Development of DNA puffs and patterns of polypeptide synthesis in the salivary glands of Bradysia hygida

Chromosoma ◽  
1984 ◽  
Vol 89 (4) ◽  
pp. 280-284 ◽  
Author(s):  
Eduardo M. Laicine ◽  
Maurílio A. R. Alves ◽  
Jorge C. de Almeida ◽  
Elizete Rizzo ◽  
Wander C. Albernaz ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Polypeptide Synthesis ◽  
Dna Puffs ◽  
Bradysia Hygida

Mechanisms of programmed cell death in the midgut and salivary glands from Bradysia hygida (Diptera: Sciaridae) during pupal–adult metamorphosis

2020 ◽  
Vol 44 (10) ◽  
pp. 1981-1990
Author(s):  
Thaylise Cassia Santos Przepiura ◽  
Aryelle M. Navarro ◽  
Rafaela Rosa Ribeiro ◽  
José R. Gomes ◽  
Karina V. Pitthan ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Salivary Glands ◽  
Bradysia Hygida

Indirect immune detection of ecdysone receptor (EcR) during the formation of DNA puffs in Bradysia hygida (Diptera, Sciaridae)

2008 ◽  
Vol 16 (4) ◽  
pp. 609-622 ◽  
Author(s):  
Juliana Aparecida Candido-Silva ◽  
Daniela Palermo de Carvalho ◽  
Graciana Rodrigues Coelho ◽  
Jorge Cury de Almeida
Keyword(s):  
Ecdysone Receptor ◽  
Dna Puffs ◽  
Immune Detection ◽  
Bradysia Hygida

scholarly journals The promoter of DNA puff gene II/9-1 of Sciara coprophila is inducible by ecdysone in late prepupal salivary glands of Drosophila melanogaster.

1991 ◽  
Vol 2 (11) ◽  
pp. 875-888 ◽  
Author(s):  
B Bienz-Tadmor ◽  
H S Smith ◽  
S A Gerbi
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Glands ◽  
Dna Amplification ◽  
P Element ◽  
Control Of Gene Expression ◽  
Evolutionarily Conserved ◽  
Dna Puffs ◽  
P Element Transformation ◽  
Transgenic Drosophila

DNA puffs occur in Sciarid salivary gland chromosomes; they are sites of DNA amplification and intense transcription and they appear to encode secreted structural proteins needed for pupation. In this report we have used P-element transformation of Drosophila to study regulation of a Sciara DNA puff gene. We found that a 718-bp promoter fragment of DNA puff gene II/9-1 from Sciara coprophila directs expression of the bacterial reporter gene CAT in late prepupal salivary glands of transgenic Drosophila melanogaster. The identical tissue and analogous stage specificity indicate that some aspects of the ecdysone response are evolutionarily conserved between Drosophila and Sciara. When transgenic salivary glands are cultured in vitro, CAT activity is rapidly induced by ecdysone, suggesting direct control of gene expression by the ecdysone receptor. Putative stage-specific factors limit expression of the chimeric Sciara-CAT gene in transgenic Drosophila to late prepupae but not to third instar larvae when ecdysone titers are also high.

The DNA puff BhB10-1 gene encodes a glycine-rich protein secreted by the late stage larval salivary glands of Bradysia hygida

Gene ◽  
1999 ◽  
Vol 231 (1-2) ◽  
pp. 67-75 ◽  
Author(s):  
A.M. Fontes ◽  
M.E. Conacci ◽  
N. Monesi ◽  
J.C. de Almeida ◽  
M.L. Paçó-Larson
Keyword(s):  
Salivary Glands ◽  
Late Stage ◽  
Glycine Rich Protein ◽  
Bradysia Hygida

The allometric relationship between nucleus and cytoplasm in the salivary glands of Bradysia hygida

1983 ◽  
Vol 61 (6) ◽  
pp. 1311-1316
Author(s):  
Heid Sueli Leme dos Santos ◽  
André Ricciardi Cruz
Keyword(s):  
Larval Stage ◽  
Polytene Chromosomes ◽  
Critical Time ◽  
Functional Changes ◽  
Allometric Relations ◽  
Fourth Larval Stage ◽  
Glandular Cells ◽  
Allometric Relation ◽  
Dna Puffs ◽  
Bradysia Hygida

The volumetric relationship between nucleus and cytoplasm of the Bradysia hygida salivary gland cells was studied during development using an allometric relation. This relationship during the fourth larval stage is defined by a simple allometric relation. Cell growth, differentiation, and maturation during the fourth larval stage takes place in two distinct steps separated by an interphase occurring between the 6th and 8th day after the third moult. This interphase marks a critical time of development and coincides with the occurrence of DNA puffs on the polytene chromosomes of the glandular cells. Although the allometric coefficient does not change over two distinct developmental steps (k = 1), our observations support the hypothesis that the interphase observed in the allometric relations is subject to the appearance of DNA puffs and is related to functional changes in the gland.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Biogenesis of Catalase-Positive Rods in Excretory Duct Cells of Salivary Glands

1976 ◽  
Vol 34 ◽  
pp. 62-63
Author(s):  
Dwight K. Romanovicz ◽  
Jacob S. Hanker
Keyword(s):  
Light Microscopy ◽  
Salivary Glands ◽  
Submandibular Gland ◽  
Excretory Duct ◽  
Exocrine Glands ◽  
Duct Cells ◽  
Peroxidatic Activity ◽  
Microscopic Studies ◽  
Different Dimensions

The presence of catalase-positive rods (Fig. 1) of different dimensions, which frequently have a crystalline appearance by light microscopy, has been reported. They seem to be related to peroxisomes which were characterized morphologically and cytochemically in parotid and other exocrine glands of the rat by Hand in 1973. Our light microscopic studies of these spherical microbodies and rods of different sizes, stained by virtue of the peroxidatic activity of their catalase, indicate that they are almost entirely confined to the cells of the striated and execretory ducts of the submandibular gland in the mouse. The rods were usually noted only in the proximity of the ductal microbodies. The latter frequently showed a tendency to appear in linear close array, or even to be contiguous (Fig. 2). This suggested that the rods could be formed by the fusion of microbodies.

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