Timing of DNA replication of translocated Y chromosome sections in somatic cells of Drosophila melanogaster

Chromosoma ◽  
1969 ◽  
Vol 27 (4) ◽  
pp. 395-408 ◽  
Author(s):  
Carlotta Halfer ◽  
L. Tiepolo ◽  
C. Barigozzi ◽  
M. Fraccaro
Keyword(s):  
Drosophila Melanogaster ◽  
Dna Replication ◽  
Y Chromosome ◽  
Somatic Cells

scholarly journals MUTAGEN SPECIFICITY IN THE INDUCTION OF CHROMOSOMAL ABERRATIONS IN SOMATIC CELLS OF DROSOPHILA MELANOGASTER

Genetics ◽  
1977 ◽  
Vol 85 (2) ◽  
pp. 249-257
Author(s):  
S Pimpinelli ◽  
D Pignone ◽  
G Santini ◽  
M Gatti ◽  
G Olivieri
Keyword(s):  
Drosophila Melanogaster ◽  
Y Chromosome ◽  
Chromosomal Aberrations ◽  
Somatic Cells ◽  
X Rays ◽  
Aberration Frequency ◽  
Mutagen Specificity ◽  
Methane Sulphonate

ABSTRACT The distribution of chromosomal aberrations between and within chromosomes of male D. melanogaster somatic cells after treatment with UV has been analyzed.—Distribution of the breaks between chromosomes was largely nonrandom since we found a higher aberration frequency than that expected on the Y chromosome. Moreover, within the chromosomes the aberrations are clustered in the pericentromeric heterochromatic regions. The above distribution is compared with that of the breaks induced by X rays and methyl-methane-sulphonate (MMS) which were distributed in a different pattern.

scholarly journals Drosophila melanogaster male germ line-specific transcripts with autosomal and Y-linked genes.

Genetics ◽  
1993 ◽  
Vol 134 (1) ◽  
pp. 293-308 ◽  
Author(s):  
S R Russell ◽  
K Kaiser
Keyword(s):  
Drosophila Melanogaster ◽  
Y Chromosome ◽  
Germ Line ◽  
Stop Codon ◽  
Chromatin Condensation ◽  
Sperm Chromatin ◽  
Autosomal Gene ◽  
Cdna Clones ◽  
Autosomal Locus ◽  
Functional Protein

Abstract We have identified of set of related transcripts expressed in the germ line of male Drosophila melanogaster. Surprisingly, while one of the corresponding genes is autosomal the remainder are located on the Y chromosome. The autosomal locus, at 77F on chromosome arm 3L, corresponds to the previously described transcription unit 18c, located in the first intron of the gene for an RI subunit of cAMP-dependent protein kinase. The Y chromosome copies have been mapped to region h18-h19 on the cytogenetic map of the Y outside of any of the regions required for male fertility. In contrast to D. melanogaster, where Y-linked copies were found in nine different wild-type strains, no Y-linked copies were found in sibling species. Several apparently Y-derived cDNA clones and one Y-linked genomic clone have been sequenced. The Y-derived genomic DNA shares the same intron/exon structure as the autosomal copy as well as related flanking sequences suggesting that it transposed to the Y from the autosomal locus. However, this particular Y-linked copy cannot encode a functional polypeptide due to a stop codon at amino acid position 72. Divergence among five different cDNA clones ranges from 1.5 to 6% and includes a large number of third position substitutions. We have not yet obtained a full-length cDNA from a Y-linked gene and therefore cannot conclude that the D. melanogaster Y chromosome contains functional protein-coding genes. The autosomal gene encodes a predicted polypeptide with 45% similarity to histones of the H5 class and more limited similarity to cysteine-rich protamines. This protein may be a distant relative of the histone H1 family perhaps involved in sperm chromatin condensation.

Drosophila melanogaster H1 histone is phosphorylated stably

1987 ◽  
Vol 7 (11) ◽  
pp. 4118-4121
Author(s):  
D A Talmage ◽  
M Blumenfeld
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
Dna Replication ◽  
Cultured Cells ◽  
Phosphorylation Site ◽  
Developmentally Regulated ◽  
Central Domain ◽  
Salivary Gland Cells ◽  
Adult Head ◽  
The Relationship

Phosphorylation of histone H1 is developmentally regulated in Drosophila spp. It cannot be detected in preblastoderm embryos or polytene salivary gland cells, but in cellular blastoderm, postblastoderm embryo, and amitotic adult head nuclei, it occurs with a frequency of roughly 4 x 10(5) molecules per nucleus. We used pulse-labeling to study the relationship between H1 synthesis and modification in cultured cells. These results reveal that the H1-associated phosphate is stable and suggest that Drosophila H1 is synthesized, translocated to the nucleus, associated with chromatin, and then phosphorylated. Partial tryptic digestion of Drosophila H1 revealed that the phosphorylation site is located within the globular, central domain of the protein. Thus, the developmentally regulated phosphorylation of Drosophila H1 presents two contrasts with previously studied H1 phosphorylation. It is not correlated with DNA replication, and it is located in the central domain of the protein.

scholarly journals Recombinogenic activity of Pantoprazole® in somatic cells of Drosophila melanogaster

2015 ◽  
Vol 38 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Jeyson Césary Lopes ◽  
Nayane Moreira Machado ◽  
Rosiane Soares Saturnino ◽  
Júlio César Nepomuceno
Keyword(s):  
Drosophila Melanogaster ◽  
Somatic Cells

scholarly journals DNA replication of histone gene repeats in Drosophila melanogaster tissue culture cells: multiple initiation sites and replication pause sites.

1993 ◽  
Vol 13 (7) ◽  
pp. 4098-4106 ◽  
Author(s):  
T Shinomiya ◽  
S Ina
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Dna Replication ◽  
Present Report ◽  
Polymerase Chain Reaction Method ◽  
Mapping Method ◽  
Histone Gene ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Replication Intermediates

We showed previously that DNA replication initiates at multiple sites in the 5-kb histone gene repeating unit in early embryos of Drosophila melanogaster. The present report shows evidence that replication in the same chromosomal region initiates at multiple sites in tissue culture cells as well. First, we analyzed replication intermediates by the two-dimensional gel electrophoretic replicon mapping method and detected bubble-form replication intermediates for all fragments restricted at different sites in the repeating unit. Second, we analyzed bromodeoxyuridine-labeled nascent strands amplified by the polymerase chain reaction method and detected little differences in the size distribution of nascent strands specific to six short segments located at different sites in the repeating unit. These results strongly suggest that DNA replication initiates at multiple sites located within the repeating unit. We also found several replication pause sites located at 5' upstream regions of some histone genes.

scholarly journals Protective effects of proanthocyanidins of grape (Vitis vinifera L.) seeds on DNA damage induced by Doxorubicin in somatic cells of Drosophila melanogaster

2009 ◽  
Vol 47 (7) ◽  
pp. 1466-1472 ◽  
Author(s):  
Alexandre Azenha Alves de Rezende ◽  
Ulrich Graf ◽  
Zaira da Rosa Guterres ◽  
Warwick Estevam Kerr ◽  
Mário Antônio Spanó
Keyword(s):  
Dna Damage ◽  
Drosophila Melanogaster ◽  
Vitis Vinifera ◽  
Somatic Cells ◽  
Vitis Vinifera L ◽  
Protective Effects
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