Detection of a single-copy gene on plant chromosomes by in situ hybridization

1988 ◽  
Vol 211 (1) ◽  
pp. 143-147 ◽  
Author(s):  
Pung-Ling Huang ◽  
Klaus Hahlbrock ◽  
Imre E. Somssich
Keyword(s):  
In Situ Hybridization ◽  
Single Copy ◽  
Single Copy Gene ◽  
Plant Chromosomes ◽  
Copy Gene

scholarly journals Single-copy Gene Detection Using Branched DNA (bDNA) In Situ Hybridization

2001 ◽  
Vol 49 (5) ◽  
pp. 603-611 ◽  
Author(s):  
Audrey N. Player ◽  
Lu-Ping Shen ◽  
Daryn Kenny ◽  
Vincent P. Antao ◽  
Janice A. Kolberg
Keyword(s):  
In Situ Hybridization ◽  
Single Copy ◽  
Single Copy Gene ◽  
Gene Detection ◽  
Branched Dna ◽  
Copy Gene

In situ amplification of single copy gene segments in individual cells by the polymerase chain reaction

Infection ◽  
1991 ◽  
Vol 19 (4) ◽  
pp. 242-244 ◽  
Author(s):  
W. Spann ◽  
Katharina Pachmann ◽  
Halina Zabnienska ◽  
Andrea Pielmeier ◽  
B. Emmerich
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Copy ◽  
Single Copy Gene ◽  
Chain Reaction ◽  
Copy Gene ◽  
Polymerase Chain

Identification of a single-copy gene encoding a Type I chlorophyll a/b-binding polypeptide of photosystem I in Arabidopsis thaliana

1992 ◽  
Vol 84 (4) ◽  
pp. 561-567 ◽  
Author(s):  
Poul E. Jensen ◽  
Michael Kristensen ◽  
Tine Hoff ◽  
Jan Lehmbeck ◽  
Bjarne M. Stummann ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Chlorophyll A ◽  
Photosystem I ◽  
Single Copy ◽  
Type I ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene

A Mouse Single-Copy Gene,Gtf2i,the Homolog of HumanGTF2I,That Is Duplicated in the Williams–Beuren Syndrome Deletion Region

Genomics ◽  
1998 ◽  
Vol 48 (2) ◽  
pp. 163-170 ◽  
Author(s):  
Yu-Ker Wang ◽  
Luis A. Pérez-Jurado ◽  
Uta Francke
Keyword(s):  
Single Copy ◽  
Single Copy Gene ◽  
Deletion Region ◽  
Copy Gene

scholarly journals Real-Time PCR for Molecular Detection of Zoonotic and Non-Zoonotic Giardia spp. in Wild Rodents

2021 ◽  
Vol 9 (8) ◽  
pp. 1610
Author(s):  
Christian Klotz ◽  
Elke Radam ◽  
Sebastian Rausch ◽  
Petra Gosten-Heinrich ◽  
Toni Aebischer
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Gastrointestinal Disease ◽  
Single Copy ◽  
Marker Genes ◽  
Small Rodent ◽  
Analytical Sensitivity ◽  
Single Copy Gene ◽  
Wild Rodents ◽  
Copy Gene

Giardiasis in humans is a gastrointestinal disease transmitted by the potentially zoonotic Giardia duodenalis genotypes (assemblages) A and B. Small wild rodents such as mice and voles are discussed as potential reservoirs for G. duodenalis but are predominantly populated by the two rodent species Giardia microti and Giardia muris. Currently, the detection of zoonotic and non-zoonotic Giardia species and genotypes in these animals relies on cumbersome PCR and sequencing approaches of genetic marker genes. This hampers the risk assessment of potential zoonotic Giardia transmissions by these animals. Here, we provide a workflow based on newly developed real-time PCR schemes targeting the small ribosomal RNA multi-copy gene locus to distinguish G. muris, G. microti and G. duodenalis infections. For the identification of potentially zoonotic G. duodenalis assemblage types A and B, an established protocol targeting the single-copy gene 4E1-HP was used. The assays were specific for the distinct Giardia species or genotypes and revealed an analytical sensitivity of approximately one or below genome equivalent for the multi-copy gene and of about 10 genome equivalents for the single-copy gene. Retesting a biobank of small rodent samples confirmed the specificity. It further identified the underlying Giardia species in four out of 11 samples that could not be typed before by PCR and sequencing. The newly developed workflow has the potential to facilitate the detection of potentially zoonotic and non-zoonotic Giardia species in wild rodents.

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