Detection of a single copy gene on a mitotic metaphase chromosome by fluorescence in situ hybridization (FISH) in the sawfly,Athalia rosae (Hymenoptera)

2001 ◽  
Vol 49 (1) ◽  
pp. 34-40 ◽  
Author(s):  
Kouhei Matsumoto ◽  
Daisuke S. Yamamoto ◽  
Megumi Sumitani ◽  
Jae Min Lee ◽  
Masatsugu Hatakeyama ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Metaphase Chromosome ◽  
Single Copy ◽  
Mitotic Metaphase ◽  
Single Copy Gene ◽  
Athalia Rosae ◽  
Mitotic Metaphase Chromosome ◽  
Copy Gene

scholarly journals Single-copy Gene Detection Using Branched DNA (bDNA) In Situ Hybridization

2001 ◽  
Vol 49 (5) ◽  
pp. 603-611 ◽  
Author(s):  
Audrey N. Player ◽  
Lu-Ping Shen ◽  
Daryn Kenny ◽  
Vincent P. Antao ◽  
Janice A. Kolberg
Keyword(s):  
In Situ Hybridization ◽  
Single Copy ◽  
Single Copy Gene ◽  
Gene Detection ◽  
Branched Dna ◽  
Copy Gene

Detection of a single-copy gene on plant chromosomes by in situ hybridization

1988 ◽  
Vol 211 (1) ◽  
pp. 143-147 ◽  
Author(s):  
Pung-Ling Huang ◽  
Klaus Hahlbrock ◽  
Imre E. Somssich
Keyword(s):  
In Situ Hybridization ◽  
Single Copy ◽  
Single Copy Gene ◽  
Plant Chromosomes ◽  
Copy Gene

Fluorescence in situ hybridization on plant extended chromatin DNA fibers for single-copy and repetitive DNA sequences

2011 ◽  
Vol 30 (9) ◽  
pp. 1779-1786 ◽  
Author(s):  
Kun Yang ◽  
Hecui Zhang ◽  
Richard Converse ◽  
Yong Wang ◽  
Xiaoying Rong ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Single Copy ◽  
Repetitive Dna Sequences

Fluorescence in situ hybridization of single copy transgenes in rice chromosomes

2001 ◽  
Vol 37 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Pushpa Kharb ◽  
Jinjiang Dong ◽  
M. N. Islam-Faridi ◽  
David M. Stelly ◽  
Timothy C. Hall
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Single Copy ◽  
Rice Chromosomes

An evaluation of a new series of fluorescent dUTPs for fluorescence in situ hybridization.

1996 ◽  
Vol 44 (5) ◽  
pp. 525-529 ◽  
Author(s):  
J Wiegant ◽  
N Verwoerd ◽  
S Mascheretti ◽  
M Bolk ◽  
H J Tanke ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Human Lymphocytes ◽  
Single Copy ◽  
Cyanine Dye ◽  
Chromosome 1 ◽  
Nick Translation ◽  
Qualitative Comparison ◽  
Copy Dna

Synthesis of fluorochrome-modified deoxyribonucleotides has been carried out mostly by linking the fluorochrome molecule to the C-5 position of dUTP via an allylamine spacer, similar to the modification of allylamine-dUTP with the haptens biotin and digoxigenin. Recently, a new series of fluorescent nucleotides has been prepared by using an alkynyl bridge between the uracil moiety and the fluorochrome. Here we report the qualitative and quantitative analysis of fluorescence in situ hybridization results obtained on interphase cells and chromosomes with a variety of highly repetitive and single-copy DNA probes that were modified by nick translation with such alkynyl dUTPs. A qualitative comparison was made of the alkynyl dUTPs conjugated to the fluorochromes fluorescein, the cyanine dye Cy3, tetramethylrhodamine, Lissamine and Texas Red. With the exception of tetramethylrhodamine, all fluorochromes performed satisfactorily. The cyanine dye Cy3 provided the highest sensitivity, i.e., cosmid and YAC probes could easily be visualized by conventional fluorescence microscopy. In a quantitative assay, different nick translation conditions were tested using a human chromosome 1 satellite III probe (pUC1.77) and alkynyl dUTPs labeled with fluorescein and Cy3. Using these two nucleotides, FISH signal intensities on interphase nuclei from human lymphocytes were quantitated by digital imaging microscopy. The strongest signals were obtained when during nick translation the ratio between dTTP and fluorescein-dUTP or Cy3-dUTP was 1:5.

Sign in / Sign up

Export Citation Format

Share Document