Cloning and characterization of two 3-phosphoglycerate kinase genes of Rhizopus niveus and heterologous gene expression using their promoters

1994 ◽  
Vol 25 (6) ◽  
pp. 524-530 ◽  
Author(s):  
Naoki Takaya ◽  
Koji Yanai ◽  
Hiroyuki Horiuchi ◽  
Akinori Ohta ◽  
Masamichi Takagi
Keyword(s):  
Gene Expression ◽  
Heterologous Gene Expression ◽  
Phosphoglycerate Kinase ◽  
Heterologous Gene ◽  
Rhizopus Niveus

scholarly journals Characterization of the Human Artemis Promoter by Heterologous Gene Expression In Vitro and In Vivo

2011 ◽  
Vol 30 (10) ◽  
pp. 751-761 ◽  
Author(s):  
Megan M. Multhaup ◽  
Sweta Gurram ◽  
Kelly M. Podetz-Pedersen ◽  
Andrea D. Karlen ◽  
Debra L. Swanson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heterologous Gene Expression ◽  
Heterologous Gene

Heterologous gene expression and characterization of two serine hydroxymethyltransferases from Thermoplasma acidophilum

Extremophiles ◽  
2021 ◽  
Author(s):  
Ilma Fauziah Ma’ruf ◽  
Yuka Sasaki ◽  
Anastasia Kerbs ◽  
Jochen Nießer ◽  
Yu Sato ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heterologous Gene Expression ◽  
Heterologous Gene ◽  
Thermoplasma Acidophilum

Characterization of chromosomal integration sites for heterologous gene expression inSaccharomyces cerevisiae

Yeast ◽  
2009 ◽  
Vol 26 (10) ◽  
pp. 545-551 ◽  
Author(s):  
Dongmei Bai Flagfeldt ◽  
Verena Siewers ◽  
Le Huang ◽  
Jens Nielsen
Keyword(s):  
Gene Expression ◽  
Heterologous Gene Expression ◽  
Heterologous Gene ◽  
Chromosomal Integration ◽  
Integration Sites

scholarly journals Among Multiple Phosphomannomutase Gene Orthologues, Only One Gene Encodes a Protein with Phosphoglucomutase and Phosphomannomutase Activities in Thermococcus kodakaraensis

2004 ◽  
Vol 186 (18) ◽  
pp. 6070-6076 ◽  
Author(s):  
Naeem Rashid ◽  
Tamotsu Kanai ◽  
Haruyuki Atomi ◽  
Tadayuki Imanaka
Keyword(s):  
Gene Expression ◽  
Recombinant Protein ◽  
Heterologous Gene Expression ◽  
Heterologous Gene ◽  
Expression And Purification ◽  
Purification And Characterization ◽  
Orthologous Genes ◽  
Hyperthermophilic Archaeon ◽  
In Cells

ABSTRACT Four orthologous genes (TK1108, TK1404, TK1777, and TK2185) that can be annotated as phosphomannomutase (PMM) genes (COG1109) have been identified in the genome of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. We previously found that TK1777 actually encodes a phosphopentomutase. In order to determine which of the remaining three orthologues encodes a phosphoglucomutase (PGM), we examined the PGM activity in T. kodakaraensis cells and identified the gene responsible for this activity. Heterologous gene expression and purification and characterization of the recombinant protein indicated that TK1108 encoded a protein with high levels of PGM activity (690 U mg−1), along with high levels of PMM activity (401 U mg−1). Similar analyses of the remaining two orthologues revealed that their protein products exhibited neither PGM nor PMM activity. PGM activity and transcription of TK1108 in T. kodakaraensis were found to be higher in cells grown on starch than in cells grown on pyruvate. Our results clearly indicate that, among the four PMM gene orthologues in T. kodakaraensis, only one gene, TK1108, actually encodes a protein with PGM and PMM activities.

scholarly journals Optimization of Heterologous Gene Expression for In Vitro Evolution

BioTechniques ◽  
2001 ◽  
Vol 30 (3) ◽  
pp. 474-476 ◽  
Author(s):  
Ichiro Matsumura ◽  
Mark J. Olsen ◽  
Andrew D. Ellington
Keyword(s):  
Gene Expression ◽  
Heterologous Gene Expression ◽  
Heterologous Gene ◽  
In Vitro Evolution
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