Size and organization of a multigene family encoding phaseolin, the major seed storage protein of Phaseolus vulgaris L.

1984 ◽  
Vol 198 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Duncan R. Talbot ◽  
Michael J. Adang ◽  
Jerry L. Slightom ◽  
Timothy C. Hall
Keyword(s):  
Phaseolus Vulgaris ◽  
Multigene Family ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
Phaseolus Vulgaris L

scholarly journals Variation in the Accumulation of Seed Storage Protein Among Genotypes of Phaseolus vulgaris (L.)

1980 ◽  
Vol 65 (4) ◽  
pp. 627-630 ◽  
Author(s):  
Martha A. Mutschler ◽  
Frederick A. Bliss ◽  
Timothy C. Hall
Keyword(s):  
Phaseolus Vulgaris ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
Phaseolus Vulgaris L

Phaseolin nucleotide sequence diversity in Phaseolus. I. Intraspecific diversity in Phaseolus vulgaris

Genome ◽  
1994 ◽  
Vol 37 (5) ◽  
pp. 751-757 ◽  
Author(s):  
James A. Kami ◽  
Paul Gepts
Keyword(s):  
Phaseolus Vulgaris ◽  
Dna Sequences ◽  
Storage Protein ◽  
Gene Pool ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
Direct Repeat ◽  
Intraspecific Diversity ◽  
Cdna Clones ◽  
Gene Pools

Most information about the molecular biology of phaseolin, the major seed storage protein in Phaseolus vulgaris, has been obtained from the T-type phaseolin, which is characteristic of the Andean gene pool of the species. In the work reported here, two cDNA clones for the S-type phaseolin representing the other major, Middle American gene pool were isolated and sequenced. Analysis of the DNA sequences revealed the presence of two subtypes of S phaseolin, α and β, depending on the presence or absence, respectively, of a 27-bp direct repeat. These are similar to the α- and β-phaseolin subtypes found in the Andean, T phaseolin; however, the additional 15-bp direct repeat also found in the T α-phaseolin gene type was apparently absent from the S α-phaseolin genes. The overall sequence identity was greater between the α or β subtypes of different gene pools than between the a or p subtypes within gene pools. This implies that the gene subtypes were formed prior to the formation of the two major gene pools of P. vulgaris. Analysis of the putative amino acid sequence revealed that both the 'Sanilac' phaseolin subtypes contained an additional methionine, however, not at the same site. This opens the possibility of increasing the nutritionally limiting methionine level in phaseolin either through protein engineering or by screening accessions for recombinant phaseolin sequences that combine both substitutions.Key words: seed storage protein, multigene family, direct repeat.

scholarly journals Molecular cytogenetical and biochemical studies on some Lupinus species

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Hoda B. M. Ali ◽  
Samy A. A. Heiba
Keyword(s):  
Related Species ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Lupinus Albus ◽  
Seed Storage ◽  
Rrna Genes ◽  
Large Chromosome ◽  
45S Rdna ◽  
Lupinus Polyphyllus ◽  
Electrophoretic Patterns

Abstract Background Lupins are cultivated as human consumption grains and forage legumes. The chromosomes of lupins are too small to be karyotyped by conventional techniques, because they reveal a general lack of distinctive cytological features. In the current study, Fluorescence in situ Hybridization (FISH) was used to locate 5S and 45S ribosomal gene sites on the chromosomes of Lupinus albus ssp albus, Lupinus albus ssp graecus, Lupnus termis (all with 2n = 50), and Lupinus polyphyllus lindl var. polyphyllus (2n = 48), FISH together with seed storage protein electrophoretic patterns were used to find out the relationship among these species. Results The double-target FISH on the chromosomes of the studied species with rDNA probes revealed that the two types of rRNA genes are located on different chromosomes. The detected loci of rRNA genes partially reflected the taxonomical similarity among the two Lupinus albus subspecies and L. termis. Lupinus polyphyllus lindl var. polyphyllus was exception by having unique large chromosome mostly is covered by one signal of 45S rDNA, whereas its homologous chromosome seems to be normal-sized and have the other 45S rDNA locus. The similarity matrix among the Lupinus species as computed according to Jaccardʼs Coefficient from the SDS-PAGE, showed that L. albus ssp. Albus and L. albus ssp. Graecus are the most similar species (~ 97%), and then comes L. termis, and L. polyphyllus lindl var. polyphylus has been placed in separate clade and still the most related species to it among the studied species is L. termis (~ 70%). Conclusion It could be postulated from FISH and seed storage protein electrophoretic patterns that the relationships among the studied species is as follows, Lupinus albus ssp albus, is the most related species to Lupinus albus ssp graecus then comes Lupnus termis and Lupinus polyphyllus lindl var. polyphyllus at a distal position.

