Cloning and characterization of a chitinase (CHIT42) cDNA from the mycoparasitic fungus Trichoderma harzianum

1994 ◽  
Vol 27 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Irene García ◽  
José M. Lora ◽  
Jesús de la Cruz ◽  
Tahía Benítez ◽  
Antonio Llobell ◽  
...  
Keyword(s):  
Trichoderma Harzianum ◽  
Mycoparasitic Fungus

Purification and characterization of a β-mannanase from Trichoderma harzianum strain T4

2004 ◽  
Vol 57 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Humberto Malheiros Ferreira ◽  
Edivaldo Ximenes Ferreira Filho
Keyword(s):  
Trichoderma Harzianum ◽  
Purification And Characterization

scholarly journals ISOLATION AND CHARACTERIZATION OF CARBENDAZIM DEGRADING Trichoderma harzianum RIFAI MUTANTS

2010 ◽  
Vol 20 ◽  
Author(s):  
ITAMAR SOARES DE MELO ◽  
CÉLIA MARIA MAGANHOTTO DE S. SILVA ◽  
JANE L. FAULL
Keyword(s):  
Trichoderma Harzianum ◽  
Uv Light ◽  
Light Exposure ◽  
Wild Type ◽  
Pesticide Degradation ◽  
Isolation And Characterization ◽  
Degradation Ability

Mutants of Trichoderma harzianum Rifai, obtained after ultraviolet (UV) light exposure, showed high resistant to the fungicide benomyl. A mutant (2B6) was capable of degrading carbendazim, other fungicide of the benzimidazole fungicide. This mutant degraded 41.5% of the molecule within five days. This and others mutants (2B1 and 2B2) presented variation in size and frequency of uni-nucleated and/or bi-nucleated spores compared to the wild type. Four primers generated RAPDs patterns that allowed the mutant to be differentiated from the wild-type. It is concluded that using UV mutagenization, it is feasible to obtain strains of T. harzianum with improved pesticide degradation ability.

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