scholarly journals Capture and elongation of single chromosomal DNA molecules using optically driven microchopsticks

2020 ◽  
Vol 14 (4) ◽  
pp. 044114
Author(s):  
Ryo Inukai ◽  
Hidekuni Takao ◽  
Fusao Shimokawa ◽  
Kyohei Terao
Keyword(s):  
Dna Molecules ◽  
Chromosomal Dna

scholarly journals An electrophoretic karyotype of Neurospora crassa.

1988 ◽  
Vol 8 (4) ◽  
pp. 1469-1473 ◽  
Author(s):  
M J Orbach ◽  
D Vollrath ◽  
R W Davis ◽  
C Yanofsky
Keyword(s):  
Neurospora Crassa ◽  
Electric Fields ◽  
Gel Electrophoresis ◽  
Electrophoretic Karyotype ◽  
Dna Molecules ◽  
Linkage Groups ◽  
Homogeneous Electric Field ◽  
Chromosomal Dna ◽  
Molecular Karyotype ◽  
Electrophoresis System

A molecular karyotype of Neurospora crassa was obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogeneous electric fields. The migration of all seven N. crassa chromosomal DNAs was defined, and five of the seven molecules were separated from one another. The estimated sizes of these molecules, based on their migration relative to Schizosaccharomyces pombe chromosomal DNA molecules, are 4 to 12.6 megabases. The seven linkage groups were correlated with specific chromosomal DNA bands by hybridizing transfers of contour-clamped homogeneous electric field gels with radioactive probes specific to each linkage group. The mobilities of minichromosomal DNAs generated from translocation strains were also examined. The methods used for preparation of chromosomal DNA molecules and the conditions for their separation should be applicable to other filamentous fungi.

scholarly journals Electrophoretic analysis of Histoplasma capsulatum chromosomal DNA.

1989 ◽  
Vol 9 (3) ◽  
pp. 983-987 ◽  
Author(s):  
P E Steele ◽  
G F Carle ◽  
G S Kobayashi ◽  
G Medoff
Keyword(s):  
Gel Electrophoresis ◽  
Histoplasma Capsulatum ◽  
Electrophoretic Analysis ◽  
Considerable Variability ◽  
Dna Molecules ◽  
Homogeneous Electric Field ◽  
Chromosomal Dna ◽  
Experimental Conditions ◽  
Agarose Gels ◽  
Minimum Estimate

Seven chromosome-sized DNA molecules in the Downs strain of Histoplasma capsulatum were resolved by using chromosome-specific DNA probes in blot hybridizations of contour-clamped homogeneous electric field (CHEF) and field-inversion gel electrophoresis (FIGE) agarose gels. The sizes of the chromosomal DNA bands extended from that of the largest Saccharomyces cerevisiae chromosome to beyond that of the Schizosaccharomyces pombe chromosomes. Under our experimental conditions, the order of the five largest DNA bands was inverted in the FIGE gel relative to the CHEF gel, demonstrating a characteristic of FIGE whereby large DNA molecules may have greater rather than lesser mobility with increasing size. Comparison of the Downs strain with other H. capsulatum strains by CHEF and FIGE analysis revealed considerable variability in band mobility. The resolution of seven chromosome-sized DNA molecules in the Downs strain provides a minimum estimate of the chromosome number.

The form of chromosomal DNA molecules in bacterial cells

Biochimie ◽  
2001 ◽  
Vol 83 (2) ◽  
pp. 177-186 ◽  
Author(s):  
Arnold J Bendich
Keyword(s):  
Bacterial Cells ◽  
Dna Molecules ◽  
Chromosomal Dna

Electrophoretic analysis of Histoplasma capsulatum chromosomal DNA

1989 ◽  
Vol 9 (3) ◽  
pp. 983-987
Author(s):  
P E Steele ◽  
G F Carle ◽  
G S Kobayashi ◽  
G Medoff
Keyword(s):  
Gel Electrophoresis ◽  
Histoplasma Capsulatum ◽  
Electrophoretic Analysis ◽  
Considerable Variability ◽  
Dna Molecules ◽  
Homogeneous Electric Field ◽  
Chromosomal Dna ◽  
Experimental Conditions ◽  
Agarose Gels ◽  
Minimum Estimate