Ana o 1, a cashew (Anacardium occidental) allergen of the vicilin seed storage protein family

2002 ◽  
Vol 110 (1) ◽  
pp. 160-166 ◽  
Author(s):  
Fang Wang ◽  
Jason M. Robotham ◽  
Suzanne S. Teuber ◽  
Pallavi Tawde ◽  
Shridhar K. Sathe ◽  
...  
Keyword(s):  
Storage Protein ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
Protein Family

Highly Recombinogenic Regions at Seed Storage Protein Loci on Chromosome 1DS of Aegilops tauschii, the D-Genome Donor of Wheat

Genetics ◽  
2000 ◽  
Vol 155 (1) ◽  
pp. 361-367 ◽  
Author(s):  
Wolfgang Spielmeyer ◽  
Odile Moullet ◽  
André Laroche ◽  
Evans S Lagudah
Keyword(s):  
Genetic Distance ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Aegilops Tauschii ◽  
Bac Library ◽  
Seed Storage ◽  
D Genome ◽  
Rflp Map ◽  
Genome Donor ◽  
The Relationship

Abstract A detailed RFLP map was constructed of the distal end of the short arm of chromosome 1D of Aegilops tauschii, the diploid D-genome donor species of hexaploid wheat. Ae. tauschii was used to overcome some of the limitations commonly associated with molecular studies of wheat such as low levels of DNA polymorphism. Detection of multiple loci by most RFLP probes suggests that gene duplication events have occurred throughout this chromosomal region. Large DNA fragments isolated from a BAC library of Ae. tauschii were used to determine the relationship between physical and genetic distance at seed storage protein loci located at the distal end of chromosome 1DS. Highly recombinogenic regions were identified where the ratio of physical to genetic distance was estimated to be <20 kb/cM. These results are discussed in relation to the genome-wide estimate of the relationship between physical and genetic distance.

A transient assay for promoter activity of wheat seed storage protein genes and other genes expressed in developing endosperm

Plant Science ◽  
1994 ◽  
Vol 102 (1) ◽  
pp. 69-80 ◽  
Author(s):  
Ann E. Blechl ◽  
Gale F. Lorens ◽  
Frank C. Greene ◽  
Bruce E. Mackey ◽  
Olin D. Anderson
Keyword(s):  
Storage Protein ◽  
Promoter Activity ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
Transient Assay ◽  
Wheat Seed ◽  
Storage Protein Genes

Evolution of 2S albumin seed storage protein genes in the Brassicaceae

1999 ◽  
Vol 27 (3) ◽  
pp. 223-234 ◽  
Author(s):  
Kim Boutilier ◽  
Jiro Hattori ◽  
Bernard R. Baum ◽  
Brian L. Miki
Keyword(s):  
Storage Protein ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
2S Albumin ◽  
Storage Protein Genes

Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 1018-1021 ◽  
Author(s):  
M. Nenno ◽  
K. Schumann ◽  
W. Nagl
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Polytene Chromosomes ◽  
Seed Storage ◽  
Phaseolus Coccineus ◽  
Rna Genes

This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.Key words: plant, Leguminosae, ribosomal RNA genes, seed storage protein genes, protease.

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