Electrophoretic karyotype of the zygomycete Absidia glauca: evidence for differences between mating types

1991 ◽  
Vol 19 (4) ◽  
pp. 279-284 ◽  
Author(s):  
Torsten Kayser ◽  
Johannes W�stemeyer
Keyword(s):  
Electrophoretic Karyotype ◽  
Mating Types ◽  
Absidia Glauca

Complementary mating types of Mucor circinelloides show electrophoretic karyotype heterogeneity

1999 ◽  
Vol 36 (6) ◽  
pp. 383-389 ◽  
Author(s):  
José María Díaz-Mínguez ◽  
M. Angeles López-Matas ◽  
Arturo P. Eslava
Keyword(s):  
Mucor Circinelloides ◽  
Electrophoretic Karyotype ◽  
Mating Types ◽  
Karyotype Heterogeneity

Relationships between sexual processes and parasitic interactions in the host–pathogen systemAbsidia glauca–Parasitella parasitica

1995 ◽  
Vol 73 (S1) ◽  
pp. 243-250 ◽  
Author(s):  
J. Wöstemeyer ◽  
A. Wöstemeyer ◽  
A. Burmester ◽  
K. Czempinski
Keyword(s):  
Mating Type ◽  
Molecular Level ◽  
Acid Synthesis ◽  
Infection Process ◽  
Mating Types ◽  
Infection Structures ◽  
Absidia Glauca ◽  
Facultative Parasite ◽  
Parasitic Interactions ◽  
Sexual Processes

Parasitella parasitica is a facultative parasite of many Mucorales including Absidia glauca. The infection process includes the formation of a plasmatic continuum between host and parasite, which allows the invasion of the host by nuclei of the parasite. This process gives rise to interspecies recombinants. Auxotrophic A. glauca mutants are complemented by the transfer of genes from the parasite to the host. At the molecular level, we could show that plasmid-coded genes are also transferred. The successful formation of infection structures is mating-type dependent. Parasitella parasitica exclusively infects hosts belonging to the complementary mating type. The formation of infection structures is correlated with the ability to synthesise the sex pheromone trisporic acid, which is produced in mixed cultures by compatible combinations between host and parasite. Normally, trisporic acid is formed by a cooperative biosynthesis involving both mating types. Trisporic acid seems to be involved also in mediating the recognition between P. parasitica and A. glauca. To test the hypothesis of direct hormonal correlations between sex and parasitism, we have isolated one of the key enzymes for trisporic acid synthesis, dihydromethyltrisporic acid dehydrogenase, sequenced the N-termini of some proteolytic cleavage products, and have started to isolate the corresponding gene. Key words: mycoparasitism, zygomycetes, trisporic acid, Absidia glauca, Parasitella parasitica, Mucor.

Dimorphism of rDNA organization in mating types of the zygomycete Absidia glauca

1994 ◽  
Vol 149 (4) ◽  
pp. 407-411 ◽  
Author(s):  
Johannes Wöstemeyer ◽  
Anke Burmester
Keyword(s):  
Mating Types ◽  
Absidia Glauca ◽  
Rdna Organization

scholarly journals Differential Gene Expression between Fungal Mating Types Is Associated with Sequence Degeneration

2020 ◽  
Vol 12 (4) ◽  
pp. 243-258 ◽  
Author(s):  
Wen-Juan Ma ◽  
Fantin Carpentier ◽  
Tatiana Giraud ◽  
Michael E Hood
Keyword(s):  
Differential Expression ◽  
Differentially Expressed Genes ◽  
Mating Type ◽  
Sex Chromosomes ◽  
Sex Chromosome ◽  
Gc Content ◽  
Differentially Expressed ◽  
Mating Types ◽  
Sexual Antagonism ◽  
Recombination Suppression

Abstract Degenerative mutations in non-recombining regions, such as in sex chromosomes, may lead to differential expression between alleles if mutations occur stochastically in one or the other allele. Reduced allelic expression due to degeneration has indeed been suggested to occur in various sex-chromosome systems. However, whether an association occurs between specific signatures of degeneration and differential expression between alleles has not been extensively tested, and sexual antagonism can also cause differential expression on sex chromosomes. The anther-smut fungus Microbotryum lychnidis-dioicae is ideal for testing associations between specific degenerative signatures and differential expression because 1) there are multiple evolutionary strata on the mating-type chromosomes, reflecting successive recombination suppression linked to mating-type loci; 2) separate haploid cultures of opposite mating types help identify differential expression between alleles; and 3) there is no sexual antagonism as a confounding factor accounting for differential expression. We found that differentially expressed genes were enriched in the four oldest evolutionary strata compared with other genomic compartments, and that, within compartments, several signatures of sequence degeneration were greater for differentially expressed than non-differentially expressed genes. Two particular degenerative signatures were significantly associated with lower expression levels within differentially expressed allele pairs: upstream insertion of transposable elements and mutations truncating the protein length. Other degenerative mutations associated with differential expression included nonsynonymous substitutions and altered intron or GC content. The association between differential expression and allele degeneration is relevant for a broad range of taxa where mating compatibility or sex is determined by genes located in large regions where recombination is suppressed.

