Dichlorovinyl cysteine (DCVC) in the mouse kidney: tissue-binding and toxicity after glutathione depletion and probenecid treatment

1989 ◽  
Vol 63 (5) ◽  
pp. 345-350 ◽  
Author(s):  
Per Ola Darnerud ◽  
Ingvar Brandt ◽  
Vernon J. Feil ◽  
Jerome E. Bakke
Keyword(s):  
Kidney Tissue ◽  
Mouse Kidney ◽  
Glutathione Depletion ◽  
Tissue Binding ◽  
Dichlorovinyl Cysteine

YAP1 Overexpression Is Associated with Kidney Dysfunction in Lupus Nephritis

Pathobiology ◽  
2021 ◽  
pp. 1-12
Author(s):  
Ying Xie ◽  
Yuanyuan Ruan ◽  
Huimei Zou ◽  
Yixin Wang ◽  
Xin Wu ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Epithelial Mesenchymal Transition ◽  
Kidney Tissue ◽  
Mouse Kidney ◽  
Experimental Comparison ◽  
Control Group ◽  
Mrna Levels ◽  
Mesenchymal Transition ◽  
Protein Levels

<b><i>Objective:</i></b> The goal of the present study was to determine the expression of yes-associated protein 1 (YAP1) in renal tissues of mice with lupus nephritis (LN) and elucidate its role in the progression of renal fibrosis. <b><i>Methods:</i></b> C57BL/6 mice and MRL/lpr mice were selected for experimental comparison. Mouse kidney tissues were removed and sectioned for hematoxylin and eosin staining, Masson’s trichome staining, Sirius staining, and immunohistochemistry. The mRNA and protein levels of YAP1 in mouse kidney tissues were detected, and the correlation between YAP1 and fibronectin (FN) mRNA levels was analyzed. Mouse renal epithelial cells were used for in vitro experiments. After transfection and stimulation, the cells were divided into 4 groups, namely the C57BL/6 serum group (group 1), the MRL/lpr serum group (group 2), the MRL/lpr serum + siRNA-negative control group (group 3), and the MRL/lpr serum + siRNA-YAP1 group (group 4). Epithelial-mesenchymal transition (EMT) markers in each group were detected by Western blotting and immunofluorescence staining. Serum creatinine, blood urea nitrogen, and urinary protein levels were detected and assessed for their correlation with YAP1 mRNA levels by Spearman’s analysis. <b><i>Results:</i></b> Compared to C57BL/6 mice, MRL/lpr mice exhibited obvious changes in fibrosis in renal tissues. In addition, YAP1 expression was significantly higher in the renal tissues of MRL/lpr mice than in those of C57BL/6 mice, and YAP1 mRNA levels were positively correlated with those of FN. YAP1 silencing in lupus serum-stimulated cells could effectively relieve serum-induced EMT. Finally, we observed that YAP1 mRNA levels in mouse kidney tissue were significantly and positively correlated with the degree of renal function injury. <b><i>Conclusion:</i></b> YAP1 expression in the kidney tissues of LN mice was higher than that observed in normal mice, indicating that YAP1 may play an important role in the occurrence and development of LN.

scholarly journals Time- and dose rate-related effects of internal 177Lu exposure on gene expression in mouse kidney tissue

2014 ◽  
Vol 41 (10) ◽  
pp. 825-832 ◽  
Author(s):  
Emil Schüler ◽  
Nils Rudqvist ◽  
Toshima Z. Parris ◽  
Britta Langen ◽  
Johan Spetz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dose Rate ◽  
Kidney Tissue ◽  
Mouse Kidney

scholarly journals High throughput single cell RNA-seq of developing mouse kidney and human kidney organoids reveals a roadmap for recreating the kidney

2017 ◽  
Author(s):  
Alexander N. Combes ◽  
Belinda Phipson ◽  
Luke Zappia ◽  
Kynan T. Lawlor ◽  
Pei Xuan Er ◽  
...  
Keyword(s):  
Single Cell ◽  
Kidney Tissue ◽  
Human Kidney ◽  
Population Based ◽  
Mouse Kidney ◽  
Rna Seq ◽  
Kidney Morphogenesis ◽  
Human Ipsc ◽  
Kidney Organoids

AbstractRecent advances in our capacity to differentiate human pluripotent stem cells to human kidney tissue are moving the field closer to novel approaches for renal replacement. Such protocols have relied upon our current understanding of the molecular basis of mammalian kidney morphogenesis. To date this has depended upon population based-profiling of non-homogenous cellular compartments. In order to improve our resolution of individual cell transcriptional profiles during kidney morphogenesis, we have performed 10x Chromium single cell RNA-seq on over 6000 cells from the E18.5 developing mouse kidney, as well as more than 7000 cells from human iPSC-derived kidney organoids. We identified 16 clusters of cells representing all major cell lineages in the E18.5 mouse kidney. The differentially expressed genes from individual murine clusters were then used to guide the classification of 16 cell clusters within human kidney organoids, revealing the presence of distinguishable stromal, endothelial, nephron, podocyte and nephron progenitor populations. Despite the congruence between developing mouse and human organoid, our analysis suggested limited nephron maturation and the presence of ‘off target’ populations in human kidney organoids, including unidentified stromal populations and evidence of neural clusters. This may reflect unique human kidney populations, mixed cultures or aberrant differentiation in vitro. Analysis of clusters within the mouse data revealed novel insights into progenitor maintenance and cellular maturation in the major renal lineages and will serve as a roadmap to refine directed differentiation approaches in human iPSC-derived kidney organoids.

