Visualization of ribosomal DNA loci in spore interphasic nuclei of glomalean fungi by fluorescence in situ hybridization

Mycorrhiza ◽  
1999 ◽  
Vol 8 (4) ◽  
pp. 203-206 ◽  
Author(s):  
Sophie Trouvelot ◽  
Diederik van Tuinen ◽  
Mohamed Hijri ◽  
V. Gianinazzi-Pearson
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Dna ◽  
Glomalean Fungi

Chromosomal localization and characterization of rDNA loci in the Brassica A and C genomes

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 582-587 ◽  
Author(s):  
R. J. Snowdon ◽  
W. Köhler ◽  
A. Köhler
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Dna ◽  
Diploid Species ◽  
Rdna Locus ◽  
Chromosome Morphology ◽  
Rdna Site ◽  
A Genome ◽  
Rdna Loci

Using fluorescence in situ hybridization, we located ribosomal DNA loci on prometaphase chromosomes of the diploid species Brassica rapa and Brassica oleracea and their amphidiploid Brassica napus. Based on comparisons of chromosome morphology and hybridization patterns, we characterized the individual B. napus rDNA loci according to their presumed origins in the Brassica A and C genomes. As reported in other studies, the sum of rDNA loci observed on B. rapa (AA genome) and B. oleracea (CC genome) chromosomes was one greater than the total number of loci seen in their amphidiploid B. napus (AACC). Evidence is presented that this reduction in B. napus rDNA locus number results from the loss of the smallest A genome rDNA site in the amphidiploid.Key words: Brassica, fluorescence in situ hybridization, ribosomal DNA, rDNA.

Physical localisation of repetitive DNA sequences in Alstroemeria: karyotyping of two species with species-specific and ribosomal DNA

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 652-658 ◽  
Author(s):  
Silvan A. Kamstra ◽  
Anja G. J. Kuipers ◽  
Marjo J. De Jeu ◽  
M. S. Ramanna ◽  
Evert Jacobsen
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Repetitive Dna Sequences ◽  
Metaphase Chromosomes ◽  
Rdna Sites ◽  
Species Specific

Fluorescence in situ hybridization (FISH) was used to localise two species-specific repetitive DNA sequences, A001-I and D32-13, and two highly conserved 25S and 5S rDNA sequences on the metaphase chromosomes of two species of Alstroemeria. The Chilean species, Alstroemeria aurea (2n = 16), has abundant constitutive heterochromatin, whereas the Brazilian species, Alstroemeria inodora, has hardly any heterochromatin. The A. aurea specific A001-I probe hybridized specifically to the C-band regions on all chromosomes. The FISH patterns on A. inodora chromosomes using species-specific probe D32–13 resembled the C-banding pattern and the A001-I pattern on A. aurea chromosomes. There were notable differences in number and distribution of rDNA sites between the two species. The 25S rDNA probe revealed 16 sites in A. aurea that closely colocalised with A001-I sites and 12 in A. inodora that were predominantly detected in the centromeric regions. FISH karyotypes of the two Alstroemeria species were constructed accordingly, enabling full identification of all individual chromosomes. These FISH karyotypes will be useful for monitoring the chromosomes of both Alstroemeria species in hybrids and backcross derivatives.Key words: Alstroemeria, fluorescence in situ hybridization, FISH, repetitive DNA, ribosomal DNA, karyotype.

scholarly journals Prevalence of Bacteria of Division TM7 in Human Subgingival Plaque and Their Association with Disease

2003 ◽  
Vol 69 (3) ◽  
pp. 1687-1694 ◽  
Author(s):  
Mary M. Brinig ◽  
Paul W. Lepp ◽  
Cleber C. Ouverney ◽  
Gary C. Armitage ◽  
David A. Relman
Keyword(s):  
In Situ Hybridization ◽  
Periodontal Disease ◽  
Fluorescence In Situ Hybridization ◽  
Quantitative Pcr ◽  
Ribosomal Dna ◽  
Subgingival Plaque ◽  
Real Time Quantitative Pcr ◽  
Bacterial Division ◽  
Severe Periodontitis

