Plant-inducible recombination between the 25 bp border sequences of T-DNA in Agrobacterium tumefaciens

1986 ◽  
Vol 204 (3) ◽  
pp. 374-382 ◽  
Author(s):  
Yasumori Machida ◽  
Shoji Usami ◽  
Akiko Yamamoto ◽  
Yasuo Niwa ◽  
Itaru Takebe
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Border Sequences

scholarly journals A T-DNA from the Agrobacterium tumefaciens Limited-Host-Range Strain AB2/73 Contains a Single Oncogene

1998 ◽  
Vol 11 (5) ◽  
pp. 335-342 ◽  
Author(s):  
Léon Otten ◽  
Julien Schmidt
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Host Range ◽  
Dna Sequence ◽  
Ti Plasmid ◽  
Dna Homology ◽  
Agrobacterium Vitis ◽  
E Gene ◽  
Limited Host Range ◽  
Border Sequences ◽  
Border Sequence

Agrobacterium tumefaciens strain AB2/73 isolated from Lippia canescens has been described as a limited-host-range strain. Its tumor-inducing (Ti) plasmid has been found to lack DNA homology to known T-DNAs (L. Unger, S. F. Ziegler, G. A. Huffman, V. C. Knauf, R. Peet, L. W. Moore, M. P. Gordon, and E. W. Nester. J. Bacteriol. 164:723–730, 1985). We have isolated a T-DNA from AB2/73 by using a heterologous border sequence as a probe. The AB2/73 T-DNA sequence (3,504 bp) is flanked by canonical border sequences, has no detectable DNA homology with other T-DNAs, and contains only two genes: lsn ( Lippia strain nopaline synthaselike gene) and lso ( Lippia strain oncogene). The lso gene induces nondif-ferentiating tumors on a limited number of hosts when transferred by a Ti plasmid from a wide-host-range strain. Part of the predicted Lso protein is weakly homologous to other Agrobacterium oncoproteins encoded by rolB, rolBTR, orf13, gene e, gene 5, and gene 3′. A 28-kb fragment corresponding to the virA to virE region was cloned by using a heterologous vir fragment as probe. The AB2/73 vir region is homologous to most of the C58 virulence region; however, the virA gene is most related to the virA gene of the Agrobacterium vitis limited-host-range strain Ag162.

Genetic transformation of Ginkgo biloba by Agrobacterium tumefaciens

2000 ◽  
Vol 108 (4) ◽  
pp. 413-419
Author(s):  
Patricia Dupré ◽  
Jerôme Lacoux ◽  
Godfrey Neutelings ◽  
Dominique Mattar-Laurain ◽  
Marc-André Fliniaux ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Ginkgo Biloba
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