The role of auxin in hairy root induction

1987 ◽  
Vol 208 (3) ◽  
pp. 457-463 ◽  
Author(s):  
Maura Cardarelli ◽  
Laura Spanò ◽  
Domenico Mariotti ◽  
Maria Luisa Mauro ◽  
Marie Anne Van Sluys ◽  
...  
Keyword(s):  
Hairy Root ◽  
Root Induction ◽  
Hairy Root Induction

scholarly journals Hairy root induction from hypocotyl segments of groundnut (Arachis hypogaea L.)

2007 ◽  
Vol 6 (15) ◽  
pp. 1817-1820 ◽  
Author(s):  
KARTHIKEYAN A ◽  
PALANIVEL S PARVATHY S ◽  
BHAKYA RAJ R
Keyword(s):  
Arachis Hypogaea ◽  
Hairy Root ◽  
Root Induction ◽  
Arachis Hypogaea L ◽  
Hairy Root Induction ◽  
Hypocotyl Segments

scholarly journals Hairy root induction and benzylisoquinoline alkaloid production in Macleaya cordata

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Peng Huang ◽  
Liqiong Xia ◽  
Wei Liu ◽  
Ruolan Jiang ◽  
Xiubin Liu ◽  
...  
Keyword(s):  
Hairy Root ◽  
Root Induction ◽  
Alkaloid Production ◽  
Benzylisoquinoline Alkaloid ◽  
Macleaya Cordata ◽  
Hairy Root Induction

Technology and Application of Hairy Root Culture in Monocotyledons

2021 ◽  
Vol 07 ◽  
Author(s):  
Chang-Qi Hao ◽  
Shuai-Run Wang ◽  
Yi Wang ◽  
Xin-Yi Hou ◽  
Ya-Xuan Jiang ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Food Crops ◽  
Root Induction ◽  
Hairy Root Induction ◽  
Selection Of

Background: Hairy root culture has been widely used in the production of metabolites in dicotyledons, and a large number of food crops and medicinal plants in monocotyledons need to be developed, but there are many difficulties in the induction of hairy roots in monocotyledons. The purpose of this paper is to introduce the inducing methods, influencing factors and application of hairy roots in monocotyledons, and to promote the development of hairy root system in monocotyledons. Methods: The mechanism of action of Agrobacterium rhizogenes and the current situation of hairy root induction, induction methods and influencing factors of monocotyledons were summarized so as to provide convenience for efficient acquisition of hairy root of monocotyledons. Results: Monocotyledons are not easy to produce phenols, cells are prone to lignification, adverse differentiation and selective response to Agrobacterium rhizogenes strains. It is proposed that before induction, plant varieties and explants should be selected, and different infection strains should be screened. In the process of hairy root induction, exogenous inducers such as acetosyringone can be added. Although these factors can provide some help for the induction of hairy roots in monocotyledons, we still need to pay attention to the disadvantages of monocotyledons from dicotyledons at the cellular level. Conclusion: A large number of food crops and medicinal plants are monocotyledons. Hairy root culture can be used to help the breeding and production of medicinal substances. Therefore, it is necessary to pay attention to the selection of varieties and explants, the selection of Agrobacterium rhizogenes and the addition of acetosyringone in the process of hairy root induction so as to improve the production efficiency and facilitate the development and utilization of monocotyledons.

scholarly journals Influences of various factors on hairy root induction in Agastache foeniculum (Pursh) Kuntze

2016 ◽  
Vol 107 (1) ◽  
pp. 45 ◽  
Author(s):  
Elnaz NOUROZI ◽  
Bahman HOSSEINI ◽  
Abbas HASSANI
Keyword(s):  
Growth Rate ◽  
Hairy Root ◽  
Hairy Roots ◽  
Acid Content ◽  
Rosmarinic Acid ◽  
Culture Media ◽  
Root Induction ◽  
Transformed Roots ◽  
Inoculation Methods ◽  
Hairy Root Induction

<em>Agrobacterium rhizogenes</em> is known as a natural tool of genetic engineering in many plant species. For the first time, hairy root induction in <em>Agastache foeniculum</em> using <em>A. rhizogenes</em>,<em> </em>rosmarinic acid content<em> </em>and the effect of different culture media and inoculation methods on hairy root growth rate were investigated. Hairy root culture of <em>A. foeniculum</em> was established by inoculation of the 1-month-old leaf explant with A4 strain of <em>A. rhizogenes</em> and the effectiveness of light – dark conditions and two inoculation methods (immersion and injection) were tested. Furthermore, in immersion method, the effects of inoculation time (3, 5 and 7 min) on root induction were investigated. In the second part of the study, the hairy root culture of <em>A. foeniculum</em> was studied using different<em> </em>basal culture media (MS, 1/2 MS and B5). Rosmarinic acid content in hairy roots and non- transformed roots was analyzed using high-performance liquid chromatography (HPLC). There was no significant difference between various inoculation methods in the ability of hairy roots induction. Observations showed that percentage of hairy root induction was higher when the explants were immersed for 5 min in bacterial suspension. Light conditions displayed the highest hairy root induction rates compared with dark condition. Various culture media are different in terms of types and amounts of nutrients and have influence on growth rate. The maximum growth rate (1.61 g fr wt/50 ml) of hairy roots were obtained in 1/2 MS medium. Rosmarinic acid content in transformed roots (213.42 µg/g dry wt) was significantly higher than non-transformed roots (52.28 µg/ g dry wt).

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