scholarly journals Deletion of the Schizophyllum commune Aα Locus: The Roles of Aα Y and Z Mating-Type Genes

Genetics ◽  
1996 ◽  
Vol 144 (4) ◽  
pp. 1437-1444
Author(s):  
C Ian Robertson ◽  
Kirk A Bartholomew ◽  
Charles P Novotny ◽  
Robert C Ullrich
Keyword(s):  
Mating Type ◽  
Sexual Development ◽  
Schizophyllum Commune ◽  
Mating Types ◽  
Developmental Pathway ◽  
Mating Type Gene ◽  
Mating Type Genes ◽  
Regulatory Loci ◽  
Type Gene

The Aα locus is one of four master regulatory loci that determine mating type and regulate sexual development in Schizophyllum commune. We have made a plasmid containing a URA1 gene disruption of the Aα Y1 gene. Y1 is the sole Aα gene in Aα1 strains. We used the plasmid construction to produce an Aα null (i.e., AαΔ) strain by replacing the genomic Y1 gene with URA1 in an Aα1 strain. To characterize the role of the Aα genes in the regulation of sexual development, we transformed various Aα Y and Z alleles into AαΔ strains and examined the acquired mating types and mating abilities of the transformants. These experiments demonstrate that the Aα Y gene is not essential for fungal viability and growth, that a solitary Z Aα mating-type gene does not itself activate development, that Aβ proteins are sufficient to activate the A developmental pathway in the absence of Aα proteins and confirm that Y and Z genes are the sole determinants of Aα mating type. The data from these experiments support and refine our model of the regulation of A-pathway events by Y and Z proteins.

New Mating Types in Physarum polycephalum

Mycologia ◽  
1977 ◽  
Vol 69 (2) ◽  
pp. 421 ◽  
Author(s):  
O'Neil Ray Collins ◽  
Hsi-Chang Tang
Keyword(s):  
Physarum Polycephalum ◽  
Mating Types

scholarly journals A PCR-based Assay for Distinguishing between A1 and A2 Mating Types of Phytophthora capsici

2017 ◽  
Vol 142 (4) ◽  
pp. 260-264
Author(s):  
Ping Li ◽  
Dong Liu ◽  
Min Guo ◽  
Yuemin Pan ◽  
Fangxin Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mating Type ◽  
Phytophthora Capsici ◽  
Mating Types ◽  
Sequence Repeat ◽  
Chain Reaction ◽  
Plant Parasite ◽  
Dna Fragment ◽  
Polymerase Chain ◽  
Microsatellite Diversity

Sexual reproduction in the plant parasite Phytophthora capsici Leonian requires the interaction of two distinct mating types, A1 and A2. Co-occurrence of these mating types can enhance the genetic diversity of P. capsici and alter its virulence or resistance characteristics. Using an intersimple sequence repeat (ISSR) screen of microsatellite diversity, we identified, cloned, and sequenced a novel 1121-base pair (bp) fragment specific to the A1 mating type of P. capsici. Primers Pcap-1 and Pcap-2 were designed from this DNA fragment to specifically detect the A1 mating type. Polymerase chain reaction (PCR) using these primers amplified an expected 997-bp fragment from known A1 mating types, but yielded a 508-bp fragment from known A2 mating types. This PCR-based assay could be adapted to accurately and rapidly detect the co-occurrence of A1 and A2 P. capsici mating types from field material.

Distribution of mating types and genetic diversity induced by sexual recombination in Setosphaeria turcica in northern China

2007 ◽  
Vol 1 (4) ◽  
pp. 368-376 ◽  
Author(s):  
Yongshan Fan ◽  
Jifang Ma ◽  
Xiumei Gui ◽  
Xinlong An ◽  
Shuqin Sun ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Northern China ◽  
Mating Types ◽  
Setosphaeria Turcica ◽  
Sexual Recombination
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