The alpha-subunit of glycoprotein hormones exists in the prolactin secretory granules of the bullforg (Rana catesbeiana) pituitary gland

1992 ◽  
Vol 267 (2) ◽  
pp. 223-231 ◽  
Author(s):  
Shigeyasu Tanaka ◽  
Fumihiko Mizutani ◽  
Kazutoshi Yamamoto ◽  
Saka� Kikuyama ◽  
Kazumasa Kurosumi
Keyword(s):  
Pituitary Gland ◽  
Rana Catesbeiana ◽  
Secretory Granules ◽  
Alpha Subunit ◽  
Glycoprotein Hormones

Validation of highly specific and sensitive radioimmunoassays for lutropin, follitropin, and free alpha subunit in unextracted urine

1990 ◽  
Vol 36 (2) ◽  
pp. 340-344 ◽  
Author(s):  
H Landy ◽  
A L Schneyer ◽  
R W Whitcomb ◽  
W F Crowley
Keyword(s):  
Monoclonal Antibody ◽  
Urinary Excretion ◽  
Blood Volume ◽  
High Sensitivity ◽  
High Specificity ◽  
Urine Samples ◽  
Alpha Subunit ◽  
Glycoprotein Hormones ◽  
Urinary Ph ◽  
Freeze Thaw

Abstract Measurement of the urinary excretion of lutropin (LH) and follitropin (FSH) and their common free alpha subunit (FAS) assists in monitoring the maturation of the hypothalamic-pituitary-gonadal axis and in understanding the physiology of the pituitary glycoprotein hormones. Here we describe sensitive, specific polyclonal radioimmunoassays for LH and FSH and a monoclonal radioimmunoassay for FAS for use with urine--assays unperturbed by alterations in urinary pH or osmolarity within the broad physiological range encountered in urine. Concordance between LH, FSH, and FAS concentrations in extracted and unextracted urine samples was high. Linearity and parallelism with the standard curves was observed with addition of 25 to 200 microL of unextracted urine. No effect on glycoprotein concentration was seen after as many as 10 freeze-thaw cycles. The need for extraction was further obviated by the high sensitivity of each assay, reflected by minimum detectable doses well below the concentrations encountered in patients' samples. Thus we have measured gonadotropins in unextracted urine as precisely as in extracted urine. We also have demonstrated an equally versatile assay for urinary alpha subunit, using a monoclonal antibody of high specificity for this monomer in its free, uncombined form. These radioimmunoassays complement assays of gonadotropins and free alpha subunit in serum and will allow longitudinal investigations otherwise limited by the constraints of the patient's blood volume.

Nucleotide sequence of cloned complementary deoxyribonucleic acid for the .alpha. subunit of bovine pituitary glycoprotein hormones

Biochemistry ◽  
1983 ◽  
Vol 22 (20) ◽  
pp. 4856-4860 ◽  
Author(s):  
Christopher R. Erwin ◽  
Michelle L. Croyle ◽  
John E. Donelson ◽  
Richard A. Maurer
Keyword(s):  
Nucleotide Sequence ◽  
Deoxyribonucleic Acid ◽  
Alpha Subunit ◽  
Glycoprotein Hormones ◽  
Complementary Deoxyribonucleic Acid

Ectopic production in serum-free media of the common alpha subunit of the glycoprotein hormones

In Vitro ◽  
1981 ◽  
Vol 17 (5) ◽  
pp. 421-426 ◽  
Author(s):  
Jay S. Morrow ◽  
Bruce C. Weintraub ◽  
Saul W. Rosen
Keyword(s):  
Alpha Subunit ◽  
Glycoprotein Hormones ◽  
Serum Free ◽  
Ectopic Production ◽  
The Common ◽  
Free Media

Existence of noradrenalin cells and serotonin cells in the pituitary gland of Rana catesbeiana

1983 ◽  
Vol 228 (2) ◽  
Author(s):  
Y. Kondo ◽  
I. Nagatsu ◽  
M. Yoshida ◽  
N. Karasawa ◽  
T. Nagatsu
Keyword(s):  
Pituitary Gland ◽  
Rana Catesbeiana ◽  
Serotonin Cells

scholarly journals Diabetes and Hypercholesterolemia Impair the Cytological Structure of the Anterior Pituitary Gland

2019 ◽  
Vol 16 (3) ◽  
pp. 649-658
Author(s):  
Ahmed MR Abdo ◽  
Mohamed E El-Beeh ◽  
Sameer H. Qari ◽  
Dina A El-badry ◽  
Hassan IH El-Sayyad
Keyword(s):  
Body Weight ◽  
Pituitary Gland ◽  
Anterior Pituitary ◽  
Secretory Granules ◽  
Reproductive Function ◽  
Anterior Lobe ◽  
Anterior Pituitary Gland ◽  
Albino Rats ◽  
Pituitary Glands ◽  
Cytological Picture

Increase consumption of high fat diet was found to alter blood sugar level similar to diabetes and contributed to the development of obesity and affected the reproductive function of both sexes. The study aimed to clarify the influence of diabetes and or hypercholesterolemia on the cytological picture of cells of the anterior lobe of pituitary gland of male albino rats. Eighteen male albino rats weighing approximately 120 gram body weight were divided into three main groups; control, diabetes, hypercholesterolemia, diabetes (single i.p. 40 mg streptozotocin/kg B.wt plus 100mg. nicotinamide /kg body weight) and hypercholesterolemia (diet containing 3% cholesterol). Dietary feeding on cholesterol and diabetes were carried out for 12 weeks. At the end of treatment, animals were sacrificed, and pituitary glands were separated and their anterior lobe was processed for cytological investigations by transmission electron microscopy. The present study revealed that the rats subjected to experimental diabetes and/ or hypercholesterolemia exhibited a decrease of the secretory granules within the gonadotroph cells somatotroph and corticotrophin cells. There was a detected intracellular accumulation of fat globules in both the gonado- and sommatotroph cells. The authors reported that the altered cytological structures of the secretory function of the anterior pituitary gland led to marked impairment of the male hormonal level and causing infertility.

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