Chloroplast DNA codes for the ribulose diphosphate carboxylase catalytic site on fraction I proteins of Nicotiana species

1973 ◽  
Vol 124 (3) ◽  
pp. 187-196 ◽  
Author(s):  
Shalini Singh ◽  
S. G. Wildman
Keyword(s):  
Chloroplast Dna ◽  
Catalytic Site ◽  
Nicotiana Species ◽  
Dna Codes ◽  
Fraction I ◽  
Ribulose Diphosphate Carboxylase

Beta chloroplast genomes: analysis of Fraction I protein and chloroplast DNA variation

1987 ◽  
Vol 73 (3) ◽  
pp. 330-336 ◽  
Author(s):  
Y. Kishima ◽  
T. Mikami ◽  
A. Hirai ◽  
M. Sugiura ◽  
T. Kinoshita
Keyword(s):  
Chloroplast Dna ◽  
Fraction I Protein ◽  
Dna Variation ◽  
Chloroplast Genomes ◽  
Chloroplast Dna Variation ◽  
Fraction I

scholarly journals Chloroplast DNA codes for transfer RNA

1976 ◽  
Vol 3 (8) ◽  
pp. 2005-2018 ◽  
Author(s):  
J. M. McCrea ◽  
C. L. Hershberger
Keyword(s):  
Chloroplast Dna ◽  
Transfer Rna ◽  
Dna Codes

Chloroplast DNA codes for tRNA from cytoplasmic polyribosomes

Nature ◽  
1978 ◽  
Vol 274 (5672) ◽  
pp. 717-719 ◽  
Author(s):  
JAN MILLER McCREA ◽  
CHARLES L. HERSHBERGER
Keyword(s):  
Chloroplast Dna ◽  
Dna Codes

Nucleinsäuren in Chloroplasten I. Charakterisierung der DNS und RNS von Antirrhinum majus

1967 ◽  
Vol 22 (10) ◽  
pp. 1068-1076 ◽  
Author(s):  
Hans Georg Ruppel
Keyword(s):  
Nucleic Acids ◽  
Chloroplast Dna ◽  
Density Gradient ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Size Fraction ◽  
Sedimentation Coefficient ◽  
Antirrhinum Majus ◽  
Freeze Dried ◽  
Fraction I

The occurrence of nucleic acids in chloroplast preparations and leaves of Antirrhinum majus has been investigated.Chloroplast preparations isolated from freeze-dried leaves in a non-aqueous medium were found to contain DNA. Characterization of the DNA was based on chemical analyses and density gradient centrifugation. The chloroplast DNA has a density of 1.698 g/cm3 corresponding to a guaninecytosine content of 37 — 38%. The hyperchromic effect of the DNA has shown its double-stranded structure. Leaf DNA has also been characterized by density gradient centrifugation and shown to contain as major component the nuclear DNA with a density of 1.689 g/cm3 which corresponds to a guanine-cytosine content of about 31% and a minor component identified as chloroplast DNA.Extraction of the total nucleic acids of chloroplast preparations and their chromatographic separation on methylated serum albumin column yielded 5 fractions as follows: Soluble RNA (fraction I) with s=3.8—4.0, DNA (fraction II) containing small but detectable amounts of an unknown RNA component, two RNA fractions presumable of ribosomal origin with s = 17 (fraction III) and s = 26 (fraction IV), and a RNA fraction heterogeneous in molecular size (fraction V) with s=23 — 50. The soluble RNA has a high GC-content whereas fraction V shows a relative high adenine content. Compared with the nucleic acids from leaves the chloroplast preparations contain the same fractions, differing only in the higher content of fraction III related to the amount of fraction IV. Beside the DNA fraction of leaf extract with the sedimentation coefficient of s = 12 — 13 a further UV-absorbing component with s=5.0—5.5 has been found. Its character is unknown.

scholarly journals Subunits From Reduced and S-Carboxymethylated Ribulose Diphosphate Carboxylase (Fraction I Protein)

1969 ◽  
Vol 22 (2) ◽  
pp. 463 ◽  
Author(s):  
KE Moon ◽  
EOP Thompson
Keyword(s):  
Ammonium Sulphate ◽  
Gel Filtration ◽  
Fraction I Protein ◽  
Ammonium Sulphate Fractionation ◽  
Fraction I ◽  
Ribulose Diphosphate Carboxylase

Fraction I protein isolated from spinach beet chloroplasts was purified by ammonium sulphate fractionation and Sephadex G�200 gel filtration. The isolated protein was reduced and S-carboxymethylated, and the dissociated protein resolved into two distinct subunits by gel filtration on Sephadex G-200 in an 8M urea buffer at pH 10�0.

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