scholarly journals Origin and evolution of the endemic genera of Gonosperminae (Asteraceae: Anthemideae) from the Canary Islands: evidence from nucleotide sequences of the internal transcribed spacers of the nuclear ribosomal DNA

2001 ◽  
Vol 88 (1) ◽  
pp. 161-169 ◽  
Author(s):  
Javier Francisco-Ortega ◽  
Janet C. Barber ◽  
Arnoldo Santos-Guerra ◽  
Rosa Febles-Hernández ◽  
Robert K. Jansen
Keyword(s):  
Canary Islands ◽  
Ribosomal Dna ◽  
Nucleotide Sequences ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Origin And Evolution ◽  
Endemic Genera

scholarly journals Origin and evolution of the endemic Macaronesian Inuleae (Asteraceae): evidence from the internal transcribed spacers of nuclear ribosomal DNA

2001 ◽  
Vol 72 (1) ◽  
pp. 77-97 ◽  
Author(s):  
JAVIER FRANCISCO-ORTEGA ◽  
SEON-JOO PARK ◽  
ARNOLDO SANTOS-GUERRA ◽  
ABDELMALEK BENABID ◽  
ROBERT K. JANSEN
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Origin And Evolution

Characterization of Trichuris trichiura from humans and T. suis from pigs in China using internal transcribed spacers of nuclear ribosomal DNA

2012 ◽  
Vol 88 (1) ◽  
pp. 64-68 ◽  
Author(s):  
G.H. Liu ◽  
W. Zhou ◽  
A.J. Nisbet ◽  
M.J. Xu ◽  
D.H. Zhou ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Sequence Variation ◽  
Animal Health ◽  
Trichuris Trichiura ◽  
Its Rdna ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Economic Losses ◽  
Molecular Characteristics ◽  
Rdna Sequences

AbstractTrichuris trichiura and Trichuris suis parasitize (at the adult stage) the caeca of humans and pigs, respectively, causing trichuriasis. Despite these parasites being of human and animal health significance, causing considerable socio-economic losses globally, little is known of the molecular characteristics of T. trichiura and T. suis from China. In the present study, the entire first and second internal transcribed spacer (ITS-1 and ITS-2) regions of nuclear ribosomal DNA (rDNA) of T. trichiura and T. suis from China were amplified by polymerase chain reaction (PCR), the representative amplicons were cloned and sequenced, and sequence variation in the ITS rDNA was examined. The ITS rDNA sequences for the T. trichiura and T. suis samples were 1222–1267 bp and 1339–1353 bp in length, respectively. Sequence analysis revealed that the ITS-1, 5.8S and ITS-2 rDNAs of both whipworms were 600–627 bp and 655–661 bp, 154 bp, and 468–486 bp and 530–538 bp in size, respectively. Sequence variation in ITS rDNA within and among T. trichiura and T. suis was examined. Excluding nucleotide variations in the simple sequence repeats, the intra-species sequence variation in the ITS-1 was 0.2–1.7% within T. trichiura, and 0–1.5% within T. suis. For ITS-2 rDNA, the intra-species sequence variation was 0–1.3% within T. trichiura and 0.2–1.7% within T. suis. The inter-species sequence differences between the two whipworms were 60.7–65.3% for ITS-1 and 59.3–61.5% for ITS-2. These results demonstrated that the ITS rDNA sequences provide additional genetic markers for the characterization and differentiation of the two whipworms. These data should be useful for studying the epidemiology and population genetics of T. trichiura and T. suis, as well as for the diagnosis of trichuriasis in humans and pigs.

Molecular Systematics of the Asteriscus Alliance (Asteraceae: Inuleae) I: Evidence from the Internal Transcribed Spacers of Nuclear Ribosomal DNA

1999 ◽  
Vol 24 (2) ◽  
pp. 249 ◽  
Author(s):  
Javier Francisco-Ortega ◽  
Leslie R. Goertzen ◽  
Arnoldo Santos-Guerra ◽  
Abdelmalek Benabid ◽  
Robert K. Jansen
Keyword(s):  
Molecular Systematics ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna
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