The in vitro synthesis and characteristics of ribosomal RNA in imaginal discs of Drosophila melanogaster

1971 ◽  
Vol 110 (3) ◽  
pp. 245-262 ◽  
Author(s):  
William H. Petri ◽  
James W. Fristrom ◽  
Dan J. Stewart ◽  
E. W. Hanly
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Imaginal Discs ◽  
In Vitro Synthesis

scholarly journals DIFFERENTIATION OF DROSOPHILA CELLS LACKING RIBOSOMAL DNA, IN VITRO

Genetics ◽  
1973 ◽  
Vol 73 (3) ◽  
pp. 429-434
Author(s):  
J James Donady ◽  
R L Seecof ◽  
M A Fox
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Ribosomal Dna ◽  
Rna Synthesis ◽  
Wild Type ◽  
Drosophila Cells

ABSTRACT Drosophila melanogaster embryos that lacked ribosomal DNA were obtained from appropriate crosses. Cells were taken from such embryos before overt differentiation took place and were cultured in vitro. These cells differentiated into neurons and myocytes with the same success as did wild-type controls. Therefore, ribosomal RNA synthesis is not necessary for the differentiation of neurons and myocytes in vitro.

Oligo(A)-stimulated Tetrahymena rDNA synthesis in vitro

1981 ◽  
Vol 59 (6) ◽  
pp. 396-403 ◽  
Author(s):  
Peter R. Ganz ◽  
Gyorgy B. Kiss ◽  
Ronald E. Pearlman
Keyword(s):  
Rna Polymerase ◽  
Dna Synthesis ◽  
Dna Polymerase ◽  
Ribosomal Rna ◽  
Replication Proteins ◽  
General Applicability ◽  
In Vitro Synthesis ◽  
Restriction Fragments ◽  
Double Stranded Dna

The synthesis of Tetrahymena rDNA has been examined using purified DNA polymerase and partially purified preparations of homologous replication enzymes (fraction IV). DNA synthesis with purified DNA polymerase alone was less than that with fraction IV enzymes. This suggested that there were additional factors in fraction IV other than DNA polymerase which contributed to or enhanced rDNA synthesis in vitro. Neither hybridization of rDNA with Tetrahymena ribosomal RNA nor preincubation of rDNA with homologous or heterologous RNA polymerase served to stimulate in vitro synthesis by fraction IV enzymes. However, when rDNA was hybridized with oligoriboadenylate, DNA synthesis using fraction IV was stimulated approximately 4- to 4.5-fold over 150 min of incubation, relative to a similarly treated but unhybridized rDNA control. Using oligoriboadenylate-hybridized EcoR1 and HindIII restriction fragments of rDNA to localize the synthesis most of the in vitro synthesis occurred within a 2.4 × 106 Mr fragment encompassing the centre of the rDNA molecule. The approach of hybridizing a synthetic homooligoribonucleotide primer to double-stranded DNA should prove to be of general applicability in designing similar template–primers in other systems for the purpose of isolating replication proteins.

The in vitro Synthesis of Ribosomal RNA

1970 ◽  
Vol 35 (0) ◽  
pp. 415-418 ◽  
Author(s):  
A. Travers ◽  
R. Kamen ◽  
M. Cashel
Keyword(s):  
Ribosomal Rna ◽  
In Vitro Synthesis

In vitro synthesis of 16S ribosomal RNA containing single base changes and assembly into a functional 30S ribosome

Biochemistry ◽  
1987 ◽  
Vol 26 (8) ◽  
pp. 2353-2364 ◽  
Author(s):  
W. Krzyzosiak ◽  
R. Denman ◽  
K. Nurse ◽  
W. Hellmann ◽  
M. Boublik ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
In Vitro Synthesis ◽  
Single Base

scholarly journals MAINTENANCE OF IMAGINAL DISCS OF DROSOPHILA MELANOGASTER IN CHEMICALLY DEFINED MEDIA

1969 ◽  
Vol 41 (3) ◽  
pp. 876-885 ◽  
Author(s):  
James A. Robb
Keyword(s):  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Synthetic Medium ◽  
Sodium Concentration ◽  
Imaginal Discs ◽  
Magnesium Concentration ◽  
Insoluble Protein ◽  
Total Rna ◽  
Phosphate Buffered Saline

A phosphate-buffered saline and a chemically defined synthetic medium for in vitro maintenance of imaginal discs of Drosophila melanogaster were developed. The composition of the chemically defined medium was varied in order to optimize the incorporation of tritiated uridine into RNA and tritiated amino acids into acid-insoluble protein. The optimal ranges obtained were: pH, 6.75–7.35; osmolarity, 285–345 milliosmoles/liter; sodium concentration, 40–60 mM/liter; potassium concentration, 40–60 mM/liter; magnesium concentration, 0.5–3.5 mM/liter; calcium concentration, 0.3–1.5 mM/liter; and inorganic phosphate concentration, 1.5–4.0 mM/liter. The phosphate-buffered saline is superior to a commonly used insect Ringer solution in maintaining total RNA and acid-insoluble protein synthesis in culture. The chemically defined synthetic medium permits linear total RNA and acid-insoluble protein synthesis for more than 48 hr, DNA synthesis for several hours, normal differentiation to occur after 74 hr in vitro, and trypsinization of imaginal discs into single cell suspensions without developmental damage.

In vitro synthesis of Escherichia coli ribosomal RNA

1973 ◽  
Vol 75 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Linda S. Birnbaum ◽  
Sam Kaplan
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Rna ◽  
In Vitro Synthesis

scholarly journals In Vitro Synthesis of Ribosomal RNA by Bacillus subtilis RNA Polymerase

1972 ◽  
Vol 69 (2) ◽  
pp. 407-411 ◽  
Author(s):  
C. Hussey ◽  
J. Pero ◽  
R. G. Shorenstein ◽  
R. Losick
Keyword(s):  
Bacillus Subtilis ◽  
Rna Polymerase ◽  
Ribosomal Rna ◽  
In Vitro Synthesis
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