The periodic acid-schiff reaction in neutrophil leukocytes-influence of fixation and amylase digestion

1966 ◽  
Vol 6 (2) ◽  
pp. 112-119 ◽  
Author(s):  
G�sta Gahrton
Keyword(s):  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Schiff Reaction ◽  
Neutrophil Leukocytes

scholarly journals Determination of the glycogen content in single neutrophil leukocytes using a micromodel of leukocyte glycogen.

1975 ◽  
Vol 23 (1) ◽  
pp. 59-64 ◽  
Author(s):  
G Gahrton ◽  
I Olsson ◽  
A Dahlqvist
Keyword(s):  
Periodic Acid ◽  
Liver Glycogen ◽  
Mean Value ◽  
Acid Oxidation ◽  
Periodic Acid Schiff ◽  
Periodic Acid Oxidation ◽  
Normal Human ◽  
Schiff Reaction ◽  
Neutrophil Leukocytes

The kinetics of the periodic acid oxidation as part of the periodic acid-Schiff reaction was studied by combined microinterferometry and microspectrophotometry in micromodel systems of liver glycogen and leukocyte glycogen as well as in neutrophil leukocytes. The initial formation of Schiff-positive chromogens was more rapid in neutrophil leukocytes than in liver or leukocyte glycogen. The chromogen formation was, however, practically complete within 60 min in both neutrophil leukocytes and leukocyte glycogen, but this did not appear to be the case in liver glycogen. Differences in the rate of chromogen formation may depend on various factors such as differences in the source and treatment of the glycogen. The complete periodic acid-Schiff reaction appears to be a measure of the glycogen amount in neutrophil leukocytes and the microdroplet system of leukocyte glycogen is considered to be an appropriate model for the estimation of the glycogen amount in single neutrophil leukocytes. A mean value of 13.3 10-12 g glycogen per normal human neutrophil was found.

scholarly journals NORMAL HUMAN NEUTROPHIL LEUKOCYTES AS A REFERENCE SYSTEM FOR THE MICROSPECTROPHOTOMETRICALLY QUANTITATED PERIODIC ACID-SCHIFF REACTION

1966 ◽  
Vol 14 (1) ◽  
pp. 45-48 ◽  
Author(s):  
GÖSTA GAHRTON
Keyword(s):  
Human Neutrophil ◽  
Reference System ◽  
Periodic Acid ◽  
Reference Series ◽  
Periodic Acid Schiff ◽  
Reactive Material ◽  
Pas Reaction ◽  
Normal Human ◽  
Schiff Reaction ◽  
Neutrophil Leukocytes

Normal human neutrophil leukocytes are suggested as references for a microspectrophotometrically quantitated PAS reaction in biological material. Analysis of variance of values for the total extinction at 546 mµ in PAS-stained, single, normal neutrophils proved these cells to be suitable references. Methanol-fixed, smeared neutrophils could be stored for several months without changing their amount of PAS reactive material. A person with a mean for the amount of PAS reactive material in neutrophils that lay close to the average in a normal material of 20 persons was chosen as the original reference subject. New reference series of neutrophils were related to the original one, thus obtaining a continuous reference system. The results are based upon microspectrophotometric measurements of about 3500 individual neutrophils.

scholarly journals The Periodic Acid-Schiff Reaction in Neutrophil Leukocytes in Untreated and Myleran-Treated Chronic Myelocytic Leukemia

Blood ◽  
1966 ◽  
Vol 28 (4) ◽  
pp. 544-552 ◽  
Author(s):  
GÖSTA GAHRTON
Keyword(s):  
Periodic Acid ◽  
Quantitative Measure ◽  
Normal Subjects ◽  
Chronic Myelocytic Leukemia ◽  
Periodic Acid Schiff ◽  
Reactive Material ◽  
Myelocytic Leukemia ◽  
Pas Reaction ◽  
Schiff Reaction ◽  
Neutrophil Leukocytes

Abstract Microspectrophotometric quantification of the periodic acid-Schiff (PAS) reaction has been performed in about 3,000 individual mature neutrophil leukocytes from 20 normal subjects and 23 patients with chronic myelocytic leukemia (CML), of whom 10 were untreated or in relapse and 13 in partial remission or remission. The PAS reaction was taken as a quantitative measure of the cellular amount of PAS reactive material (PASMa), most probably equivalent to glycogen. The mean amount of PASMa in neutrophils from untreated and relapse cases of CML was 43 per cent less than in normal neutrophils. The frequency distribution of PASMa per cell was normal in individual cases. In clinical remission after Myleran treatment, the neutrophils contained normal amounts of PASMa.

scholarly journals Effect of activity on performance and morphology in ischaemic rat slow muscles

