In situ hybridization of ribosomal DNA labelled with 125iodine to metaphase and lampbrush chromosomes from newts

Chromosoma ◽  
1975 ◽  
Vol 50 (4) ◽  
pp. 349-369 ◽  
Author(s):  
Sally Hennen ◽  
S. Mizuno ◽  
H. C. Macgregor
1972 ◽  
Vol 54 (3) ◽  
pp. 580-591 ◽  
Author(s):  
Giuseppina Barsacchi ◽  
Joseph G. Gall

The repetitive DNA sequences of the newt, Triturus viridescens, have been studied by nucleic acid hybridization procedures. Complementary RNA was synthesized enzymatically from unfractionated newt DNA. This RNA hybridized strongly to the centromeric regions of both somatic and lampbrush chromosomes It also bound to other loci scattered along the lengths of the chromosomes The amplified ribosomal DNA in the multiple oocyte nucleoli was demonstrated by in situ hybridization


Mycorrhiza ◽  
1999 ◽  
Vol 8 (4) ◽  
pp. 203-206 ◽  
Author(s):  
Sophie Trouvelot ◽  
Diederik van Tuinen ◽  
Mohamed Hijri ◽  
V. Gianinazzi-Pearson

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 582-587 ◽  
Author(s):  
R. J. Snowdon ◽  
W. Köhler ◽  
A. Köhler

Using fluorescence in situ hybridization, we located ribosomal DNA loci on prometaphase chromosomes of the diploid species Brassica rapa and Brassica oleracea and their amphidiploid Brassica napus. Based on comparisons of chromosome morphology and hybridization patterns, we characterized the individual B. napus rDNA loci according to their presumed origins in the Brassica A and C genomes. As reported in other studies, the sum of rDNA loci observed on B. rapa (AA genome) and B. oleracea (CC genome) chromosomes was one greater than the total number of loci seen in their amphidiploid B. napus (AACC). Evidence is presented that this reduction in B. napus rDNA locus number results from the loss of the smallest A genome rDNA site in the amphidiploid.Key words: Brassica, fluorescence in situ hybridization, ribosomal DNA, rDNA.


1997 ◽  
Vol 88 (2) ◽  
pp. 158-161 ◽  
Author(s):  
Y. Ma ◽  
M. N. Islam-Faridi ◽  
C. F. Crane ◽  
Y. Ji ◽  
D. M. Stelly ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document