Separation of chromosomal DNA molecules from Phoma tracheiphila by orthogonal-field-alternation gel electrophoresis

1989 ◽  
Vol 16 (5-6) ◽  
pp. 477-479 ◽  
Author(s):  
Franco Rollo ◽  
Tina Ferracuti ◽  
Aurelio Pacilli
Keyword(s):  
Gel Electrophoresis ◽  
Dna Molecules ◽  
Chromosomal Dna ◽  
Phoma Tracheiphila ◽  
Orthogonal Field

scholarly journals An electrophoretic karyotype of Neurospora crassa.

1988 ◽  
Vol 8 (4) ◽  
pp. 1469-1473 ◽  
Author(s):  
M J Orbach ◽  
D Vollrath ◽  
R W Davis ◽  
C Yanofsky
Keyword(s):  
Neurospora Crassa ◽  
Electric Fields ◽  
Gel Electrophoresis ◽  
Electrophoretic Karyotype ◽  
Dna Molecules ◽  
Linkage Groups ◽  
Homogeneous Electric Field ◽  
Chromosomal Dna ◽  
Molecular Karyotype ◽  
Electrophoresis System

A molecular karyotype of Neurospora crassa was obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogeneous electric fields. The migration of all seven N. crassa chromosomal DNAs was defined, and five of the seven molecules were separated from one another. The estimated sizes of these molecules, based on their migration relative to Schizosaccharomyces pombe chromosomal DNA molecules, are 4 to 12.6 megabases. The seven linkage groups were correlated with specific chromosomal DNA bands by hybridizing transfers of contour-clamped homogeneous electric field gels with radioactive probes specific to each linkage group. The mobilities of minichromosomal DNAs generated from translocation strains were also examined. The methods used for preparation of chromosomal DNA molecules and the conditions for their separation should be applicable to other filamentous fungi.

scholarly journals Electrophoretic analysis of Histoplasma capsulatum chromosomal DNA.

1989 ◽  
Vol 9 (3) ◽  
pp. 983-987 ◽  
Author(s):  
P E Steele ◽  
G F Carle ◽  
G S Kobayashi ◽  
G Medoff
Keyword(s):  
Gel Electrophoresis ◽  
Histoplasma Capsulatum ◽  
Electrophoretic Analysis ◽  
Considerable Variability ◽  
Dna Molecules ◽  
Homogeneous Electric Field ◽  
Chromosomal Dna ◽  
Experimental Conditions ◽  
Agarose Gels ◽  
Minimum Estimate

Seven chromosome-sized DNA molecules in the Downs strain of Histoplasma capsulatum were resolved by using chromosome-specific DNA probes in blot hybridizations of contour-clamped homogeneous electric field (CHEF) and field-inversion gel electrophoresis (FIGE) agarose gels. The sizes of the chromosomal DNA bands extended from that of the largest Saccharomyces cerevisiae chromosome to beyond that of the Schizosaccharomyces pombe chromosomes. Under our experimental conditions, the order of the five largest DNA bands was inverted in the FIGE gel relative to the CHEF gel, demonstrating a characteristic of FIGE whereby large DNA molecules may have greater rather than lesser mobility with increasing size. Comparison of the Downs strain with other H. capsulatum strains by CHEF and FIGE analysis revealed considerable variability in band mobility. The resolution of seven chromosome-sized DNA molecules in the Downs strain provides a minimum estimate of the chromosome number.

scholarly journals Macronuclear DNA molecules of Tetrahymena thermophila.

1986 ◽  
Vol 6 (3) ◽  
pp. 900-905 ◽  
Author(s):  
R K Conover ◽  
C F Brunk
Keyword(s):  
Gel Electrophoresis ◽  
Molecular Mechanisms ◽  
Tetrahymena Thermophila ◽  
Fragmentation Process ◽  
Dna Molecules ◽  
Physical Organization ◽  
Different Strains ◽  
Average Size ◽  
Orthogonal Field

The physical organization of the DNA in the macronuclei of Tetrahymena thermophila was investigated by using alternating-orthogonal-field gel electrophoresis. The genome consisted of a spectrum of molecules with lengths ranging from less than 100 to in excess of 1,500 kilobase pairs. There were about 270 different macronuclear DNA molecules, with an average size of about 800 kilobase pairs. Specific genes were mapped and were generally found on macronuclear DNA molecules of the same size in different strains of T. thermophila. This indicates that the molecular mechanisms giving rise to the macronuclear DNA molecules were precise. The fragmentation process that gave rise to macronuclear DNA molecules occurred between 11 and 19 h after the initiation of conjugation.

