Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum (Gibberella pulicaris)

1992 ◽  
Vol 22 (3) ◽  
pp. 213-220 ◽  
Author(s):  
Kerry O'Donnell
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacers ◽  
Fusarium Sambucinum ◽  
Gibberella Pulicaris

scholarly journals Potentially dangerous fusarioid microorganisms associated with rot of hops (Humulus lupulus L.) plants in field culture

2008 ◽  
Vol 54 (No. 4) ◽  
pp. 149-154 ◽  
Author(s):  
M. Gryndler ◽  
K. Krofta ◽  
H. Gryndlerová ◽  
L. Soukupová ◽  
H. Hršelová ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Humulus Lupulus ◽  
Plant Tissues ◽  
Fusarium Sambucinum ◽  
Specific Primer ◽  
Gibberella Pulicaris ◽  
Field Culture ◽  
Restriction Fragment Length

Several fusarioid microorganisms were isolated as potential pathogens of hop (<i>Humulus lupulus</I> L.) but their virulence was not proved in inoculation trials in field conditions. Molecular search for other possible pathogens was then performed. Using terminal restriction fragment length polymorphism (TRFLP), <i>Gibberella pulicaris</I> (anamorph: <i>Fusarium sambucinum</I>) was identified as a probable cause of the hop wilting. The primary cause of the disease is wounding of hop crowns by feeding of rosy rustic moth (<i>Hydraecia micacea</I>) caterpillars or by defect pruning and other unfavourable circumstances. The specific primer HLf1 was designed that can be used to detect the pathogen in soil and in damaged plant tissues.

Characterization of Trichuris trichiura from humans and T. suis from pigs in China using internal transcribed spacers of nuclear ribosomal DNA

2012 ◽  
Vol 88 (1) ◽  
pp. 64-68 ◽  
Author(s):  
G.H. Liu ◽  
W. Zhou ◽  
A.J. Nisbet ◽  
M.J. Xu ◽  
D.H. Zhou ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Sequence Variation ◽  
Animal Health ◽  
Trichuris Trichiura ◽  
Its Rdna ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Economic Losses ◽  
Molecular Characteristics ◽  
Rdna Sequences

AbstractTrichuris trichiura and Trichuris suis parasitize (at the adult stage) the caeca of humans and pigs, respectively, causing trichuriasis. Despite these parasites being of human and animal health significance, causing considerable socio-economic losses globally, little is known of the molecular characteristics of T. trichiura and T. suis from China. In the present study, the entire first and second internal transcribed spacer (ITS-1 and ITS-2) regions of nuclear ribosomal DNA (rDNA) of T. trichiura and T. suis from China were amplified by polymerase chain reaction (PCR), the representative amplicons were cloned and sequenced, and sequence variation in the ITS rDNA was examined. The ITS rDNA sequences for the T. trichiura and T. suis samples were 1222–1267 bp and 1339–1353 bp in length, respectively. Sequence analysis revealed that the ITS-1, 5.8S and ITS-2 rDNAs of both whipworms were 600–627 bp and 655–661 bp, 154 bp, and 468–486 bp and 530–538 bp in size, respectively. Sequence variation in ITS rDNA within and among T. trichiura and T. suis was examined. Excluding nucleotide variations in the simple sequence repeats, the intra-species sequence variation in the ITS-1 was 0.2–1.7% within T. trichiura, and 0–1.5% within T. suis. For ITS-2 rDNA, the intra-species sequence variation was 0–1.3% within T. trichiura and 0.2–1.7% within T. suis. The inter-species sequence differences between the two whipworms were 60.7–65.3% for ITS-1 and 59.3–61.5% for ITS-2. These results demonstrated that the ITS rDNA sequences provide additional genetic markers for the characterization and differentiation of the two whipworms. These data should be useful for studying the epidemiology and population genetics of T. trichiura and T. suis, as well as for the diagnosis of trichuriasis in humans and pigs.

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