1990 ◽  
Vol 152 (1) ◽  
pp. 265-279
Author(s):  
A. Corsi ◽  
A. L. Granata ◽  
O. Hudlicka
Keyword(s):  
Blood Supply ◽  
Periodic Acid ◽  
Capillary Density ◽  
Muscle Performance ◽  
Similar Proportion ◽  
Periodic Acid Schiff ◽  
Rat Soleus Muscle ◽  
Improved Performance ◽  
Isometric Twitch ◽  
Schiff Reaction

Muscle performance and structure was studied in rat soleus muscle with limited blood supply in combination with chronic muscle stimulation. Blood supply to the lower leg was restricted by ligation of the common iliac artery, electrodes were implanted in the vicinity of the sciatic nerve and ankle flexors were denervated. Three days later, soleus and gastrocnemius muscles were stimulated at 4 Hz four times a day for a period of 20 min with 2 h intervals between stimulations; this procedure was continued for 4 days. Muscle performance, histochemistry and ultrastructure were studied on the eighth day after operation in these muscles and in ischaemic unstimulated muscles with denervated ankle flexors. Both were compared with control animals. Muscles with limited blood supply developed less isometric twitch tension than control muscles (peak twitch tension in ischaemic muscle was 60.3 +/− 4.8 g g-1 muscle, mean +/− S.E.M., compared to 79.7 +/− 6.9 g g-1 in control muscle; tensions after 5 min contraction were 54.5 +/− 5.5 g g-1 in ischaemic muscle compared to 70.6 +/− 6 g g-1 in controls). Stimulated muscles with limited blood supply had higher peak (85 +/− 16.6 g g-1) and final (87 +/− 12 g g-1) tensions, and also fatigued less than muscles with limited blood supply but no stimulation. Histochemical estimation of capillary density (by staining for alkaline phosphatase) and slow (SO) and fast (FOG) fibres (by myosin ATPase staining) revealed similar capillary to fibre ratios (2.5) and a similar proportion of FOG fibres (around 18%) in all muscles. The proportion of glycogen-depleted fibres (estimated from the periodic acid Schiff reaction, PAS) in muscles removed from animals 10 min after a 5 min period of isometric twitches was significantly lower in ischaemic muscles (45.1 +/− 1.9%) than in control (80.5 +/− 1.5%) or chronically stimulated ischaemic muscles (67.3 +/− 4.0%). Electron microscopy showed disorganised myofibrils with Z-line streaming in 7.48 +/− 3.04% of fibres in muscles with limited blood supply. Swollen and degenerated mitochondria, dilated sarcoplasmic reticulum and areas of disrupted sarcolemma were also observed. Stimulated ligated muscles showed a significantly lower proportion of fibres with disorganised filaments (0.65 +/− 0.32%) and other signs of damage were much less frequent. The reduced damage and improved performance of chronically stimulated slow muscle may be the result of improved microcirculation, preventing accumulation of lactate.

scholarly journals Activation and activity of glycosylated KLKs 3, 4 and 11

2018 ◽  
Vol 399 (9) ◽  
pp. 1009-1022 ◽  
Author(s):  
Shihui Guo ◽  
Peter Briza ◽  
Viktor Magdolen ◽  
Hans Brandstetter ◽  
Peter Goettig
Keyword(s):  
Physiological Function ◽  
Periodic Acid ◽  
Hek293 Cells ◽  
Secretory Expression ◽  
Detailed Characterization ◽  
Periodic Acid Schiff ◽  
Clinical Biomarkers ◽  
Schiff Reaction

Abstract Human kallikrein-related peptidases 3, 4, 11, and KLK2, the activator of KLK3/PSA, belong to the prostatic group of the KLKs, whose major physiological function is semen liquefaction during the fertilization process. Notably, these KLKs are upregulated in prostate cancer and are used as clinical biomarkers or have been proposed as therapeutic targets. However, this potential awaits a detailed characterization of these proteases. In order to study glycosylated prostatic KLKs resembling the natural proteases, we used Leishmania (LEXSY) and HEK293 cells for secretory expression. Both systems allowed the subsequent purification of soluble pro-KLK zymogens with correct propeptides and of the mature forms. Periodic acid-Schiff reaction, enzymatic deglycosylation assays, and mass spectrometry confirmed the glycosylation of these KLKs. Activation of glycosylated pro-KLKs 4 and 11 turned out to be most efficient by glycosylated KLK2 and KLK4, respectively. By comparing the glycosylated prostatic KLKs with their non-glycosylated counterparts from Escherichia coli, it was observed that the N-glycans stabilize the KLK proteases and change their activation profiles and their enzymatic activity to some extent. The functional role of glycosylation in prostate-specific KLKs could pave the way to a deeper understanding of their biology and to medical applications.

scholarly journals THE HISTOCHEMICAL INTERPRETATION OF THE COMPLEX RESULTS OF METHYLATION UPON GASTROINTESTINAL TRACT MUCINS, WITH SPECIAL REFERENCE TO THE PERIODIC ACID-SCHIFF REACTIVITY

