Constitutive homologous recombination between mitochondrial DNA and a linear mitochondrial plasmid in Physarum polycephalum

1992 ◽  
Vol 22 (3) ◽  
pp. 221-227 ◽  
Author(s):  
Hiroyoshi Takano ◽  
Shigeyuki Kawano ◽  
Tsyneyoshi Kuroiwa
Keyword(s):  
Mitochondrial Dna ◽  
Homologous Recombination ◽  
Physarum Polycephalum ◽  
Mitochondrial Plasmid ◽  
Linear Mitochondrial Plasmid

Telomeric structures in a linear mitochondrial plasmid from Physarum polycephalum

1991 ◽  
Vol 20 (4) ◽  
pp. 315-317 ◽  
Author(s):  
Hiroyoshi Takano ◽  
Shigeyuki Kawano ◽  
Tsuneyoshi Kuroiwa
Keyword(s):  
Physarum Polycephalum ◽  
Mitochondrial Plasmid ◽  
Linear Mitochondrial Plasmid

A mitochondrial retroplasmid integrates into mitochondrial DNA by a novel mechanism involving the synthesis of a hybrid cDNA and homologous recombination

1994 ◽  
Vol 14 (10) ◽  
pp. 6419-6432
Author(s):  
C C Chiang ◽  
J C Kennell ◽  
L A Wanner ◽  
A M Lambowitz
Keyword(s):  
Mitochondrial Dna ◽  
Homologous Recombination ◽  
Rrna Gene ◽  
Template Switching ◽  
Transcript Analysis ◽  
Wild Type ◽  
Mitochondrial Trna ◽  
Integration Mechanism ◽  
Circular Dnas ◽  
Simple Integration

The Mauriceville and Varkud mitochondrial plasmids of Neurospora spp. are closely related, small circular DNAs that propagate via an RNA intermediate and reverse transcription. Although the plasmids ordinarily replicate autonomously, they can also integrate into mitochondrial DNA (mtDNA), yielding defective mtDNAs that in some cases cause senescence. To investigate the integration mechanism, we analyzed four cases in which the Varkud plasmid integrated into the mitochondrial small rRNA gene, three in wild-type subcultures and one in a senescent mutant. Our analysis suggests that the integrations occurred by the plasmid reverse transcriptase template switching between the plasmid transcript and internal sequences in the mitochondrial small rRNA to yield hybrid cDNAs that circularized and recombined homologously with the mtDNA. The integrated plasmid sequences are transcribed, presumably from the mitochondrial small rRNA promoters, resulting in hybrid RNAs containing the 5' segment of the mitochondrial small rRNA linked head-to-tail to the full-length plasmid transcript. Analysis of additional senescent mutants revealed three cases in which the plasmid used the same mechanism to integrate at other locations in the mtDNA. In these cases, circular variant plasmids that had incorporated a mitochondrial tRNA or tRNA-like sequence by template switching integrated by homologous recombination at the site of the corresponding tRNA or tRNA-like sequence in mtDNA. This simple integration mechanism involving template switching to generate a hybrid cDNA that integrates homologously could have been used by primitive retroelements prior to the acquisition of a specialized integration machinery.

scholarly journals The Mitochondrial DNA (mtDNA)-Associated Protein SWIB5 Influences mtDNA Architecture and Homologous Recombination

2017 ◽  
pp. tpc.00899.2016 ◽  
Author(s):  
Jonas Blomme ◽  
Olivier Van Aken ◽  
Jelle Van Leene ◽  
Teddy Jégu ◽  
Riet Maria De Rycke ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Homologous Recombination
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