scholarly journals Deep Penetration Microscopic Imaging with Non-Diffracting Airy Beams

Membranes ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 391
Author(s):  
Yong Guo ◽  
Yangrui Huang ◽  
Jin Li ◽  
Luwei Wang ◽  
Zhigang Yang ◽  
...  
Keyword(s):  
3D Structure ◽  
Kidney Tissue ◽  
Mouse Kidney ◽  
Deep Penetration ◽  
Imaging Method ◽  
Layer By Layer ◽  
Microscopic Imaging ◽  
Volumetric Imaging ◽  
Airy Beam ◽  
Volume Imaging

We report a deep penetration microscopic imaging method with a non-diffracting Airy beam. The direct mapping of volume imaging in free space shows that the axial imaging range of the Airy beam is approximately 4 times that of the traditional Gaussian beam along the axial direction while maintaining a narrow lateral width. Benefiting from its non-diffracting property, the microscopic imaging with Airy beam illumination can acquire image structures through turbid medium and capture a volumetric image in a single frame. We demonstrate the penetration ability of the Airy microscopic imaging through a strongly scattering environment with 633 nm and 780 nm lasers. The performances of the volumetric imaging method were evaluated using HeLa cells and isolated mouse kidney tissue. The thick sample was scanned layer by layer in the Gaussian mode, however, in the Airy mode, the three-dimensional (3D) structure information was projected onto a two-dimensional (2D) image, which vastly increased the volume imaging speed. To show the characteristics of the Airy microscope, we performed dynamic volumetric imaging on the isolated mouse kidney tissue with two-photon.

scholarly journals Gender- and Age-dependent Changes in Kidney Androgen Protein mRNA Expression in a Knockout Mouse Model for Nephrolithiasis

2002 ◽  
Vol 50 (12) ◽  
pp. 1663-1669 ◽  
Author(s):  
Eleni G. Tzortzaki ◽  
Dayna Glass ◽  
Min Yang ◽  
Andrew P. Evan ◽  
Sharon B. Bledsoe ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Kidney Tissue ◽  
Mouse Kidney ◽  
Male Mice ◽  
Wild Type ◽  
Rt Pcr ◽  
Cytoplasmic Staining ◽  
Female Mice ◽  
Age Dependent ◽  
Wild Type Male

Kidney androgen-regulated protein (Kap) is the most abundant protein in the mouse kidney, but its function is unknown. We previously observed a significant decrease in Kap mRNA expression in whole kidney tissue from male mice with adenine phosphoribosyltransferase (APRT) deficiency and 2,8-dihydroxyadenine (DHA) nephrolithiasis. The disease phenotype is more severe in male mice and is age-dependent. To identify the cellular basis for differential Kap expression, we used in situ hybridization (ISH) and reverse transcription-polymerase chain reaction ISH (RT-PCR ISH) to identify the cell types expressing this mRNA in paraffin-embedded kidney sections. In 1-month-old wild-type male mice, Kap was detected primarily in S3 proximal tubule segments, but expression was very low in female mice. In 1-month-old APRT-deficient male mice, Kap expression was decreased significantly and was undetectable in female mice. Kap mRNA was not detected in 3- or 6-month-old mice using our standard ISH protocol, but we observed intense cytoplasmic staining in S3 proximal tubules in wild-type male mice of these age groups using an improved RT-PCR ISH procedure. Our studies demonstrate age-, gender-, and APRT genotype-dependent changes in Kap mRNA expression in mouse kidney. Kap expression is under multihormonal control, and hormonal changes in DHA-induced nephrolithiasis may account for the decreased Kap expression in APRT-deficient mice.

A novel binary matrix consisting of graphene oxide and caffeic acid for the analysis of scutellarin and its metabolites in mouse kidney by MALDI imaging

The Analyst ◽  
2021 ◽  
Vol 146 (1) ◽  
pp. 289-295
Author(s):  
Tao Wang ◽  
Hin Kiu Lee ◽  
Grace Gar Lee Yue ◽  
Arthur Chi Kong Chung ◽  
Clara Bik San Lau ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Caffeic Acid ◽  
Kidney Tissue ◽  
Mouse Kidney ◽  
Maldi Imaging ◽  
Binary Matrix

In situ localization of scutellarin and its metabolites in kidney tissue using MALDI-MSI technique with a new binary matrix mixture of graphene oxide/caffeic acid.

EFFECTS OF CISPLATIN ON ELEMENT DISTRIBUTION IN MOUSE KIDNEY TISSUE ASSAYED BY MULTIVARIATE STATISTICAL ANALYSIS

1996 ◽  
Vol 06 (01n02) ◽  
pp. 409-414 ◽  
Author(s):  
NIANQING LIU ◽  
YUE DENG ◽  
JUXIANG PAN ◽  
YINGRONG WU ◽  
CHUANGNONG ZHOU
Keyword(s):  
Statistical Analysis ◽  
Multivariate Statistical Analysis ◽  
Kidney Tissue ◽  
Element Distribution ◽  
Mouse Kidney ◽  
Cancer Drug ◽  
Multivariate Statistical ◽  
Scanning Technique ◽  
Anti Cancer

The micro-SXRF scanning technique (size 150×150µ m 2) has been used to obtain the positional distributions of Zn, Cu, Fe, Mn, Br, Rb, K, Ca, Cl, S and Pt in cryo-section of the kidneys of normal mice treated with the anti-cancer drug cisplatin. The effect of cisplatin on element correlation was assayed by multivariate statistical analysis. The results of these examination imply that the elements affected by the cisplatin treatment are Zn and Fe.

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