1974 ◽  
Vol 22 (10) ◽  
pp. 986-991 ◽  
Author(s):  
P. E. REID ◽  
C. F. A. CULLING ◽  
W. L. DUNN
Keyword(s):  
Sialic Acid ◽  
Gastrointestinal Tract ◽  
Special Reference ◽  
Periodic Acid ◽  
Separate Study ◽  
Sulfate Ester ◽  
Periodic Acid Schiff ◽  
Smith Degradation ◽  
Vicinal Diol ◽  
Schiff Reaction

The histochemical use of methylation has complex results; particularly in respect of the periodic acid-Schiff reaction, these are analyzed and discussed. Methods are described which allow the separate study of the following effects: (a) the removal of the KOH/periodic acid-Schiff effect; (b) removal of sialic acid from a potential vicinal diol; and (c) the removal of O-sulfate ester from a potential vicinal diol. The use of the Smith degradation technique, in addition to the above, also allows inferences to be drawn in respect of the structure of the mucins (glycoproteins) being investigated.

scholarly journals Acute lymphoblastic leukemia with unusual cytoplasmic granulation: a morphologic, cytochemical, and ultrastructural study

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 406-411 ◽  
Author(s):  
J Fradera ◽  
E Velez-Garcia ◽  
JG White
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Acute Leukemias ◽  
Ultrastructural Studies ◽  
Sudan Black B ◽  
Blast Cells ◽  
Chediak Higashi Syndrome ◽  
Schiff Reaction

Abstract The classification of the acute leukemias depends mainly on the morphologic and cytochemical evaluation of the blast forms. One of the main accepted morphologic criteria in the differentiation between acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML) is the absence of granules in the blast cells of ALL. We evaluated a patient with ALL in whom granules were present in the cytoplasm of 35% of the blast cells, as seen in AML. Cytochemical evaluation was performed, including periodic acid-Schiff reaction, Sudan black B, alpha-naphthyl acetate, alpha-naphthyl butyrate, naphthol AS-D chloroacetate, and acid phosphatase stains. The results of these studies confirmed the morphologic impression and diagnosis of ALL. Ultrastructural evaluation revealed that the granules consisted of many tiny vesicles closely packed together in a proteinaceous matrix, resembling to some extent the inclusions described in lymphocytes in the Chediak-Higashi syndrome, but clearly different. The morphologic, cytochemical, and ultrastructural studies of this unique case are presented in detail. To our knowledge, this is the first time that such granules have been described in blast cells of ALL.

The periodic acid-Schiff reaction in the cornea of the developing chick

1960 ◽  
Vol 121 (5) ◽  
pp. 351-368 ◽  
Author(s):  
Ronan O'Rahilly ◽  
David B. Meyer
Keyword(s):  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Schiff Reaction

scholarly journals A VERTICAL MICROSYSTEM FOR DISCONTINUOUS ELECTROPHORESIS OF INSECT TISSUE PROTEINS USING THIN SHEETS OF POLYACRYLAMIDE GEL

1972 ◽  
Vol 20 (5) ◽  
pp. 368-384 ◽  
Author(s):  
ANITA C. BEEN ◽  
ELLEN M. RASCH
Keyword(s):  
Periodic Acid ◽  
Polyacrylamide Gel ◽  
Thin Sheets ◽  
Periodic Acid Schiff ◽  
Sudan Black B ◽  
Tissue Extracts ◽  
Salivary Gland Cells ◽  
Schiff Reaction ◽  
Coomassie Brilliant ◽  
Nonspecific Esterases

Proteins extracted from individual pairs of salivary glands or other larval tissues of Sciara coprophila (Diptera) were separated in a vertical microsystem for discontinuous electrophoresis using thin sheets of polyacrylamide gel cast in multiple layers of varying pore size. After electrophoresis at 150 volts for 40 min, gels were stained ( a) for total proteins with Coomassie brilliant blue, ( b) for glycoproteins with the periodic acid-Schiff reaction, ( c) for lipoproteins with Sudan black B or ( d) for nonspecific esterases with fast blue RR as coupler and α-naphthol acetate as substrate. Sequential application of these reactions to individual gel sectors permitted direct comparisons of protein profiles for 15-20 different samples of tissue extracts carried on a single gel sheet in adjacent lanes and thus subjected to identical conditions of electrophoresis. Representative photographs and densitometric scans are presented to show the suitability of thin gel sheets for autoradiography and for both qualitative and quantitative evaluation of tissue-specific differences in patterns of protein banding found for salivary gland cells, the gastric ceca, or the hemolymph of individual Sciara larvae sacrificed at particular stages of fourth instar development. Innovative details of methodology are presented, including the use of a microspectrophotometer to scan electropherograms of insect proteins and several types of human blood serum.

Sign in / Sign up

Export Citation Format

Share Document