scholarly journals Influence of hypertension on serum concentration of type IV collagen antigens in streptozotocin-diabetic and non-diabetic rats

Diabetologia ◽  
1987 ◽  
Vol 30 (5) ◽  
pp. 344-347 ◽  
Author(s):  
C. Hasslacher ◽  
D. Brocks ◽  
J. Mann ◽  
G. Mall ◽  
R. Waldherr
Keyword(s):  
Serum Concentration ◽  
Type Iv Collagen ◽  
Diabetic Rats ◽  
Type Iv

SERUM CONCENTRATION OF TYPE IV COLLAGEN IN UROLOGICAL CANCER

1993 ◽  
Vol 84 (3) ◽  
pp. 457-462
Author(s):  
Hideyuki Isobe ◽  
Shinji Iwata ◽  
Makoto Fujime ◽  
Ryuichi Kitagawa
Keyword(s):  
Serum Concentration ◽  
Type Iv Collagen ◽  
Urological Cancer ◽  
Type Iv

Serum Concentration of Type IV Collagen 7S Domain as a Marker for Increased Risk of Recurrence after Liver Resection for Hepatocellular Carcinoma

2003 ◽  
Vol 20 (3) ◽  
pp. 201-208 ◽  
Author(s):  
Shuichi Kawai ◽  
Shoji Kubo ◽  
Tadashi Tsukamoto ◽  
Hiromu Tanaka ◽  
Taichi Shuto ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Resection ◽  
Serum Concentration ◽  
Type Iv Collagen ◽  
Risk Of Recurrence ◽  
Type Iv ◽  
Increased Risk ◽  
Type Iv Collagen 7S

scholarly journals Glomerular Basement Membrane Selective Permeability in Short-term Streptozotocin-induced Diabetic Rats

2000 ◽  
Vol 1 (1) ◽  
pp. 19-30 ◽  
Author(s):  
Michele Doucet ◽  
Irene Londoño ◽  
Amparo Gómez-Pascual ◽  
Moise Bendayan
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Type Iv Collagen ◽  
Serum Proteins ◽  
Diabetic Rats ◽  
Glomerular Permeability ◽  
Structural Alterations ◽  
Early Stages ◽  
Type Iv ◽  
Basement Membrane Thickening

In diabetes, the glomerular basement membrane undergoes thickening and structural alterations with loss of glomerular permselectivity properties. However, the onset of the alterations at early phases of diabetes is unclear. Aiming to determine the functional and structural alterations of the glomerular wall in the early stages of diabetes, we have studied the distribution of endogenous circulating albumin and type IV collagen in the glomerular basement membrane, using the immunocytochemical approach. The streptozotocin-injected hyperglycemic rat was our animal model. Renal tissues were examined after 10 days, 2, 4 and 6 months of hyperglycemia. Upon immunogold labelings, changes in the glomerular permeability to endogenous albumin were found altered as early as upon ten days of hyperglycemia. In contrast, no structural modifications were detected at this time point. Indeed, glomerular basement membrane thickening and an altered type IV collagen labeling distribution were only observed after four months of hyperglycemia, suggesting that functional alterations take place early in diabetes prior to any structural modification. In order to evaluate the reversibility of the glomerular alterations, two-month-old diabetic animals were treated with insulin. These animals showed a significant restoring of their glomerular permselectivity. Our results suggest a link between glycemic levels and alteration of glomerular permeability in early stages of diabetes, probably through high levels of glycated serum proteins.

scholarly journals Tinospora cordifoliaconsumption ameliorates changes in kidney chondroitin sulphate/dermatan sulphate in diabetic rats

2012 ◽  
Vol 1 ◽  
Author(s):  
Darukeshwara Joladarashi ◽  
Nandini D. Chilkunda ◽  
Paramahans V. Salimath
Keyword(s):  
Type Iv Collagen ◽  
Chondroitin Sulphate ◽  
Rat Kidney ◽  
Apparent Molecular Weight ◽  
Diabetic Rats ◽  
Tinospora Cordifolia ◽  
Diabetic Rat ◽  
Composition Analysis ◽  
Dermatan Sulphate ◽  
Type Iv

