Sister chromatid differentiation and chromosomal in situ suppression hybridization: a combined methodology for analyzing cell proliferation and SCEs in individual chromosomes

1992 ◽  
Vol 61 (2) ◽  
pp. 99-102 ◽  
Author(s):  
S. Knuutila ◽  
M. Larramendy
Keyword(s):  
Cell Proliferation ◽  
Sister Chromatid ◽  
Sister Chromatid Differentiation

Sister chromatid differentiation after in situ detection of ultraviolet-induced DNA breaks under electron microscopy

1994 ◽  
Vol 82 (1) ◽  
pp. 33-37
Author(s):  
Joséluis Fernández ◽  
Asunción Campos ◽  
Vicente Goyanes ◽  
Ismael Buño ◽  
Jaime Gosálvez
Keyword(s):  
Electron Microscopy ◽  
Sister Chromatid ◽  
Dna Breaks ◽  
Sister Chromatid Differentiation ◽  
In Situ Detection

Noninvasive Carcinoma of the Breast: Angiogenesis and Cell Proliferation

2004 ◽  
Vol 128 (8) ◽  
pp. 893-896 ◽  
Author(s):  
Ying Cao ◽  
Gladell P. Paner ◽  
Leonard B. Kahn ◽  
Prabha B. Rajan
Keyword(s):  
Cell Proliferation ◽  
Microvessel Density ◽  
Carcinoma In Situ ◽  
Nuclear Grade ◽  
Proliferation Index ◽  
Carcinoma Of The Breast ◽  
Cell Proliferation Index ◽  
Noninvasive Carcinoma ◽  
Mib 1

Abstract Context.—Angiogenesis and the cell proliferation index can predict the prognosis of invasive breast carcinoma; however, little is known of their roles in noninvasive tumor. Objective.—To investigate the correlation of microvessel density and cell proliferation index with other histologic parameters (histologic type, nuclear grade, and mitotic count) in 65 cases of noninvasive carcinoma of the breast. Design.—Formalin-fixed, paraffin-embedded tissues from 65 cases of carcinoma in situ of the breast were immunostained with antibody against factor VIII antigen and proliferation-associated nuclear antigen MIB-1. The microvessel density was measured by counting the total number of microvessels around the carcinoma in situ per 10 low-power microscopic fields. The cell proliferation index was calculated by counting MIB-1–positive nuclei in 100 tumor cells. A χ2 test and Spearman rank correlation test were used for statistical analysis. Results.—The microvessel density and cell proliferation index of comedo-type, high-nuclear-grade ductal carcinomas in situ are significantly higher than those of either noncomedo type ductal carcinomas in situ or lobular carcinoma in situ (P < .001). Conclusions.—Angiogenesis and the cell proliferation index are active biological processes and may be considered as markers to separate low- and high-risk patients with noninvasive breast carcinomas.

Contrasting patterns of c-myc and N-myc expression in proliferating, quiescent, and differentiating cells of the embryonic chicken lens

Development ◽  
1992 ◽  
Vol 115 (3) ◽  
pp. 813-820
Author(s):  
L.L. Harris ◽  
J.C. Talian ◽  
P.S. Zelenka
Keyword(s):  
Cell Proliferation ◽  
Protein Product ◽  
Mrna Levels ◽  
Lens Fiber ◽  
Proliferating Cells ◽  
Fiber Cells ◽  
Embryonic Chicken ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

The present study uses the polymerase chain reaction and in situ hybridization to examine c-myc and N-myc mRNA in the embryonic chicken lens at 6, 10, 14 and 19 days of development and compares the pattern of expression obtained with the developmental pattern of cell proliferation and differentiation. In the central epithelium, c-myc mRNA levels were proportional to the percentage of proliferating cells throughout development. N-myc mRNA expression in this region was relatively low and showed no correlation with cell proliferation. The ratio of N-myc to c-myc mRNA increased markedly with the onset of epithelial cell elongation and terminal fiber cell differentiation, although both c-myc and N-myc mRNAs continued to be expressed in postmitotic, elongating cells of the equatorial epithelium and in terminally differentiating lens fiber cells. Thus, increased expression of N-myc, a gene whose protein product may compete with c-myc protein for dimerization partners, accompanies the dissociation of c-myc expression and cell proliferation during terminal differentiation of lens fiber cells.

