The effects of chronic serum sickness on albumin distribution and glucose utilization in rat brain

1991 ◽  
Vol 81 (3) ◽  
pp. 312-317
Author(s):  
H. Nakata ◽  
A. Shimizu ◽  
A. Tajima ◽  
S. Z. Lin ◽  
K. Gruber ◽  
...  
Keyword(s):  
Rat Brain ◽  
Glucose Utilization ◽  
Serum Sickness ◽  
Albumin Distribution

Quantitative Measurements of Regional Glucose Utilization and Rate of Valine Incorporation into Proteins by Double-Tracer Autoradiography in the Rat Brain Tumor Model

1989 ◽  
Vol 9 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Michihiro Kirikae ◽  
Mirko Diksic ◽  
Y. Lucas Yamamoto
Keyword(s):  
Protein Synthesis ◽  
Brain Tumor ◽  
Rat Brain ◽  
Brain Tissue ◽  
Glucose Utilization ◽  
Tumor Model ◽  
Normal Brain ◽  
Normal Brain Tissue ◽  
Rat Brain Tumor ◽  
Brain Tumor Model

We examined the rate of glucose utilization and the rate of valine incorporation into proteins using 2-[18F]fluoro-2-deoxyglucose and L-[1-14C]-valine in a rat brain tumor model by quantitative double-tracer autoradiography. We found that in the implanted tumor the rate of valine incorporation into proteins was about 22 times and the rate of glucose utilization was about 1.5 times that in the contralateral cortex. (In the ipsilateral cortex, the tumor had a profound effect on glucose utilization but no effect on the rate of valine incorporation into proteins.) Our findings suggest that it is more useful to measure protein synthesis than glucose utilization to assess the effectiveness of antitumor agents and their toxicity to normal brain tissue. We compared two methods to estimate the rate of valine incorporation: “kinetic” (quantitation done using an operational equation and the average brain rate coefficients) and “washed slices” (unbound labeled valine removed by washing brain slices in 10% thrichloroacetic acid). The results were the same using either method. It would seem that the kinetic method can thus be used for quantitative measurement of protein synthesis in brain tumors and normal brain tissue using [11C]-valine with positron emission tomography.

scholarly journals Effect of KB-2796 upon local cerebral glucose utilization in the rat brain

1987 ◽  
Vol 43 ◽  
pp. 98
Author(s):  
Akira Yamashita ◽  
Akemi Hayashi ◽  
Akio Ozaki ◽  
Takayuki Sukamoto ◽  
Keizo Ito
Keyword(s):  
Rat Brain ◽  
Glucose Utilization ◽  
Local Cerebral Glucose Utilization ◽  
Cerebral Glucose Utilization

The effects of intravenous norepinephrine on the local coupling between glucose utilization and blood flow in the rat brain

1983 ◽  
Vol 398 (2) ◽  
pp. 134-138 ◽  
Author(s):  
W. Kuschinsky ◽  
S. Suda ◽  
R. B�nger ◽  
S. Yaffe ◽  
L. Sokoloff
Keyword(s):  
Blood Flow ◽  
Rat Brain ◽  
Glucose Utilization ◽  
Local Coupling

DNA synthesis, blood flow, and glucose utilization in experimental rat brain tumors

1989 ◽  
Vol 70 (1) ◽  
pp. 86-91 ◽  
Author(s):  
Akira Watanabe ◽  
Ryuichi Tanaka ◽  
Norio Takeda ◽  
Kazuo Washiyama
Keyword(s):  
Blood Flow ◽  
Brain Tumors ◽  
Rat Brain ◽  
Glucose Utilization ◽  
Acid Synthesis ◽  
S Phase ◽  
Immunoperoxidase Staining ◽  
Content Type ◽  
Contralateral Cortex ◽  
Autoradiographic Technique

✓ The relationships between distribution of deoxyribonucleic acid (DNA)-synthesizing cells (S-phase cells) and blood flow and glucose utilization were investigated in rat brain tumors using an autoradiographic technique and immunoperoxidase staining for bromodeoxyuridine (BUdR). Two strains of rat brain tumor were used: strain A and B, both induced by the Rous sarcoma virus. Strain A was biologically more malignant than strain B. The blood flow was unevenly distributed in the tumor; compared with the contralateral cortex, the average blood flow in the tumor was about 50% in strain A and 60% in strain B. The distribution of blood flow did not correlate with the distribution of S-phase cells or with the distribution of vessels in the tumor in either strain A or B. The average glucose utilization in strain A was about 250% and in strain B about 170% of that of the contralateral cortex. The high glucose utilization area correlated well with the distribution of BUdR-positive nuclei in strain B. These findings suggest that the biological malignancy of a tumor correlates with glucose utilization rather than with blood flow, and that malignant brain tumors show a marked increase in glucose utilization for nucleic acid synthesis.

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