Primary structure and expression pattern of the 33-kDa chitinase gene from the mycoparasitic fungus Trichoderma harzianum

1995 ◽  
Vol 28 (5) ◽  
pp. 478-483 ◽  
Author(s):  
M. Carmen Limón ◽  
José M. Lora ◽  
Irene García ◽  
Jesús de la Cruz ◽  
Antonio Llobell ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Trichoderma Harzianum ◽  
Primary Structure ◽  
Chitinase Gene ◽  
Mycoparasitic Fungus

Molecular characterization and heterologous expression of an endo-β-1,6-glucanase gene from the mycoparasitic fungus Trichoderma harzianum

1995 ◽  
Vol 247 (5) ◽  
pp. 639-645 ◽  
Author(s):  
José M. Lora ◽  
Jesús De la Cruz ◽  
Antonio Llobell ◽  
Tahía Benítez ◽  
José A. Pintor-Toro
Keyword(s):  
Heterologous Expression ◽  
Molecular Characterization ◽  
Trichoderma Harzianum ◽  
Mycoparasitic Fungus

scholarly journals Characterization of an Exo-β-d-Glucosaminidase Involved in a Novel Chitinolytic Pathway from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1

2003 ◽  
Vol 185 (17) ◽  
pp. 5175-5181 ◽  
Author(s):  
Takeshi Tanaka ◽  
Toshiaki Fukui ◽  
Haruyuki Atomi ◽  
Tadayuki Imanaka
Keyword(s):  
Escherichia Coli ◽  
Central Region ◽  
Primary Structure ◽  
Glycosyl Hydrolase ◽  
Chitinase Gene ◽  
Glycosyl Hydrolases ◽  
Galactosidase Activity ◽  
Hyperthermophilic Archaeon ◽  
Candidate Protein

ABSTRACT We previously clarified that the chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 produces diacetylchitobiose (GlcNAc2) as an end product from chitin. Here we sought to identify enzymes in T. kodakaraensis that were involved in the further degradation of GlcNAc2. Through a search of the T. kodakaraensis genome, one candidate gene identified as a putative β-glycosyl hydrolase was found in the near vicinity of the chitinase gene. The primary structure of the candidate protein was homologous to the β-galactosidases in family 35 of glycosyl hydrolases at the N-terminal region, whereas the central region was homologous to β-galactosidases in family 42. The purified protein from recombinant Escherichia coli clearly showed an exo-β-d-glucosaminidase (GlcNase) activity but not β-galactosidase activity. This GlcNase (GlmA Tk ), a homodimer of 90-kDa subunits, exhibited highest activity toward reduced chitobiose at pH 6.0 and 80°C and specifically cleaved the nonreducing terminal glycosidic bond of chitooligosaccharides. The GlcNase activity was also detected in T. kodakaraensis cells, and the expression of GlmA Tk was induced by GlcNAc2 and chitin, strongly suggesting that GlmA Tk is involved in chitin catabolism in T. kodakaraensis. These results suggest that T. kodakaraensis, unlike other organisms, possesses a novel chitinolytic pathway where GlcNAc2 from chitin is first deacetylated and successively hydrolyzed to glucosamine. This is the first report that reveals the primary structure of GlcNase not only from an archaeon but also from any organism.

Cloning and characterization of a chitinase (CHIT42) cDNA from the mycoparasitic fungus Trichoderma harzianum

1994 ◽  
Vol 27 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Irene García ◽  
José M. Lora ◽  
Jesús de la Cruz ◽  
Tahía Benítez ◽  
Antonio Llobell ◽  
...  
Keyword(s):  
Trichoderma Harzianum ◽  
Mycoparasitic Fungus

A putative catabolite-repressed cell wall protein from the mycoparasitic fungus Trichoderma harzianum

1994 ◽  
Vol 242 (4) ◽  
pp. 461-466 ◽  
Author(s):  
José M. Lora ◽  
Jésus de la Cruz ◽  
Tahía Benítez ◽  
Antonio Llobell ◽  
José A. Pintor-Toro
Keyword(s):  
Cell Wall ◽  
Trichoderma Harzianum ◽  
Cell Wall Protein ◽  
Mycoparasitic Fungus

scholarly journals Enzyme Diffusion from Trichoderma atroviride (=T. harzianum P1) to Rhizoctonia solani Is a Prerequisite for Triggering of Trichoderma ech42 Gene Expression before Mycoparasitic Contact

2000 ◽  
Vol 66 (5) ◽  
pp. 2232-2234 ◽  
Author(s):  
Cornelia Kullnig ◽  
Robert L. Mach ◽  
Matteo Lorito ◽  
Christian P. Kubicek
Keyword(s):  
Gene Expression ◽  
Rhizoctonia Solani ◽  
Trichoderma Harzianum ◽  
Chitinase Gene ◽  
Trichoderma Atroviride ◽  
Dialysis Membranes

ABSTRACT A plate confrontation experiment is commonly used to study the mechanism by which Trichoderma spp. antagonize and parasitize other fungi. Previous work with chitinase gene expression (ech42) during the precontact period of this process in which cellophane and dialysis membranes separated Trichoderma harzianum and its host Rhizoctonia solani resulted in essentially opposite results. Here, we show that cellophane membranes are permeable to proteins up to at least 90 kDa in size but that dialysis membranes are not. ech42 was expressed during the precontact stage of the confrontation between Trichoderma atroviride and its host only if the cellophane was placed between the two fungi. These results are consistent with enzyme diffusion from T. atroviride to R. solani generating the trigger of ech42 gene expression.

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