Genome-size variation in bivalve molluscs determined by flow cytometry

1996 ◽  
Vol 126 (3) ◽  
pp. 489-497 ◽  
Author(s):  
A. M. Rodr�guez-Ju�z ◽  
M. Torrado ◽  
J. M�ndez
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Size Variation ◽  
Bivalve Molluscs ◽  
Genome Size Variation

The genome sizes of Hordeum species show considerable variation

Genome ◽  
1996 ◽  
Vol 39 (4) ◽  
pp. 730-735 ◽  
Author(s):  
Juha Kankanpää ◽  
Alan H. Schulman ◽  
Leena Mannonen
Keyword(s):  
Flow Cytometry ◽  
Hordeum Vulgare ◽  
Genome Size ◽  
Nuclear Dna ◽  
Size Variation ◽  
Nuclear Dna Content ◽  
Meiotic Pairing ◽  
Genome Size Variation ◽  
Dapi Staining ◽  
Hordeum Vulgare Ssp

Hordeum, distributed worldwide in temperate zones, is the second largest genus in the tribe Triticeae and includes diploid, tetraploid, and hexaploid species. We determined, by DAPI staining and flow cytometry, the nuclear DNA content for 35 accessions of the genus Hordeum, from a total of 19 species, including specimens of 2 cultivars and 2 landraces of Hordeum vulgare ssp. vulgare as well as samples of 12 Hordeum vulgare ssp. spontaneum populations. Genome sizes ranged from 5.69 to 9.41 pg for the G1 nuclei of the diploids, and from 13.13 to 18.36 pg for those of the tetraploids. This constitutes a 1.7-fold variation for the diploids, contrasting with a 4% variation previously reported. For H. vulgare ssp. vulgare (barley), the accessions examined differed by 18%. These variations in genome size cannot be correlated with meiotic pairing groups (I, H, X, Y) or with proposed phylogenetic relationships within the genus. Genome size variation between barley accessions cannot be related to status as cultivated or wild, or to climatic or geological gradients. We suggest these data may indicate rapid but sporadic changes in genome size within the genus. Key words : barley, Hordeum, Triticeae, genome size, flow cytometry.

scholarly journals Genome size variation and karyotype diversity in eight taxa of Sorbus sensu stricto (Rosaceae) from China

2021 ◽  
Vol 15 (2) ◽  
pp. 137-148
Author(s):  
Jiabao Li ◽  
Kailin Zhu ◽  
Qin Wang ◽  
Xin Chen
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Size Variation ◽  
Sensu Stricto ◽  
Chromosome Size ◽  
Chromosome Counting ◽  
Genome Size Variation ◽  
Ploidy Levels ◽  
First Time ◽  
Karyotype Symmetry

Eight taxa of Sorbus Linnaeus, 1753 sensu stricto (Rosaceae) from China have been studied karyologically through chromosome counting, chromosomal measurement and karyotype symmetry. Genome size was also estimated by flow cytometry. Six taxa, S. amabilis Cheng ex T.T.Yu et K.C.Kuan, 1963, S. hupehensis var. paucijuga (D.K. Zang et P.C. Huang, 1992) L.T. Lu, 2000, S. koehneana C.K. Schneider, 1906, S. pohuashanensis (Hance, 1875) Hedlund, 1901, S. scalaris Koehne, 1913 and S. wilsoniana C.K. Schneider, 1906 are diploids with 2n = 34, whereas two taxa, S. filipes Handel-Mazzetti,1933 and S. ovalis McAllister, 2005 are tetraploid with 2n = 68. In general, the chromosome size is mainly small, and karyotypes are symmetrical with predominance of metacentric chromosomes. Genome size variation of diploids and tetraploids is 1.401 pg –1.676 pg and 2.674 pg –2.684 pg, respectively. Chromosome numbers of S. amabilis and S. hupehensis var. paucijuga, and karyotype and genome size of eight taxa studied are reported for the first time. This study emphasised the reliability of flow cytometry in genome size determination to infer ploidy levels in Chinese native Sorbus species.

scholarly journals Genome size variation in the pine fusiform rust pathogenCronartium quercuumf.sp.fusiformeas determined by flow cytometry

Mycologia ◽  
2010 ◽  
Vol 102 (6) ◽  
pp. 1295-1302 ◽  
Author(s):  
Claire L. Anderson ◽  
Thomas L. Kubisiak ◽  
C. Dana Nelson ◽  
Jason A. Smith ◽  
John M. Davis
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Size Variation ◽  
Genome Size Variation ◽  
Fusiform Rust

Genome size variation in Hordeum spontaneum populations

Genome ◽  
1999 ◽  
Vol 42 (6) ◽  
pp. 1094-1099 ◽  
Author(s):  
Timo Turpeinen ◽  
Johanna Kulmala ◽  
Eviatar Nevo
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Intraspecific Variation ◽  
Wild Barley ◽  
Size Variation ◽  
Hordeum Spontaneum ◽  
Genome Size Variation ◽  
Propidium Iodide Staining ◽  
Significant Difference ◽  
Positive Correlations

Populations of wild barley, Hordeum spontaneum (C. Koch), originating from 10 ecologically and geographically different sites in Israel, were assessed for genome size. Measurements were obtained by flow cytometry using propidium iodide staining. Genome sizes ranged from 9.35 to 9.81 pg. Variance analysis indicated a significant difference between populations. Genome sizes were positively correlated with mean January temperature. Our results corroborate previous findings of intraspecific variation in genome size from different plant species. The positive correlations between climate and genome size suggest that the latter is adaptive and determined by natural selection.Key words: Hordeum spontaneum, genome size, flow cytometry, intraspecific variation, natural selection.

