Cloning and expression of the Rhodospirillum rubrum ribulosebisphosphate carboxylase gene in E. coli

1984 ◽  
Vol 193 (2) ◽  
pp. 214-219 ◽  
Author(s):  
Christopher R. Somerville ◽  
Shauna C. Somerville
Keyword(s):  
Rhodospirillum Rubrum ◽  
Cloning And Expression ◽  
E Coli ◽  
Ribulosebisphosphate Carboxylase

Rapid E. coli-based cloning and expression system for production of recombinant proteins

2014 ◽  
Vol 185 ◽  
pp. S70
Author(s):  
Boguslaw Lupa ◽  
Krzysztof Stawujak ◽  
Igor Rozanski ◽  
Justyna Stec-Niemczyk
Keyword(s):  
Recombinant Proteins ◽  
Expression System ◽  
Cloning And Expression ◽  
E Coli

scholarly journals Escherichia coli as a genetic tool

1985 ◽  
Vol 95 (3) ◽  
pp. 611-618
Author(s):  
Naomi Datta
Keyword(s):  
Escherichia Coli ◽  
Academic Research ◽  
Genetically Engineered ◽  
Cloning And Expression ◽  
Biological Research ◽  
New Techniques ◽  
E Coli ◽  
Genetic Tool ◽  
The Past ◽  
Made In

SUMMARYThe study of Escherichia coli and its plasmids and bacteriophages has provided a vast body of genetical information, much of it relevant to the whole of biology. This was true even before the development of the new techniques, for cloning and analysing DNA, that have revolutionized biological research during the past decade. Thousands of millions of dollars are now invested in industrial uses of these techniques, which all depend on discoveries made in the course of academic research on E. coli. Much of the background of knowledge necessary for the cloning and expression of genetically engineered information, as well as the techniques themselves, came from work with this organism.

Cloning and expression of Xylella fastidiosa antigens in Escherichia coli and Erwinia stewartii

1989 ◽  
Vol 35 (4) ◽  
pp. 487-491 ◽  
Author(s):  
Paul H. Goodwin
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Genomic Dna ◽  
Xylella Fastidiosa ◽  
Gene Bank ◽  
Cloning And Expression ◽  
Western Blots ◽  
E Coli ◽  
Cosmid Vector ◽  
Erwinia Stewartii

Xylella fastidiosa DNA, partially digested with Sau3A, was ligated into the cosmid vector, pUCD615. Approximately 4500 ampicillin-resistant Escherichia coli colonies were obtained. The frequency of complementation of leucine auxotrophy in transfected E. coli indicated that the cosmid gene bank was representative of X. fastidiosa genomic DNA. Colonies were lysed directly onto nitrocellulose membranes using a thermo-inducible λ lysogen and screened for expression of X. fastidiosa antigens. Approximately 16.5% of a random sample of clones were found to express X. fastidiosa antigens as determined by Western blots. These proteins comigrated with proteins of X. fastidiosa and ranged in molecular weight from 10 000 to 160 000. Conjugation of several of the plasmids into Erwinia stewartii resulted in expression of the similar molecular weight cloned proteins with similar levels of expression as in E. coli.Key words: Xylella fastidiosa, Pierce's disease, immunological clone screening, thermo-inducible lysogeny.

Cloning and expression of α-amylase in E. coli: genesis of a superior biocatalyst for substrate-specific MFC

2019 ◽  
Vol 16 (4) ◽  
pp. 309-316 ◽  
Author(s):  
Arpita Nandy ◽  
Samir Jana ◽  
Moumita Khamrai ◽  
Vikash Kumar ◽  
Shritama Mukherjee ◽  
...  
Keyword(s):  
Cloning And Expression ◽  
E Coli

Cloning and expression of DiT33 from Dirofilaria immitis: a specific and early marker of heartworm infection

Parasitology ◽  
1996 ◽  
Vol 112 (3) ◽  
pp. 331-338 ◽  
Author(s):  
X. Q. Hong ◽  
J. Santiago Mejia ◽  
S. Kumar ◽  
F. B. Perler ◽  
C. K. S. Carlow
Keyword(s):  
Dirofilaria Immitis ◽  
Cloning And Expression ◽  
E Coli ◽  
Spliced Leader ◽  
Isolation And Characterization ◽  
The Family ◽  
Heartworm Infection ◽  
High Level ◽  
Control And Management

SUMMARYDirofilaria immitis is an important filarial parasite of dogs and cats, and a useful model for human filariasis. Current diagnostic tests for heartworm infection in animals rely on the presence of fecund female worms (usually found 6·5 months post-infection or later) and therefore fail to detect pre-patent infections. Putative pepsin inhibitors from 2 filarial parasites of humans namely Onchocerca volvulus (Ov33, Oc3.6, OvDSB) and Brugia malayi (Bm33), have been shown to be useful in diagnosis of onchocerciasis and lymphatic filariasis, respectively. Previous studies have suggested that a homologue exists in D. immitis (DiT33), which may have potential in diagnosis of heartworm infection. In this study, the isolation and characterization of a cDNA clone encoding DiT33 is described.‡ This cDNA contains 12 bases of the nematode-specific 22 nucleotide spliced leader sequence and encodes a 26·4 kDa-protein with a high level of similarity (87–89%) to other filarial members of the family. DJT33 was over-expressed in E. coli as a fusion with the maltose-binding protein and serological analysis was performed using a panel of clinically defined dog sera. The findings of this study indicate that DiT33 is a promising antigen for the early detection of D. immitis and may be a valuable tool in the control and management of heartworm infection.

Cloning and expression of l-asparaginase from E. coli in eukaryotic expression system

2015 ◽  
Vol 102 ◽  
pp. 14-17 ◽  
Author(s):  
Syed Sajitha ◽  
Jalaja Vidya ◽  
karunakaran Varsha ◽  
Parameswaran Binod ◽  
Ashok Pandey
Keyword(s):  
Expression System ◽  
Eukaryotic Expression ◽  
Cloning And Expression ◽  
E Coli ◽  
Eukaryotic Expression System

Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli

Nature ◽  
1983 ◽  
Vol 301 (5897) ◽  
pp. 214-221 ◽  
Author(s):  
Diane Pennica ◽  
William E. Holmes ◽  
William J. Kohr ◽  
Richard N. Harkins ◽  
Gordon A. Vehar ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Human Tissue ◽  
Cloning And Expression ◽  
Tissue Type ◽  
Tissue Type Plasminogen Activator ◽  
E Coli ◽  
Type Plasminogen Activator
Sign in / Sign up

Export Citation Format

Share Document