A new method for the histochemical demonstration of steroid producing cells in human tissues

1965 ◽  
Vol 5 (3) ◽  
pp. 234-243 ◽  
Author(s):  
M. J. Hardonk
Keyword(s):  
Histochemical Demonstration ◽  
New Method ◽  
Human Tissues

A new method for the histochemical demonstration ofO-acyl sugars in human colonic epithelial glycoproteins

1988 ◽  
Vol 20 (9) ◽  
pp. 510-518 ◽  
Author(s):  
P. E. Reid ◽  
D. Volz ◽  
K. Y. Cho ◽  
D. A. Owen
Keyword(s):  
Histochemical Demonstration ◽  
New Method ◽  
Acyl Sugars

scholarly journals A novel tetrazolium method for peroxidase histochemistry and immunohistochemistry.

1991 ◽  
Vol 39 (4) ◽  
pp. 541-544 ◽  
Author(s):  
G I Murray ◽  
C O Foster ◽  
S W Ewen
Keyword(s):  
Peroxidase Activity ◽  
Histochemical Demonstration ◽  
New Method ◽  
Tetrazolium Salt ◽  
Immunoperoxidase Technique ◽  
Red Cell ◽  
Final Reaction Product ◽  
Biopsy Specimens ◽  
Oxidation Of Phenol ◽  
Indirect Immunoperoxidase

We developed a new method for the histochemical demonstration of peroxidase. This method, which has a novel reaction mechanism, is based on the oxidation of phenol by peroxidase and coupling of this reaction to the reduction of a tetrazolium salt, with the deposition of an insoluble formazan at sites of enzyme activity. This new method was compared with an established diaminobenzidine (DAB) technique for peroxidase histochemistry and immunohistochemistry. Although both methods identified peroxidase activity in myeloid cells of bone marrow biopsy specimens, there was no interference from red cell pseudoperoxidase activity with the phenol-tetrazolium method, in contrast to the diaminobenzidine method. The detection of cytokeratin using an indirect immunoperoxidase technique was compared with both methods for demonstrating peroxidase activity. The phenol-tetrazolium method gave results similar to that obtained with DAB and appeared to be at least as sensitive as DAB in detecting low amounts of antigen. In addition, the production of a formazan as the final reaction product means that the phenol-tetrazolium method is ideally suited for quantitative peroxidase histochemistry. Therefore, the phenol-tetrazolium method represents a useful alternative method to DAB and for certain applications offers significant advantages over DAB.

scholarly journals Histochemical demonstration of unsaturated hydrophilic lipids with palladium chloride.

1977 ◽  
Vol 25 (3) ◽  
pp. 200-205 ◽  
Author(s):  
J A Kiernan
Keyword(s):  
Solvent Extraction ◽  
Standard Technique ◽  
Histochemical Demonstration ◽  
New Method ◽  
Histochemical Method ◽  
Palladium Chloride ◽  
Unsaturated Lipids ◽  
Metallic Palladium

Compounds in which olefinic linkages are accessible to aqueous reagents reduce the chloropalladite ion [PdCl4]2-, to metallic palladium. This reaction is used in a histochemical method whereby hydrophilic unsaturated lipids are stained dark brown or black. The specificity of the new method has been confirmed by means of solvent-extraction and chemical blocking procedures and by comparison with other histochemical techniques. Yellow staining of collagen, keratin and cytoplasm is probably due to attachment of the chloropalladite anion to proteins. The yellow background can be largely decolorized by treating the sections with aqueous pyridine, which forms colorless complexes with divalent palladium. A standard technique for staining with palladium is presented and the method is discussed in relation to other histochemical procedures that demonstrate unsaturated lipids.

scholarly journals Improvement in the Histochemical Localization of Leucine Aminopeptidase with a New Substrate, L-Leucyl-4-Methoxy-2-Naphthylamide

1960 ◽  
Vol 7 (2) ◽  
pp. 261-264 ◽  
Author(s):  
Marvin M. Nachlas ◽  
Benito Monis ◽  
David Rosenblatt ◽  
Arnold M. Seligman
Keyword(s):  
Leucine Aminopeptidase ◽  
Hydrolysis Product ◽  
Histochemical Demonstration ◽  
New Method ◽  
Lipid Solubility ◽  
Rapid Rate ◽  
Frozen Sections ◽  
Enzyme Localization ◽  
Copper Chelate ◽  
Fresh Frozen