Electrophoretic analysis of Histoplasma capsulatum chromosomal DNA

1989 ◽  
Vol 9 (3) ◽  
pp. 983-987
Author(s):  
P E Steele ◽  
G F Carle ◽  
G S Kobayashi ◽  
G Medoff
Keyword(s):  
Gel Electrophoresis ◽  
Histoplasma Capsulatum ◽  
Electrophoretic Analysis ◽  
Considerable Variability ◽  
Dna Molecules ◽  
Homogeneous Electric Field ◽  
Chromosomal Dna ◽  
Experimental Conditions ◽  
Agarose Gels ◽  
Minimum Estimate

Seven chromosome-sized DNA molecules in the Downs strain of Histoplasma capsulatum were resolved by using chromosome-specific DNA probes in blot hybridizations of contour-clamped homogeneous electric field (CHEF) and field-inversion gel electrophoresis (FIGE) agarose gels. The sizes of the chromosomal DNA bands extended from that of the largest Saccharomyces cerevisiae chromosome to beyond that of the Schizosaccharomyces pombe chromosomes. Under our experimental conditions, the order of the five largest DNA bands was inverted in the FIGE gel relative to the CHEF gel, demonstrating a characteristic of FIGE whereby large DNA molecules may have greater rather than lesser mobility with increasing size. Comparison of the Downs strain with other H. capsulatum strains by CHEF and FIGE analysis revealed considerable variability in band mobility. The resolution of seven chromosome-sized DNA molecules in the Downs strain provides a minimum estimate of the chromosome number.

scholarly journals Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae

1998 ◽  
Vol 21 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Maria Cléria Valadares-Inglis ◽  
John F. Peberdy
Keyword(s):  
Genome Size ◽  
Metarhizium Anisopliae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
The Other ◽  
Electrophoretic Karyotype ◽  
Dna Molecules ◽  
Chromosomal Dna ◽  
Pulsed Field ◽  
Densitometric Analysis

Pulsed-field gel electrophoresis (PFGE) was used to separate chromosome-sized DNA molecules of four strains of Metarhizium anisopliae from Brazil. Metarhizium anisopliae isolates from Japan have been reported as possessing seven chromosomes. Variation was observed among the Brazilian strains and the chromosomal DNA was resolved into eight bands for strain CG46. Densitometric analysis of PFGE gels suggested that the other three Brazilian strains also possess eight chromosomes, with two chromosomes migrating as doublets under the electrophoretic conditions used. The genome size was estimated as varying between 23.39 to 31.88 Mb, not including possible doublet chromosomes.

Imaging of kinked configurations of DNA molecules undergoing orthogonal field alternating gel electrophoresis by fluorescence microscopy

Biochemistry ◽  
1990 ◽  
Vol 29 (13) ◽  
pp. 3396-3401 ◽  
Author(s):  
Sergio Gurrieri ◽  
Enrico Rizzarelli ◽  
David Beach ◽  
Carlos Bustamante
Keyword(s):  
Fluorescence Microscopy ◽  
Gel Electrophoresis ◽  
Dna Molecules ◽  
Orthogonal Field

Macronuclear DNA molecules of Tetrahymena thermophila

1986 ◽  
Vol 6 (3) ◽  
pp. 900-905
Author(s):  
R K Conover ◽  
C F Brunk
Keyword(s):  
Gel Electrophoresis ◽  
Molecular Mechanisms ◽  
Tetrahymena Thermophila ◽  
Fragmentation Process ◽  
Dna Molecules ◽  
Physical Organization ◽  
Different Strains ◽  
Average Size ◽  
Orthogonal Field

The physical organization of the DNA in the macronuclei of Tetrahymena thermophila was investigated by using alternating-orthogonal-field gel electrophoresis. The genome consisted of a spectrum of molecules with lengths ranging from less than 100 to in excess of 1,500 kilobase pairs. There were about 270 different macronuclear DNA molecules, with an average size of about 800 kilobase pairs. Specific genes were mapped and were generally found on macronuclear DNA molecules of the same size in different strains of T. thermophila. This indicates that the molecular mechanisms giving rise to the macronuclear DNA molecules were precise. The fragmentation process that gave rise to macronuclear DNA molecules occurred between 11 and 19 h after the initiation of conjugation.

An electrophoretic karyotype of Neurospora crassa

1988 ◽  
Vol 8 (4) ◽  
pp. 1469-1473 ◽  
Author(s):  
M J Orbach ◽  
D Vollrath ◽  
R W Davis ◽  
C Yanofsky
Keyword(s):  
Neurospora Crassa ◽  
Electric Fields ◽  
Gel Electrophoresis ◽  
Electrophoretic Karyotype ◽  
Dna Molecules ◽  
Linkage Groups ◽  
Homogeneous Electric Field ◽  
Chromosomal Dna ◽  
Molecular Karyotype ◽  
Electrophoresis System

A molecular karyotype of Neurospora crassa was obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogeneous electric fields. The migration of all seven N. crassa chromosomal DNAs was defined, and five of the seven molecules were separated from one another. The estimated sizes of these molecules, based on their migration relative to Schizosaccharomyces pombe chromosomal DNA molecules, are 4 to 12.6 megabases. The seven linkage groups were correlated with specific chromosomal DNA bands by hybridizing transfers of contour-clamped homogeneous electric field gels with radioactive probes specific to each linkage group. The mobilities of minichromosomal DNAs generated from translocation strains were also examined. The methods used for preparation of chromosomal DNA molecules and the conditions for their separation should be applicable to other filamentous fungi.

scholarly journals Capture and elongation of single chromosomal DNA molecules using optically driven microchopsticks

2020 ◽  
Vol 14 (4) ◽  
pp. 044114
Author(s):  
Ryo Inukai ◽  
Hidekuni Takao ◽  
Fusao Shimokawa ◽  
Kyohei Terao
Keyword(s):  
Dna Molecules ◽  
Chromosomal Dna
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