) ◽  
pp. 16-21 ◽  
Author(s):  
Ronald J. Trent ◽  
P.E. Brock ◽  
J. Yakas ◽  
L.M. Trent ◽  
H. Kronenberg
Keyword(s):  
Gene Mapping ◽  
Cord Blood
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