ABSTRACT Members of the uncultivated bacterial division TM7 have been detected in the human mouth, but little information is available regarding their prevalence and diversity at this site. Human subgingival plaque samples from healthy sites and sites exhibiting various stages of periodontal disease were analyzed for the presence of TM7 bacteria. TM7 ribosomal DNA (rDNA) was found in 96% of the samples, and it accounted for approximately 0.3%, on average, of all bacterial rDNA in the samples as determined by real-time quantitative PCR. Two new phylotypes of this division were identified, and members of the division were found to exhibit filamentous morphology by fluorescence in situ hybridization. The abundance of TM7 rDNA relative to total bacterial rDNA was higher in sites with mild periodontitis (0.54% ± 0.1%) than in either healthy sites (0.21% ± 0.05%, P < 0.01) or sites with severe periodontitis (0.29% ± 0.06%, P < 0.05). One division subgroup, the I025 phylotype, was detected in 1 of 18 healthy samples and 38 of 58 disease samples. These data suggest that this phylotype, and the TM7 bacterial division in general, may play a role in the multifactorial process leading to periodontitis.

Localization of Two Types of Ribosomal DNA Using Fluorescence in situ Hybridization in Five Wild Rose Species

2017 ◽  
Vol 25 (3) ◽  
pp. 110-117 ◽  
Author(s):  
Yoon-Jung Hwang ◽  
◽  
Yoon Ha Ju ◽  
Franklin Hinosa Mancia ◽  
Yun-Im Kang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Dna

Variability in rDNA loci in Iberian species of the genus Zabrus (Coleoptera: Carabidae) detected by fluorescence in situ hybridization

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 22-28 ◽  
Author(s):  
JF Sánchez-Gea ◽  
J Serrano ◽  
J Galián
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Number ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Dna ◽  
Ground Beetle ◽  
Morphological Characters ◽  
Rdna Sites ◽  
Rdna Loci ◽  
Numerical Variation

Fluorescence in situ hybridization (FISH) with a PCR-amplified 18S ribosomal probe was used to map rDNA loci in 19 taxa of the ground beetle genus Zabrus (2n = 47-63) from the Iberian Peninsula. A quantitative and qualitative variation has been observed among related species, subspecies, populations, and even individuals. The number of rDNA-carrying chromosomes varies from 2 to 12, and the extent of the signal from small dots to entire arms. Changes altering the number of rDNA clusters seem to be uncoupled from the variation found in the chromosome number. Mechanisms that explain the numerical variation and spreading of rDNA clusters throughout the genome within the genus Zabrus are briefly discussed. No concordance between the pattern of rDNA sites and the phylogenetic relationships as based on morphological characters has been found. Key words: Carabidae, Coleoptera, fluorescence in situ hybridization, polymorphism, ribosomal DNA, Zabrus.

Preliminary karyotype and chromosomal localization of ribosomal DNA sites in white spruce using fluorescence in situ hybridization

Genome ◽  
1993 ◽  
Vol 36 (2) ◽  
pp. 310-316 ◽  
Author(s):  
Garth R. Brown ◽  
Vindhya Amarasinghe ◽  
Gyula Kiss ◽  
John E. Carlson
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
White Spruce ◽  
Confocal Laser ◽  
Metaphase Chromosomes ◽  
Rdna Sites ◽  
Rdna Loci

We have localized the major ribosomal DNA (rDNA) loci on metaphase chromosomes and in interphase nuclei of white spruce (2n = 24) by fluorescence in situ hybridization. Hybridization sites of the biotin-labelled rDNA probe were detected using antibody–fluorochrome conjugates and a confocal laser scanning microscope. White spruce has at least 12, and possibly as many as 14, rDNA sites, 1 site present on each of seven separate chromosome pairs. This is one of the highest numbers of rDNA loci yet reported among plant species. The position of the rDNA loci together with secondary constriction patterns permit, for the first time, all homologous pairs of white spruce chromosomes to be distinguished. We discuss the application of molecular cytogenetics in studies relating to the organization and evolution of DNA sequences within conifer genomes.Key words: fluorescence in situ hybridization, Picea, rDNA, karyotype.

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