A new method for the histochemical demonstration of leucine aminopeptidase in fresh frozen sections was developed with the substrate L-leucyl-4-methoxy-2-naphthylamide. The superior enzyme localization is due to the more rapid rate of coupling of the hydrolysis product, 4-methoxy-2-naphthylamine as compared to 2-naphthylamine itself, and to the low lipid solubility and high substantivity for protein of the copper chelate of the dye formed on coupling with tetrazotized diorthoanisidine. A comparison of the old and the new method is illustrated, and a description is given of the localization of leucine aminopeptidase in the tissues of the rat and man.

scholarly journals A HISTOCHEMICAL STUDY OF SUBSTRATE SPECIFICITY FOR THE STEROID 3β-ol DEHYDROGENASE AND ISOMERASE SYSTEMS IN HUMAN OVARY AND TESTIS

1964 ◽  
Vol 12 (12) ◽  
pp. 880-889 ◽  
Author(s):  
B. GOLDBERG ◽  
G. E. S. JONES ◽  
H. I. BORKOWF
Keyword(s):  
Substrate Specificity ◽  
Corpus Luteum ◽  
Histochemical Study ◽  
Enzyme System ◽  
Histochemical Demonstration ◽  
Human Testis ◽  
Human Tissues ◽  
Human Ovary ◽  
Homeostatic Mechanisms ◽  
Endocrine Tissues

Either the enzyme which oxidizes 3β-ol groups in C-19 and C-21 steroids is properly designated a steroid-3β-hydroxy dehydrogenase or there is a multiplicity of such dehydrogenases. Although DHA is a satisfactory substrate for the human ovary, adrenal, and placenta, it is not generally so for the human testis. Pregnenolone is only at times a moderately satisfactory substrate in the ovary and is not satisfactory for the testis. In the present histochemical study two Δ4,3β-ol steroids have been reported as satisfactory substrates for human endocrine tissues as well as liver and intestine. These new substrates make it possible to demonstrate activity in human testicular tissues and at probable sites of androgen production. If these tenets hold, they indicate that it is more satisfactory to use unphysiologic substrates for the histochemical demonstration of enzymes as this will obviate the interplay of homeostatic mechanisms which must constantly be at work to preserve the cellular equilibrium. Although the data might suggest that the human testis, in contrast to the ovary, is deficient in isomerizing capacity and that this step precedes oxidation of the 3β-ol group, the biochemical data do not support this theory. A more probable explanation is that either DHA, its reaction product 5-androstenedione, or both, inhibit the human testicular enzyme system, while progesterone inhibits that of the corpus luteum; thus a difference between the dehydrogenases of these two human tissues appears to exist.

Microstructural comparison of synthetic bioceramics with natural bioceramics

1991 ◽  
Vol 49 ◽  
pp. 38-39
Author(s):  
Shulin Wen ◽  
Jingwei Feng ◽  
A. Krajewski ◽  
A. Ravaglioli
Keyword(s):  
Recent Work ◽  
Human Body ◽  
Human Tissues ◽  
Main Constituent ◽  
Laboratory Furnace ◽  
Chinese Adult ◽  
Human Enamel ◽  
Biological Compatibility ◽  
Synthetic Hydroxyapatite ◽  
Synthetic Work

Hydroxyapatite bioceramics has attracted many material scientists as it is the main constituent of the bone and the teeth in human body. The synthesis of the bioceramics has been performed for years. Nowadays, the synthetic work is not only focused on the hydroapatite but also on the fluorapatite and chlorapatite bioceramics since later materials have also biological compatibility with human tissues; and they may also be very promising for clinic purpose. However, in comparison of the synthetic bioceramics with natural one on microstructure, a great differences were observed according to our previous results. We have investigated these differences further in this work since they are very important to appraise the synthetic bioceramics for their clinic application.The synthetic hydroxyapatite and chlorapatite were prepared according to A. Krajewski and A. Ravaglioli and their recent work. The briquettes from different hydroxyapatite or chlorapatite powders were fired in a laboratory furnace at the temperature of 900-1300°C. The samples of human enamel selected for the comparison with synthetic bioceramics were from Chinese adult teeth.

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