Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli

Nature ◽  
1983 ◽  
Vol 301 (5897) ◽  
pp. 214-221 ◽  
Author(s):  
Diane Pennica ◽  
William E. Holmes ◽  
William J. Kohr ◽  
Richard N. Harkins ◽  
Gordon A. Vehar ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Human Tissue ◽  
Cloning And Expression ◽  
Tissue Type ◽  
Tissue Type Plasminogen Activator ◽  
E Coli ◽  
Type Plasminogen Activator

scholarly journals Influence of fibrin and liver blood flow on the turnover and the systemic fibrinogenolytic effects of recombinant human tissue-type plasminogen activator in rabbits

Blood ◽  
1986 ◽  
Vol 67 (5) ◽  
pp. 1493-1497 ◽  
Author(s):  
H Bounameaux ◽  
JM Stassen ◽  
C Seghers ◽  
D Collen
Keyword(s):  
Blood Flow ◽  
Plasminogen Activator ◽  
Human Tissue ◽  
Femoral Vein ◽  
Liver Blood Flow ◽  
Tissue Type ◽  
Tissue Type Plasminogen Activator ◽  
Cardiovascular Failure ◽  
Type Plasminogen Activator ◽  
Impaired Liver

Abstract The influence of the presence of fibrin microclots on the systemic fibrinogenolytic effects of intravenous (IV) recombinant human tissue- type plasminogen activator (rt-PA) was studied by injection of a homogenized fibrin suspension in the femoral vein or artery in rabbits. A linear correlation (P less than .001) was found between the extent of fibrinogen breakdown and the amount of fibrin (0 to 32 mg/kg) injected just prior to the IV infusion of rt-PA at a rate of 10 micrograms/kg/min for 60 minutes. This finding suggests that the systemic activation of the fibrinolytic system observed in some patients during infusion of rt-PA may be due, at least in part, to the presence of fibrin in the vascular bed. The effect of blood flow in the liver on the turnover of rt-PA was measured in rabbits after ligation of the hepatic artery and monitoring of the blood flow in the portal vein with a peristaltic pump. The half-life (t1/2) of rt-PA in plasma was inversely correlated with the logarithm of the rate of the liver blood flow. A doubling of the plasma t1/2 of rt-PA was observed after an eightfold reduction of the liver blood flow, suggesting that delayed clearance of rt-PA may occur in patients with severe cardiovascular failure and impaired liver blood flow.

Low Doses of Recombinant Human Tissue-Type Plasminogen Activator for Local Thrombolysis in Peripheral Arteries

Radiology ◽  
1988 ◽  
Vol 168 (3) ◽  
pp. 877-877 ◽  
Author(s):  
Renate Koppensteiner ◽  
Erich Minar ◽  
Ramazanali Ahmadi ◽  
Michael Jung ◽  
Herbert Ehringer
Keyword(s):  
Plasminogen Activator ◽  
Human Tissue ◽  
Tissue Type ◽  
Low Doses ◽  
Peripheral Arteries ◽  
Tissue Type Plasminogen Activator ◽  
Local Thrombolysis ◽  
Type Plasminogen Activator
Sign in / Sign up

Export Citation Format

Share Document