Seven chromosome-sized DNA molecules in the Downs strain of Histoplasma capsulatum were resolved by using chromosome-specific DNA probes in blot hybridizations of contour-clamped homogeneous electric field (CHEF) and field-inversion gel electrophoresis (FIGE) agarose gels. The sizes of the chromosomal DNA bands extended from that of the largest Saccharomyces cerevisiae chromosome to beyond that of the Schizosaccharomyces pombe chromosomes. Under our experimental conditions, the order of the five largest DNA bands was inverted in the FIGE gel relative to the CHEF gel, demonstrating a characteristic of FIGE whereby large DNA molecules may have greater rather than lesser mobility with increasing size. Comparison of the Downs strain with other H. capsulatum strains by CHEF and FIGE analysis revealed considerable variability in band mobility. The resolution of seven chromosome-sized DNA molecules in the Downs strain provides a minimum estimate of the chromosome number.

scholarly journals Variation in the electrophoretic karyotype of Brazilian strains of Metarhizium anisopliae

1998 ◽  
Vol 21 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Maria Cléria Valadares-Inglis ◽  
John F. Peberdy
Keyword(s):  
Genome Size ◽  
Metarhizium Anisopliae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
The Other ◽  
Electrophoretic Karyotype ◽  
Dna Molecules ◽  
Chromosomal Dna ◽  
Pulsed Field ◽  
Densitometric Analysis

Pulsed-field gel electrophoresis (PFGE) was used to separate chromosome-sized DNA molecules of four strains of Metarhizium anisopliae from Brazil. Metarhizium anisopliae isolates from Japan have been reported as possessing seven chromosomes. Variation was observed among the Brazilian strains and the chromosomal DNA was resolved into eight bands for strain CG46. Densitometric analysis of PFGE gels suggested that the other three Brazilian strains also possess eight chromosomes, with two chromosomes migrating as doublets under the electrophoretic conditions used. The genome size was estimated as varying between 23.39 to 31.88 Mb, not including possible doublet chromosomes.

scholarly journals Development of gene mapping for single chromosomal DNA molecules

1999 ◽  
Vol 119 (12) ◽  
pp. 620-625 ◽  
Author(s):  
Yasunori Matsui ◽  
Kazunori Takashima ◽  
Shinji Katsura ◽  
Akira Mizuno
Keyword(s):  
Gene Mapping ◽  
Dna Molecules ◽  
Chromosomal Dna

scholarly journals Packaging specific segments of the Salmonella chromosome with locked-in Mud-P22 prophages.

Genetics ◽  
1988 ◽  
Vol 118 (4) ◽  
pp. 581-592
Author(s):  
P Youderian ◽  
P Sugiono ◽  
K L Brewer ◽  
N P Higgins ◽  
T Elliott
Keyword(s):  
Homologous Recombination ◽  
Genetic Markers ◽  
Dna Molecules ◽  
Chromosomal Dna ◽  
Genetic Elements ◽  
Phage P22 ◽  
Salmonella Phage

Abstract Hybrid genetic elements, Mud-P and Mud-Q (collectively, Mud-P22s), have been constructed that carry two-thirds of the temperate Salmonella phage P22 genome sandwiched between the ends of transposon Mu. Insertions of these elements in the Salmonella chromosome generate locked-in P22 prophages that cannot excise. Upon induction (as a consequence of the inactivation of P22 c2 repressor), a locked-in prophage replicates its DNA in situ, resulting in the amplification of neighboring regions of the chromosome and the processive packaging of three contiguous headsful of adjacent DNA in one direction from the P22 packaging site, pac. Phage particles in an induced lysate of a Mud-P22 lysogen contain DNA molecules corresponding to several minutes of chromosomal DNA adjacent to the site of prophage insertion and transduce nearby genetic markers with high efficiencies. Mud-P22 prophages have been introduced into an F' episome by transposition; resident Mud insertions on the Salmonella chromosome may be converted to Mud-P22 insertions by homologous recombination in P22-mediated transductional crosses.

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