A novel binary matrix consisting of graphene oxide and caffeic acid for the analysis of scutellarin and its metabolites in mouse kidney by MALDI imaging

The Analyst ◽  
2021 ◽  
Vol 146 (1) ◽  
pp. 289-295
Author(s):  
Tao Wang ◽  
Hin Kiu Lee ◽  
Grace Gar Lee Yue ◽  
Arthur Chi Kong Chung ◽  
Clara Bik San Lau ◽  
...  

In situ localization of scutellarin and its metabolites in kidney tissue using MALDI-MSI technique with a new binary matrix mixture of graphene oxide/caffeic acid.

2020 ◽  
Vol 35 (2) ◽  
pp. 221-228
Author(s):  
S.-B. Chen ◽  
T.-X. Li ◽  
S.-H. Wan ◽  
X. Huang ◽  
S.-W. Cai ◽  
...  

ACS Omega ◽  
2020 ◽  
Vol 5 (49) ◽  
pp. 31535-31542
Author(s):  
Weijun Miao ◽  
Feng Wu ◽  
Shiman Zhou ◽  
Guibin Yao ◽  
Yiguo Li ◽  
...  

Pathobiology ◽  
2021 ◽  
pp. 1-12
Author(s):  
Ying Xie ◽  
Yuanyuan Ruan ◽  
Huimei Zou ◽  
Yixin Wang ◽  
Xin Wu ◽  
...  

<b><i>Objective:</i></b> The goal of the present study was to determine the expression of yes-associated protein 1 (YAP1) in renal tissues of mice with lupus nephritis (LN) and elucidate its role in the progression of renal fibrosis. <b><i>Methods:</i></b> C57BL/6 mice and MRL/lpr mice were selected for experimental comparison. Mouse kidney tissues were removed and sectioned for hematoxylin and eosin staining, Masson’s trichome staining, Sirius staining, and immunohistochemistry. The mRNA and protein levels of YAP1 in mouse kidney tissues were detected, and the correlation between YAP1 and fibronectin (FN) mRNA levels was analyzed. Mouse renal epithelial cells were used for in vitro experiments. After transfection and stimulation, the cells were divided into 4 groups, namely the C57BL/6 serum group (group 1), the MRL/lpr serum group (group 2), the MRL/lpr serum + siRNA-negative control group (group 3), and the MRL/lpr serum + siRNA-YAP1 group (group 4). Epithelial-mesenchymal transition (EMT) markers in each group were detected by Western blotting and immunofluorescence staining. Serum creatinine, blood urea nitrogen, and urinary protein levels were detected and assessed for their correlation with YAP1 mRNA levels by Spearman’s analysis. <b><i>Results:</i></b> Compared to C57BL/6 mice, MRL/lpr mice exhibited obvious changes in fibrosis in renal tissues. In addition, YAP1 expression was significantly higher in the renal tissues of MRL/lpr mice than in those of C57BL/6 mice, and YAP1 mRNA levels were positively correlated with those of FN. YAP1 silencing in lupus serum-stimulated cells could effectively relieve serum-induced EMT. Finally, we observed that YAP1 mRNA levels in mouse kidney tissue were significantly and positively correlated with the degree of renal function injury. <b><i>Conclusion:</i></b> YAP1 expression in the kidney tissues of LN mice was higher than that observed in normal mice, indicating that YAP1 may play an important role in the occurrence and development of LN.


2021 ◽  
Author(s):  
Gopal Avashthi ◽  
Man Singh

Ultrasonochemically driven graphene oxide (GrO) functionalization (f) with Sulfanilamide (SA) near-edge catalyzed heterogeneous graphene oxide (h-GrO) as economic scalable f-(SA)GrO is reported. The novel in-situ H2O association was subsequently aligned...


2020 ◽  
Vol 59 (1) ◽  
pp. 477-487 ◽  
Author(s):  
Zhuang Liu ◽  
Haiyang Fu ◽  
Bo Gao ◽  
Yixuan Wang ◽  
Kui Li ◽  
...  

AbstractThis paper studies in-situ synthesis of Fe2O3/reduced graphene oxide (rGO) anode materials by different hydrothermal process.Scanning Electron Microscopy (SEM) analysis has found that different processes can control the morphology of graphene and Fe2O3. The morphologies of Fe2O3 prepared by the hydrothermal in-situ and oleic acid-assisted hydrothermal in-situ methods are mainly composed of fine spheres, while PVP assists The thermal in-situ law presents porous ellipsoids. Graphene exhibits typical folds and small lumps. X-ray diffraction analysis (XRD) analysis results show that Fe2O3/reduced graphene oxide (rGO) is generated in different ways. Also, the material has good crystallinity, and the crystal form of the iron oxide has not been changed after adding GO. It has been reduced, and a characteristic peak appears around 25°, indicating that a large amount of reduced graphene exists. The results of the electrochemical performance tests have found that the active materials prepared in different processes have different effects on the cycle performance of lithium ion batteries. By comprehensive comparison for these three processes, the electro-chemical performance of the Fe2O3/rGO prepared by the oleic acid-assisted hydrothermal method is best.


Author(s):  
Amun Amri ◽  
Ahmad Ainun Najib ◽  
Monita Olivia ◽  
Mohammednoor Altarawneh ◽  
Aman Syam ◽  
...  

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