AbstractDiabetes is known to alter kidney extracellular matrix (ECM) components. Chondroitin sulphate (CS)/dermatan sulphate (DS), an ECM component, which plays an essential role in kidney is altered during diabetes. The focus of this study has been to examine the effect ofTinospora cordifolia(TC) consumption, a potent plant widely used to treat diabetes, on kidney CS/DS. Experimentally induced diabetic rats were fed with diet containingTCat 2·5 and 5 % levels and the effect of it on kidney CS/DS was examined. The CS/DS content and CS:heparan sulphate ratio which was decreased during diabetic condition were ameliorated inTC-fed groups. Disaccharide composition analysis of CS/DS by HPLC showed that decreases in ‘E’ units and degree of sulphation were modulated in 5 %TC-fed groups. Apparent molecular weight of purified CS/DS from the control rat kidney was found to be 38 kDa which was decreased to 29 kDa in diabetic rat kidney. Rats in 5 %TC-fed groups showed chain length of 38 kDa akin to control rats. Expression of chondroitin 4-O-sulfotransferase-1, dermatan 4-O-sulfotransferase-1 andN-acetylgalactosamine 4 sulphate 6-O-sulfotransferase, enzymes involved in the synthesis of ‘E’ units which was reduced during diabetic condition, was significantly contained in the 5 %TC-fed group. Purified CS/DS from 5 %TC-fed group was able to bind higher amounts of ECM components, namely type IV collagen and laminin, when compared with untreated diabetic rats. The present results demonstrate that consumption of a diet containingTCat the 5 % level modulates changes in kidney CS/DS which were due to diabetes.

Ethyl pyruvate ameliorates albuminuria and glomerular injury in the animal model of diabetic nephropathy

2012 ◽  
Vol 302 (5) ◽  
pp. F606-F613 ◽  
Author(s):  
Kyung Don Ju ◽  
Eun Kyoung Shin ◽  
Eun Jin Cho ◽  
Hyun Bae Yoon ◽  
Hyo Sang Kim ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Nadph Oxidase ◽  
Type Iv Collagen ◽  
Transforming Growth Factor ◽  
Ethyl Pyruvate ◽  
Reactive Oxygen Species Generation ◽  
Diabetic Rats ◽  
Protective Mechanism ◽  
Type Iv

Pyruvate is an endogenous antioxidant and anti-inflammatory substance. The present study was implemented to investigate the protective effect of ethyl pyruvate (EP) against the development and progression of diabetic nephropathy in an in vivo and in vitro model. Diabetic rats were prepared by injecting streptozotocin (65 mg/kg). Those that developed diabetes after 72 h were treated with EP (40 mg/kg) intraperitoneally. Diabetic rats without pyruvate treatment and nondiabetic rats were used for control. As an in vitro experiment, rat mesangial cells cultured primarily from Sprague-Dawley rats were treated in high-glucose (HG; 50 mM) or normal-glucose (NG; 5 mM) conditions and with or without pyruvate. Pyruvate-treated diabetic rats exhibited decreased albuminuria and attenuated NADPH-dependent reactive oxygen species generation. Immunohistochemistry showed reduced laminin, type IV collagen, and fibronectin deposition in the glomeruli compared with nontreated diabetic rats. Parallel changes were shown in tissue mRNA and protein expression levels of monocyte chemoattractant protein-1, transforming growth factor-β1, laminin, fibronectin, and type IV collagen in the kidney. Concordantly, protective effects were also exhibited in the mesangial cell culture system. These findings suggest that pyruvate protects against kidney injury via NADPH oxidase inhibition. The present study established that activation of NADPH oxidase plays a crucial role in diabetes-induced oxidative stress, glomerular hypertrophy, and ECM molecule expression. Pyruvate exhibited a renoprotective effect in the progression of experimental diabetic nephropathy. Future research is warranted to investigate the protective mechanism of pyruvate more specifically in relation to NADPH oxidase in diabetic nephropathy.

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