Involvement of sulfhydryl groups of chromosomal proteins in sister chromatid differentiation

Chromosoma ◽  
1980 ◽  
Vol 77 (3) ◽  
pp. 325-332 ◽  
Author(s):  
Charles H. C. M. Buys ◽  
Stef Stienstra
Keyword(s):  
Sister Chromatid ◽  
Sulfhydryl Groups ◽  
Chromosomal Proteins ◽  
Sister Chromatid Differentiation

The influence of demethylating agents on preimplantation development of mice

Zygote ◽  
1998 ◽  
Vol 6 (4) ◽  
pp. 351-358 ◽  
Author(s):  
E.L. Patkin ◽  
M.E. Kustova ◽  
P. Perticone
Keyword(s):  
Sister Chromatid ◽  
Cell Number ◽  
Sister Chromatid Exchanges ◽  
Preimplantation Development ◽  
Preimplantation Embryos ◽  
Cell Cycles ◽  
Severe Reduction ◽  
The Mean ◽  
Sister Chromatid Differentiation ◽  
Chromatid Exchanges

The effects of two demethylating drugs with a different mechanism of action (5-azacytidine (Aza) and L-ethionine (Eth)) on mouse preimplantation development were investigated. Preimplantation embryos were cultured for 24 h in the presence of the drug and for an additional 24 or 48 h (depending on the cleavage stage) in medium supplemented with bromodeoxyuridine to reveal sister chromatid exchanges (SCEs) and the number of cell cycles performed before harvesting. Striking differences between the two drugs were observed in their influence on proliferation of blastomeres, primary differentiation and sister chromatid differentiation (SCD), and in the pattern of DNA methylation and the frequency of SCEs per cell. At a final concentration of 1 μM Aza had no effects, whereas higher concentrations stopped development of all stages except the zygote. In contrast Eth treatments (5 mM) resulted in a severe reduction of the mean cell number per embryo in comparison with controls. Moreover both the absence of blastocyst formation and no effects on mitotic activity were detected. The most prominent effect of Eth was detected at the zygote and 4-cell stages. An unexpected decrease in SCE frequency in Eth-treated morulae and 4-cell embryos has been observed. Data are explained taking into account the different mechanisms of action of the agents.

Improved sister-chromatid differentiation using paraffin-coated bromodeoxyuridine tablets in mice

1983 ◽  
Vol 119 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Alfred F. McFee ◽  
Karen W. Lowe ◽  
Juan R. San Sebastian
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Differentiation

Sister chromatid differentiation after in situ detection of ultraviolet-induced DNA breaks under electron microscopy

1994 ◽  
Vol 82 (1) ◽  
pp. 33-37
Author(s):  
Joséluis Fernández ◽  
Asunción Campos ◽  
Vicente Goyanes ◽  
Ismael Buño ◽  
Jaime Gosálvez
Keyword(s):  
Electron Microscopy ◽  
Sister Chromatid ◽  
Dna Breaks ◽  
Sister Chromatid Differentiation ◽  
In Situ Detection

scholarly journals Revised and Improved Procedure for Immunolocalization of Male Meiotic Chromosomal Proteins and Spindle in Plants without the Use of Enzymes

Plants ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 93
Author(s):  
Kuntal De ◽  
Li Yuan ◽  
Christopher Makaroff
Keyword(s):  
Arabidopsis Thaliana ◽  
Chromosome Segregation ◽  
Sister Chromatid ◽  
Model Organism ◽  
Meiotic Spindle ◽  
Chromosomal Proteins ◽  
Meiotic Chromosomes ◽  
Wide Range ◽  
Chromatid Cohesion ◽  
Spindle Structure

Immunolocalization studies to visualize the distribution of proteins on meiotic chromosomes have become an integral part of studies on meiosis in the model organism Arabidopsis thaliana. These techniques have been used to visualize a wide range of meiotic proteins involved in different aspects of meiosis, including sister chromatid cohesion, recombination, synapsis, and chromosome segregation. However, the analysis of meiotic spindle structure by immunofluorescence is of outstanding importance in plant reproductive biology and is very challenging. In the following report, we describe the complete and easy protocol for the localization of proteins to the male meiotic spindle and male meiotic chromosomes. The protocol is fast, improved, and robust without the use of any harsh enzymes.

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