scholarly journals Cognate antigen engagement on parenchymal cells stimulates CD8+ T cell proliferation in situ

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Robyn M. Sutherland ◽  
Sarah L. Londrigan ◽  
Jamie L. Brady ◽  
Emma M. Carrington ◽  
Julia M. Marchingo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
Cd8 T Cell ◽  
T Cell Proliferation ◽  
Cognate Antigen ◽  
Parenchymal Cells

Cell Proliferation in the Endolymphatic Sac In Situ After the Rat Waldeyer Ring Equivalent Immunostimulation

2003 ◽  
Vol 113 (9) ◽  
pp. 1609-1614 ◽  
Author(s):  
Zhen Yan ◽  
Ji-Bao Wang ◽  
Shu-Sheng Gong ◽  
Xiang Huang
Keyword(s):  
Cell Proliferation ◽  
Endolymphatic Sac

scholarly journals Cell proliferation assay – method optimisation for in vivo labeling of DNA in the rat forestomach

2017 ◽  
Vol 67 (1) ◽  
pp. 1-10
Author(s):  
Gordana Joksić ◽  
Mileva Mićić ◽  
Jelena Filipović ◽  
Dunja Drakulić ◽  
Miloš Stanojlović ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Immunohistochemical Analysis ◽  
Optimal Dose ◽  
Cell Labeling ◽  
Assay Method ◽  
Proliferating Cells ◽  
Adult Rats ◽  
In Vivo Labeling

AbstractThe study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2′-deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2′-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2′-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2′-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2′-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2′-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2′-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.

The relationship between cell proliferation and the transcription of the nuclear oncogenes c-myc, c-myb and c-ets-1 during feather morphogenesis in the chick embryo

Development ◽  
1991 ◽  
Vol 111 (3) ◽  
pp. 699-713 ◽  
Author(s):  
X. Desbiens ◽  
C. Queva ◽  
T. Jaffredo ◽  
D. Stehelin ◽  
B. Vandenbunder
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Spatial Distribution ◽  
Chick Embryo ◽  
Expression Patterns ◽  
S Phase ◽  
Skin Morphogenesis ◽  
Dermal Cells ◽  
The Relationship

We have described the expression of three nuclear protooncogenes, c-myc, c-myb and c-ets-1 during feather morphogenesis in the chick embryo. In parallel with the expression patterns obtained by in situ hybridization, we have mapped the spatial distribution of S-phase cells by monitoring the incorporation of 5-bromodeoxyuridine. We do not detect c-myc or c-myb transcripts during the early stages when S-phase cells are scattered in the dermis and in the epidermis. Rather c-ets-1 transcripts are abundant in the dermal cells which divide and accumulate under the uniform epidermis. At the onset of the formation of the feather bud, cells within each rudiment cease DNA replicative activities and c-myc transcripts are detected both in the epidermis and in the underlying dermis. This expression precedes the reentry into the S phase. The transcription of c-myb, which has been previously tightly linked to hemopoietic cells is also detected in the developing skin. This expression is essentially located in proliferating epidermal cells on and after the beginning of feather outgrowth. As feather outgrowth proceeds, the distribution of c-myc and c-myb transcripts is restricted to the highly proliferating epidermis. In contrast c-ets-1 transcripts are never detected in the epidermis. During the later stages of skin morphogenesis, the transcription of c-ets-1 is restricted to the endothelial cells of blood vessels, as previously described. We suggest that the differential expression of these nuclear oncogenes reflects the activation of different mitotic controlling pathways during the development of the skin.

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