Cloning and sequencing of the 3-phosphoglycerate kinase (PGK) gene from Penicillium citrinum and its application to heterologous gene expression

1993 ◽  
Vol 23 (2) ◽  
pp. 134-140 ◽  
Author(s):  
Futoshi Nara ◽  
Ichiro Watanabe ◽  
Nobufusa Serizawa
Keyword(s):  
Gene Expression ◽  
Heterologous Gene Expression ◽  
Phosphoglycerate Kinase ◽  
Penicillium Citrinum ◽  
Heterologous Gene ◽  
Cloning And Sequencing

Cloning and characterization of two 3-phosphoglycerate kinase genes of Rhizopus niveus and heterologous gene expression using their promoters

1994 ◽  
Vol 25 (6) ◽  
pp. 524-530 ◽  
Author(s):  
Naoki Takaya ◽  
Koji Yanai ◽  
Hiroyuki Horiuchi ◽  
Akinori Ohta ◽  
Masamichi Takagi
Keyword(s):  
Gene Expression ◽  
Heterologous Gene Expression ◽  
Phosphoglycerate Kinase ◽  
Heterologous Gene ◽  
Rhizopus Niveus

scholarly journals Optimization of Heterologous Gene Expression for In Vitro Evolution

BioTechniques ◽  
2001 ◽  
Vol 30 (3) ◽  
pp. 474-476 ◽  
Author(s):  
Ichiro Matsumura ◽  
Mark J. Olsen ◽  
Andrew D. Ellington
Keyword(s):  
Gene Expression ◽  
Heterologous Gene Expression ◽  
Heterologous Gene ◽  
In Vitro Evolution

Production of foreign proteins in the yeast Pichia pastoris

1995 ◽  
Vol 73 (S1) ◽  
pp. 891-897 ◽  
Author(s):  
James M. Cregg ◽  
David R. Higgins
Keyword(s):  
Gene Expression ◽  
Pichia Pastoris ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Methylotrophic Yeast ◽  
Culture Broth ◽  
Heterologous Gene ◽  
Foreign Gene ◽  
Heterologous Proteins ◽  
Yeast Pichia Pastoris

The methanol-utilizing yeast Pichia pastoris has been developed as a host system for the production of heterologous proteins of commercial interest. An industrial yeast selected for efficient growth on methanol for biomass generation, P. pastoris is readily grown on defined medium in continuous culture at high volume and density. A unique feature of the expression system is the promoter employed to drive heterologous gene expression, which is derived from the methanol-regulated alcohol oxidase I gene (AOX1) of P. pastoris, one of the most efficient and tightly regulated promoters known. The strength of the AOX1 promoter results in high expression levels in strains harboring only a single integrated copy of a foreign-gene expression cassette. Levels may often be further enhanced through the integration of multiple cassette copies into the P. pastoris genome and strategies to construct and select multicopy cassette strains have been devised. The system is particularly attractive for the secretion of foreign-gene products. Because P. pastoris endogenous protein secretion levels are low, foreign secreted proteins often appear to be virtually the only proteins in the culture broth, a major advantage in processing and purification. Key words: heterologous gene expression, methylotrophic yeast, Pichia pastoris, secretion, glycosylation.

Can heterologous gene expression shed (a torch) light on protein function?

2004 ◽  
Vol 22 (11) ◽  
pp. 557-559 ◽  
Author(s):  
Pascal Dubessay ◽  
Michel Pagès ◽  
Frédéric Delbac ◽  
Patrick Bastien ◽  
Christian Vivares ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Function ◽  
Heterologous Gene Expression ◽  
Heterologous Gene

scholarly journals An overview on current molecular tools for heterologous gene expression in Trichoderma

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Irene Tomico-Cuenca ◽  
Robert L. Mach ◽  
Astrid R. Mach-Aigner ◽  
Christian Derntl
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Protein Secretion ◽  
Heterologous Gene Expression ◽  
Heterologous Gene ◽  
Molecular Tools ◽  
Expression Systems ◽  
Industrial Enzymes ◽  
Nutrient Sources ◽  
Transformation Methods

AbstractFungi of the genus Trichoderma are routinely used as biocontrol agents and for the production of industrial enzymes. Trichoderma spp. are interesting hosts for heterologous gene expression because their saprotrophic and mycoparasitic lifestyles enable them to thrive on a large number of nutrient sources and some members of this genus are generally recognized as safe (GRAS status). In this review, we summarize and discuss several aspects involved in heterologous gene expression in Trichoderma, including transformation methods, genome editing strategies, native and synthetic expression systems and implications of protein secretion. This review focuses on the industrial workhorse Trichoderma reesei because this fungus is the best-studied member of this genus for protein expression and secretion. However, the discussed strategies and tools can be expected to be transferable to other Trichoderma species.

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