Microtubule-associated distribution of specific granules and secretion of atrial natriuretic factor in primary cultures of rat cardiomyocytes

1993 ◽  
Vol 272 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Terje H. Larsen ◽  
Henrik S. Huitfeldt ◽  
Ole Myking ◽  
Thorvald S�tersdal
Keyword(s):  
Atrial Natriuretic Factor ◽  
Primary Cultures ◽  
Rat Cardiomyocytes ◽  
Natriuretic Factor ◽  
Specific Granules ◽  
Atrial Natriuretic

Plasma Atrial Natriuretic Factor and Ultrastructure of Atrial Specific Granules Following Chronic Hypoxia in Rats

1987 ◽  
Vol 73 (s17) ◽  
pp. 26P-26P ◽  
Author(s):  
R J D Winter ◽  
L Meleagros ◽  
S Pervez ◽  
T Krausz ◽  
J M Polak ◽  
...  
Keyword(s):  
Atrial Natriuretic Factor ◽  
Chronic Hypoxia ◽  
Natriuretic Factor ◽  
Specific Granules ◽  
Atrial Specific Granules ◽  
Atrial Natriuretic

Post-translational processing of atrial natriuretic factor by adult rat atrial cardiocytes in culture

1993 ◽  
Vol 71 (7) ◽  
pp. 497-505 ◽  
Author(s):  
Gilles R. Dubé ◽  
Mercedes L. Kuroski - de Bold ◽  
Adolfo J. de Bold
Keyword(s):  
Molecular Weight ◽  
Atrial Natriuretic Factor ◽  
Average Rate ◽  
Primary Cultures ◽  
Low Molecular Weight ◽  
Nuclear Staining ◽  
Adult Rat ◽  
Natriuretic Factor ◽  
Atrial Cardiocytes ◽  
Atrial Natriuretic

Post-translational processing of the cardiac polypeptide hormone atrial natriuretic factor (ANF) was studied using primary cultures of cardiocytes derived from adult rat atria. Atrial cardiocytes attached to microcarrier beads were maintained for up to 15 days under continuous superfusion in minichromatographic columns. The cultures were characterized for their ability to store, process, and release ANF and by immunofluorescence microscopy for ANF, desmin, and myosin. Nuclear staining using the fluorescent DNA stain Hoechst 33258 was carried out to determine the total number of cells in culture. Column eluates were assayed for ANF by radioimmunoassay and analyzed by reverse phase high-performance liquid chromatography. For comparison purposes, superfusion experiments using freshly isolated cardiocytes supported in Bio-Gel P2 were carried out. Freshly isolated atrial cardiocytes stored high molecular weight ANF (5.2 ± 1.9 pmol/μg DNA) and released mostly (83.3 ± 6.7%) low molecular weight ANF, at an average rate of 97 ± 18 fmol∙min−1∙μg−1 DNA. The cell content and the rate of release of ANF after 15 days in culture were 1.3 ± 0.4 pmoi/μg DNA and 1.7 ± 0.4 fmol∙min−1∙μg−1 DNA, respectively, and 62.7 ± 6.3% of the released peptide was of a low molecular weight. There was no correlation between changes in cell population and the extent of processing. Cultures of noncardiocytes, superfused with exogenous proANF, did not significantly process proANF to a lower molecular weight peptide. The present investigation shows that adult rat atrial cardiocytes, maintained superfused in microcarrier culture and in a serum-supplemented medium for up to 15 days, retain phenotypic and biochemical characteristics normally associated with the dual contractile–endocrine nature of mammalian atrial cardiocytes in vivo. The results obtained in the present work strongly support the view that ANF post-translational processing is an intrinsic property of the atrial cardiocytes and is independent of any other cell type.Key words: atrial natriuretic factor, post-translation processing, cardyocytes, adult rats, cell cultures.

Effect of a purified atrial natriuretic factor on rat and rabbit vascular strips and vascular beds

1984 ◽  
Vol 247 (1) ◽  
pp. R34-R39 ◽  
Author(s):  
R. Garcia ◽  
G. Thibault ◽  
M. Cantin ◽  
J. Genest
Keyword(s):  
Angiotensin Ii ◽  
Atrial Natriuretic Factor ◽  
Perfusion Pressure ◽  
Rat Kidney ◽  
Relaxant Effect ◽  
Natriuretic Factor ◽  
Specific Granules ◽  
Vascular Beds ◽  
Dose Dependent ◽  
Atrial Natriuretic

Rat atrium cardiocytes contain a powerful natriuretic and diuretic peptide that has been localized in the specific granules. This atrial natriuretic factor (ANF) produced a potent, dose-dependent relaxant effect on rabbit and rat arterial strips previously made to contract by application of either norepinephrine (NE) or angiotensin II. The effect was not seen if KCl was used as contractile agent or under any conditions with rabbit mesenteric strips. After the application of ANF the vascular strips were refractory to subsequent stimulation by either NE or angiotensin II. The infusion of ANF into a high-resistance isolated perfused rat kidney produced a rapid decrease (33 +/- 5 mmHg) in perfusion pressure that lasted for 18 +/- 3 min. This effect was not seen in the isolated rat mesenteric arterial preparation, even when the perfusion pressure was raised by the infusion of NE. These effects of ANF on vascular smooth muscle are not mediated by prostaglandins, by alpha- and beta-adrenergic and muscarinic receptors, or by an impairment of Ca2+ influx, but they are mimicked by sodium nitroprusside. A low- and a high-molecular-weight ANF produced the same effects. The existence of specific receptive sites for these peptides is suggested.

Atrial Natriuretic Factor Receptors

1987 ◽  
Vol 16 (1) ◽  
pp. 63-77 ◽  
Author(s):  
John W. Jacobs ◽  
George P. Vlasuk ◽  
Michael Rosenblatt
Keyword(s):  
Atrial Natriuretic Factor ◽  
Natriuretic Factor ◽  
Atrial Natriuretic

Acute and chronic sodium loading stimulates atrial natriuretic factor (ANF) in man

1986 ◽  
Vol 113 (1_Suppl) ◽  
pp. S165-S166 ◽  
Author(s):  
G. WAMBACH ◽  
S. GÖTZ ◽  
G. SUCKAU ◽  
G. BÖNNER ◽  
W. KAUFMANN
Keyword(s):  
Atrial Natriuretic Factor ◽  
Natriuretic Factor ◽  
Sodium Loading ◽  
Atrial Natriuretic
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