Towards a physical map of the fertility genes on the heterochromatic Y chromosome of Drosophila hydei: Families of repetitive sequences transcribed on the lampbrush loops Nooses and Threads are organized in extended clusters of several hundred kilobases

1992 ◽  
Vol 235 (2-3) ◽  
pp. 221-234 ◽  
Author(s):  
Peter Trapitz ◽  
Karl Heinz Glätzer ◽  
Hans Bünemann
Keyword(s):  
Y Chromosome ◽  
Physical Map ◽  
Repetitive Sequences ◽  
Drosophila Hydei ◽  
Fertility Genes ◽  
Lampbrush Loops

DNA clones and RNA transcripts of four lampbrush loops from the Y chromosome of Drosophila hydei

Cell ◽  
1983 ◽  
Vol 32 (1) ◽  
pp. 191-199 ◽  
Author(s):  
Eliezer Lifschytz ◽  
Dana Hareven ◽  
Aviva Azriel ◽  
Howard Brodsly
Keyword(s):  
Y Chromosome ◽  
Rna Transcripts ◽  
Drosophila Hydei ◽  
Lampbrush Loops

scholarly journals Mega-introns in the Dynein Gene DhDhc7(Y) on the Heterochromatic Y Chromosome Give Rise to the Giant Threads Loops in Primary Spermatocytes of Drosophila hydei

Genetics ◽  
2000 ◽  
Vol 154 (2) ◽  
pp. 759-769 ◽  
Author(s):  
Alexander M Reugels ◽  
Roman Kurek ◽  
Ulrich Lammermann ◽  
Hans Bünemann
Keyword(s):  
Satellite Dna ◽  
Transcription Unit ◽  
Drosophila Hydei ◽  
Y Chromosomes ◽  
Protein Encoding ◽  
Species Specific ◽  
Giant Size ◽  
Large Clusters ◽  
Fertility Genes ◽  
Lampbrush Loops

Abstract The heterochromatic Y chromosomes of several Drosophila species harbor a small number of male fertility genes (fertility factors) with several unusual features. Expression of their megabase-sized loci is restricted to primary spermatocytes and correlates with the unfolding of species-specific lampbrush loop-like structures resulting from huge transcripts mainly derived from clusters of loop-specific Y chromosomal satellites. Otherwise, there is evidence from genetic mapping and biochemical experiments that at least two of these loops, Threads in Drosophila hydei and kl-5 in D. melanogaster, colocalize with the genes for the axonemal dynein β heavy chain proteins DhDhc7(Y) and Dhc-Yh3, respectively. Here, we make use of particular Threads mutants with megabase-sized deletions for direct mapping of DhDhc7(Y)-specific exons among the large clusters of satellite DNA within the 5.1-Mb Threads transcription unit. PCR experiments with exon-specific primer pairs, in combination with hybridization experiments with exon- and satellite-specific probes on filters with large PFGE-generated DNA fragments, offer a simple solution for the long-lasting paradox between megabase-sized loops and protein-encoding transcription units; the lampbrush loops Threads and the DhDhc7(Y) gene are one and the same transcription unit, and the giant size of the DhDhc7(Y) gene as well as its appearance as a giant lampbrush loop are merely the result of transcription of huge clusters of satellite DNA within some of its 20 introns.

GENETIC FINE STRUCTURE OF THE Y CHROMOSOME OF DROSOPHILA HYDEI

Genetics ◽  
1982 ◽  
Vol 101 (2) ◽  
pp. 257-277
Author(s):  
Johannes H P Hackstein ◽  
Orilio Leoncini ◽  
Heinz Beck ◽  
Gijsbert Peelen ◽  
Wolfgang Hennig
Keyword(s):  
Fine Structure ◽  
Y Chromosome ◽  
Genetic Map ◽  
Complementation Group ◽  
Male Sterile ◽  
Drosophila Hydei ◽  
Complementation Groups ◽  
Genetic Fine Structure ◽  
Lampbrush Loops ◽  
Exact Definition

ABSTRACT A genetic map of the Y chromosome of Drosophila hydei has been constructed from deletion/complementation experiments, with the aid of male sterile mutants of the Y chromosome. A central conclusion of our experiments is that not more than a single complementation group can be detected in each of the lampbrush loop forming sites. Additional complementation groups, functionally independent of lampbrush loops, reside between these loci. Six complementation groups have been defined by several methods of mapping. An additional ten complementation groups are indicated, but their exact definition requires further investigation. The "synthetic sterility" of mutations in these ten loci contributes to the difficulty in unequivocally establishing their individual boundaries. Mapping problems also arise from the instability of certain mutants.

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