Ultrastructure of the Y chromosomal lampbrush loops in primary spermatocytes of Drosophila hydei

Chromosoma ◽  
1984 ◽  
Vol 89 (2) ◽  
pp. 85-95 ◽  
Author(s):  
Caspar J. Grond ◽  
Rob G. J. Rutten ◽  
Wolfgang Hennig
Keyword(s):  
Drosophila Hydei ◽  
Lampbrush Loops

DNA clones and RNA transcripts of four lampbrush loops from the Y chromosome of Drosophila hydei

Cell ◽  
1983 ◽  
Vol 32 (1) ◽  
pp. 191-199 ◽  
Author(s):  
Eliezer Lifschytz ◽  
Dana Hareven ◽  
Aviva Azriel ◽  
Howard Brodsly
Keyword(s):  
Y Chromosome ◽  
Rna Transcripts ◽  
Drosophila Hydei ◽  
Lampbrush Loops

scholarly journals Mega-introns in the Dynein Gene DhDhc7(Y) on the Heterochromatic Y Chromosome Give Rise to the Giant Threads Loops in Primary Spermatocytes of Drosophila hydei

Genetics ◽  
2000 ◽  
Vol 154 (2) ◽  
pp. 759-769 ◽  
Author(s):  
Alexander M Reugels ◽  
Roman Kurek ◽  
Ulrich Lammermann ◽  
Hans Bünemann
Keyword(s):  
Satellite Dna ◽  
Transcription Unit ◽  
Drosophila Hydei ◽  
Y Chromosomes ◽  
Protein Encoding ◽  
Species Specific ◽  
Giant Size ◽  
Large Clusters ◽  
Fertility Genes ◽  
Lampbrush Loops

Abstract The heterochromatic Y chromosomes of several Drosophila species harbor a small number of male fertility genes (fertility factors) with several unusual features. Expression of their megabase-sized loci is restricted to primary spermatocytes and correlates with the unfolding of species-specific lampbrush loop-like structures resulting from huge transcripts mainly derived from clusters of loop-specific Y chromosomal satellites. Otherwise, there is evidence from genetic mapping and biochemical experiments that at least two of these loops, Threads in Drosophila hydei and kl-5 in D. melanogaster, colocalize with the genes for the axonemal dynein β heavy chain proteins DhDhc7(Y) and Dhc-Yh3, respectively. Here, we make use of particular Threads mutants with megabase-sized deletions for direct mapping of DhDhc7(Y)-specific exons among the large clusters of satellite DNA within the 5.1-Mb Threads transcription unit. PCR experiments with exon-specific primer pairs, in combination with hybridization experiments with exon- and satellite-specific probes on filters with large PFGE-generated DNA fragments, offer a simple solution for the long-lasting paradox between megabase-sized loops and protein-encoding transcription units; the lampbrush loops Threads and the DhDhc7(Y) gene are one and the same transcription unit, and the giant size of the DhDhc7(Y) gene as well as its appearance as a giant lampbrush loop are merely the result of transcription of huge clusters of satellite DNA within some of its 20 introns.

scholarly journals The Y Chromosomal Fertility Factor Threads in Drosophila hydei Harbors a Functional Gene Encoding an Axonemal Dynein β Heavy Chain Protein

Genetics ◽  
1998 ◽  
Vol 149 (3) ◽  
pp. 1363-1376 ◽  
Author(s):  
Roman Kurek ◽  
Alexander M Reugels ◽  
Karl Heinz Glaätzer ◽  
Hans Bünemann
Keyword(s):  
Heavy Chain ◽  
Gene Encoding ◽  
Chain Molecules ◽  
Sperm Tail ◽  
Fertility Factor ◽  
Putative Protein ◽  
Drosophila Hydei ◽  
Dynein Arms ◽  
Large Clusters ◽  
Lampbrush Loops

Abstract To understand the contradiction between megabase-sized lampbrush loops and putative protein encoding genes both associated with the loci of Y chromosomal fertility genes of Drosophila on the molecular level, we used PCR-mediated cloning to identify and isolate the cDNA sequence of the Y chromosomal Drosophila hydei gene DhDhc7(Y). Alignment of the sequences of the putative protein DhDhc7(Y) and the outer arm dynein β heavy chain protein DYH2 of Tripneustes gratilla shows homology over the entire length of the protein chains. Therefore the proteins can be assumed to fulfill orthologous functions within the sperm tail axonemes of both species. Functional dynein β heavy chain molecules, however, are necessary for the assembly and attachment of outer dynein arms within the sperm tail axoneme. Localization of DhDhc7(Y) to the fertility factor Threads, comprising at least 5.1 Mb of transcriptionally active repetitive DNA, results from an infertile Threads− mutant where large clusters of Threads specifically transcribed satellites and parts of DhDhc7(Y) encoding sequences are missing simultaneously. Consequently, the complete lack of the outer dynein arms in Threads− males most probably causes sperm immotility and hence infertility of the fly. Moreover, preliminary sequence analysis and several other features support the hypothesis that DhDhc7(Y) on the lampbrush loops Threads in D. hydei and Dhc-Yh3 on the lampbrush loops kl-5 in Drosophila melanogaster on the heterochromatic Y chromosome of both species might indeed code for orthologous dynein β heavy chain proteins.

Retrotransposon-like sequences are expressed in Y chromosomal lampbrush loops of Drosophila hydei

1988 ◽  
Vol 203 (3) ◽  
pp. 689-697 ◽  
Author(s):  
Peter Huijser ◽  
Christiane Kirchhoff ◽  
Dirk-Henner Lankenau ◽  
Wolfgang Hennig
Keyword(s):  
Drosophila Hydei ◽  
Lampbrush Loops

A group of non-Y encoded Drosophila hydei primary spermatocyte nuclear glycoproteins exhibits epitopes depending on formation of Y chromosomal giant lampbrush loops

Chromosoma ◽  
1989 ◽  
Vol 98 (2) ◽  
pp. 144-152 ◽  
Author(s):  
G. Tischendorf ◽  
W. Liebrich ◽  
P. Trapitz ◽  
G. Wood ◽  
M. Schwochau
Keyword(s):  
Primary Spermatocyte ◽  
Drosophila Hydei ◽  
Lampbrush Loops
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