Genome size variation in Begonia

Genome ◽  
2009 ◽  
Vol 52 (10) ◽  
pp. 829-838 ◽  
Author(s):  
Angelo Dewitte ◽  
Leen Leus ◽  
Tom Eeckhaut ◽  
Ives Vanstechelman ◽  
Johan Van Huylenbroeck ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Chromosome Number ◽  
Genome Size ◽  
Size Variation ◽  
South American ◽  
Chromosome Size ◽  
Genome Size Variation ◽  
Length Width ◽  
South American Origin ◽  
Secondary Constrictions

The genome sizes of a Begonia collection comprising 37 species and 23 hybrids of African, Asiatic, Middle American, and South American origin were screened using flow cytometry. Within the collection, 1C values varied between 0.23 and 1.46 pg DNA. Genome sizes were, in most cases, not positively correlated with chromosome number, but with pollen size. A 12-fold difference in mean chromosome size was found between the genotypes with the largest and smallest chromosomes. In general, chromosomes from South American genotypes were smaller than chromosomes of African, Asian, or Middle American genotypes, except for B. boliviensis and B. pearcei. Cytological chromosome studies in different genotypes showed variable chromosome numbers, length, width, and total chromosome volume, which confirmed the diversity in genome size. Large secondary constrictions were present in several investigated genotypes. These data show that chromosome number and structure exhibit a great deal of variation within the genus Begonia, and likely help to explain the large number of taxa found within the genus.

scholarly journals Intraspecific Genome Size Variation in Pumpkin (Cucurbita pepo subsp. pepo)

HortScience ◽  
2008 ◽  
Vol 43 (3) ◽  
pp. 949-951 ◽  
Author(s):  
A. Lane Rayburn ◽  
Mosbah M. Kushad ◽  
Wanisari Wannarat
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Cucurbita Pepo ◽  
Fruit Size ◽  
Size Variation ◽  
Fruit Morphology ◽  
Genome Size Variation ◽  
A Genome ◽  
Size Variability

Genome size has recently been reported to vary 16% in pumpkins (Cucurbita spp.). The majority of this variation can be attributed to genome size differences in pumpkins of various taxonomical classes. The purpose of this study was to determine if intraspecific genome size variability could be detected by flow cytometry in Cucurbita pepo subsp. pepo pumpkin cultivars with similar fruit morphology. The pie pumpkins group was chosen for this study because of their similar fruit size, shape, and color. Genome sizes ranged from 1.109 pg in Spooktacular to 1.064 pg in Small Sugar. Spooktacular had a genome size larger than Small Sugar in all three experiments. Therefore, intraspecific genome size variation does exist in C. pepo subsp. pepo among pumpkin cultivars of similar fruit morphology.

Survey of genome size in 28 hydrothermal vent species covering 10 families

Genome ◽  
2009 ◽  
Vol 52 (6) ◽  
pp. 524-536 ◽  
Author(s):  
Eric Bonnivard ◽  
Olivier Catrice ◽  
Juliette Ravaux ◽  
Spencer C. Brown ◽  
Dominique Higuet
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Hydrothermal Vent ◽  
Hydrothermal Vents ◽  
Size Variation ◽  
Genome Size Variation ◽  
Squat Lobster ◽  
Living Species ◽  
Ploidy Changes ◽  
Gains And Losses

Knowledge of genome size is a useful and necessary prerequisite for the development of many genomic resources. To better understand the origins and effects of DNA gains and losses among species, it is important to collect data from a broad taxonomic base, but also from particular ecosystems. Oceanic thermal vents are an interesting model to investigate genome size in very unstable environments. Here we provide data estimated by flow cytometry for 28 vent-living species among the most representative from different hydrothermal vents. We also report the genome size of closely related coastal decapods. Haploid C-values were compared with those previously reported for species from corresponding orders or infraorders. This is the first broad survey of 2C values in vent organisms. Contrary to expectations, it shows that certain hydrothermal vent species have particularly large genomes. The vent squat lobster Munidopsis recta has the largest genome yet reported for any anomuran: 2C = 31.1 pg = 30.4 × 109 bp. In several groups, such as Brachyura, Phyllodocida, and Veneroida, vent species have genomes that clearly rank at the high end of published values for each group. We also describe the highest DNA content yet recorded for the Brachyura (coastal crabs Xantho pilipes and Necora puber ). Finally, analysis of genome size variation across populations revealed unexpected intraspecific variation in the vent shrimp Mirocaris fortunata that could not be attributed simply to ploidy changes.

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