Isolation and characterization of a gene from Solanum tuberosum encoding patatin, the major storage protein of potato tubers

1986 ◽  
Vol 203 (2) ◽  
pp. 214-220 ◽  
Author(s):  
Sabine Rosahl ◽  
Renate Schmidt ◽  
Jeff Schell ◽  
Lothar Willmitzer
Keyword(s):  
Solanum Tuberosum ◽  
Storage Protein ◽  
Potato Tubers ◽  
Isolation And Characterization ◽  
Major Storage Protein

scholarly journals The isolation and characterization of trigalactosyl diglyceride from potato tubers

1969 ◽  
Vol 115 (2) ◽  
pp. 335-339 ◽  
Author(s):  
T. Galliard
Keyword(s):  
Solanum Tuberosum ◽  
Total Lipids ◽  
Potato Tubers ◽  
Chromatographic Methods ◽  
Isolation And Characterization ◽  
Terminal Galactose ◽  
Intact Lipid

Trigalactosyl diglyceride has been isolated from tubers of potato (Solanum tuberosum) by a combination of chromatographic methods. This galactolipid, which constitutes approximately 1% by weight of the total lipids, was characterized by analysis of the intact lipid and its deacylation product. The fatty acids:glycerol:galactose molar proportions were shown to be close to 2:1:3. Evidence was obtained that suggests that trigalactosyl diglyceride is a higher homologue of mono- and di-galactosyl diglycerides and contains an additional d-galactopyranosyl moiety that is linked α-(1→6) to the terminal galactose unit of digalactosyl diglyceride.

Isolation and characterization of blackspot pigments from potato tubers

1996 ◽  
Vol 42 (4) ◽  
pp. 941-947 ◽  
Author(s):  
Lucas H. Stevens ◽  
Evert Davelaar
Keyword(s):  
Potato Tubers ◽  
Isolation And Characterization

scholarly journals Characterization of Streptomyces scabies isolates from potato tubers its pathogenic variations in Rawalpindi District

2014 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
S. Shazia ◽  
F. Naz ◽  
A. Rauf ◽  
M. Inam-ul-Haq ◽  
S. Bushra
Keyword(s):  
Solanum Tuberosum L ◽  
Economic Loss ◽  
Streptomyces Scabies ◽  
Potato Tubers ◽  
Yield And Quality ◽  
Isolation And Characterization ◽  
Pathogenic Variation ◽  
Important Disease

Potato (Solanum tuberosum L.) has various biotic constraints in its production due to pest attack. Among these, common scab caused by streptomyces scabies is an important disease in potato which causes economic loss with respect to plant yield and quality of tubers. The present study was conducted to determine the pathogenicity, pathogenic variation, characterization of morphological, physiological and biochemical aspects of Streptomyces associated with potato tubers grown in Rawalpindi district. Severity data and pathogenic variation of disease was studied by using different isolation and characterization techniques.

scholarly journals Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector

1988 ◽  
Vol 252 (1) ◽  
pp. 199-206 ◽  
Author(s):  
D L Andrews ◽  
B Beames ◽  
M D Summers ◽  
W D Park
Keyword(s):  
Solanum Tuberosum ◽  
Cdna Clone ◽  
Storage Protein ◽  
Enzymic Activity ◽  
Total Soluble Protein ◽  
Baculovirus Vector ◽  
Tuber Protein ◽  
Lipid Acyl Hydrolase ◽  
Baculovirus System

Patatin is a family of glycoproteins that accounts for 30-40% of the total soluble protein in potato (Solanum tuberosum) tubers. This protein has been reported not only to serve as a storage protein, but also to exhibit enzymic activity. By using a baculovirus system to express protein from the patatin cDNA clone pGM01, it was unambiguously shown that the patatin coded by this DNA has lipid acyl hydrolase and acyltransferase activities. The enzyme is active with phospholipids, monoacylglycerols and p-nitrophenyl esters, moderately active with galactolipids, but is apparently inactive with di- and tri-acylglycerols.

Isolation and characterization of an expressed napin gene fromBrassica rapa

1991 ◽  
Vol 1 (4) ◽  
pp. 209-219 ◽  
Author(s):  
Jean C. Kridl ◽  
David W. McCarter ◽  
Ronald E. Rose ◽  
Donna E. Scherer ◽  
Deborah S. Knutzon ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Storage Protein ◽  
Protein Gene ◽  
Coding Region ◽  
Coding Regions ◽  
Endogenous Expression ◽  
Isolation And Characterization ◽  
Glucuronidase Reporter ◽  
Transgenic Rapeseed

AbstractAn expressed napin storage protein gene fromBrassica rapa, BcNA1, has been cloned and sequenced. The gene is a member of a family of four to seven napin genes inB. rapaand is highly expressed in developing seeds. An expression cassette containing the DNA flanking the napin coding region of BcNA1 has been engineered and demonstrated to function appropriately, as compared with the gene's endogenous expression, in transgenic rapeseed using the β-glucuronidase reporter gene. TheB. rapaBcNA1 gene and aB. napusnapin gene, gNa, share extremely high nucleotide homology not only throughout their coding regions, but over a DNA locus comprising 4.3 kb. We suggest the gNa gene was contributed by the originalB. rapaprogenitor of the amphidiploidB. napus.

Biochemical Characterization of Amandin, the Major Storage Protein in Almond (Prunus dulcisL.)

2002 ◽  
Vol 50 (15) ◽  
pp. 4333-4341 ◽  
Author(s):  
Shridhar K. Sathe ◽  
Walter J. Wolf ◽  
Kenneth H. Roux ◽  
Suzanne S. Teuber ◽  
Mahesh Venkatachalam ◽  
...  
Keyword(s):  
Storage Protein ◽  
Biochemical Characterization ◽  
Major Storage Protein

scholarly journals Isolation and characterization of StERF transcription factor genes from potato (Solanum tuberosum L.)

2015 ◽  
Vol 338 (4) ◽  
pp. 219-226 ◽  
Author(s):  
Zemin Wang ◽  
Ning Zhang ◽  
Xiangyan Zhou ◽  
Qiang Fan ◽  
Huaijun Si ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Isolation And Characterization ◽  
Transcription Factor Genes
Sign in / Sign up

Export Citation Format

Share Document