In vitro propagation and chromosome doubling of a Triticum crassum x Hordeum vulgare intergeneric hybrid

1981 ◽  
Vol 60 (2) ◽  
pp. 89-96 ◽  
Author(s):  
C. Nakamura ◽  
W. A. Keller ◽  
G. Fedak
Keyword(s):  
Hordeum Vulgare ◽  
In Vitro Propagation ◽  
Chromosome Doubling ◽  
Intergeneric Hybrid

Changes in the fertility and meiotic behaviour of barley (Hordeum vulgare) × wheat (Triticum aestivum) hybrids regenerated from tissue cultures

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 261-266 ◽  
Author(s):  
M. Molnár-Láng ◽  
G. Galiba ◽  
G. Kovács ◽  
J. Sutka
Keyword(s):  
Triticum Aestivum ◽  
Hordeum Vulgare ◽  
Seed Set ◽  
Intergeneric Hybrid ◽  
Triticum Aestivum L ◽  
Meiotic Behaviour ◽  
Tissue Cultures ◽  
Hordeum Vulgare L ◽  
Regenerant Plants

A total of 41 regenerant plants were raised from two consecutive in vitro propagation cycles from a barley (Hordeum vulgare L. cv. Martonvásári 50) × wheat (Triticum aestivum L. cv. Chinese Spring) hybrid. The second-cycle regenerants exhibited great variability for most morphological traits. The female fertility of certain regenerant hybrids considerably exceeded that of the initial hybrid, giving substantially higher seed set when pollinated with wheat. This character was transferred to the BC1 and BC2 progeny produced from the regenerants. The cytological analysis of the second-cycle regenerants indicated that these had a higher degree of meiotic instability than the initial hybrid. The proportion of cells with 28 chromosomes (21 wheat + 7 barley) dropped to one-half of that in the initial hybrid, with a rise in the number of hypoploid and hyperploid cells. The number of chiasmata per cell increased from 1.7 in the initial hybrid to 4.7 in the regenerants, and there was also an increase in the number of misdivisions.Key words: intergeneric hybrid, tissue culture, fertility, meiotic behaviour, somaclonal variation.

An investigation on in vitro propagation of Colchicum luteum and its evaluation of colchicine for the economic benefit of rural people in West Bengal

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
M Gangopadhyay ◽  
R Bhattacharya ◽  
D Chakraborty ◽  
S Bhattacharya ◽  
A Mitra ◽  
...  
Keyword(s):  
Economic Benefit ◽  
In Vitro Propagation ◽  
West Bengal ◽  
Rural People

In vitro propagation of Troyer Citrange : A horticulturally important citrus rootstock

Green Farming ◽  
2020 ◽  
Vol 11 (2-3) ◽  
pp. 130
Author(s):  
VIJAYAKUMARI N. ◽  
P. GHOSH ◽  
Y.B. LAHANE ◽  
K.P. FISKE
Keyword(s):  
In Vitro Propagation ◽  
Citrus Rootstock ◽  
Troyer Citrange

scholarly journals In vitro propagation of some Cyrtanthus species

1998 ◽  
Vol 64 (3) ◽  
pp. 228-231 ◽  
Author(s):  
B.G. McAlister ◽  
A. Strydom ◽  
J. van Staden
Keyword(s):  
In Vitro Propagation

scholarly journals In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic Huperzia serrata

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1112
Author(s):  
Yan Yang ◽  
Liangfang Dai ◽  
Decai Wu ◽  
Limin Dong ◽  
Yisheng Tu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
In Vitro Propagation ◽  
Huperzine A ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Regeneration Rate ◽  
Huperzia Serrata ◽  
Chromatography Analysis

Huperzia serrata is a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation of H. serrata is considered a new way to relieve the resource pressure of H. serrata. In this study, three different genotypic wild H. serrata were used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regenerated H. serrata were investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium for H. serrata was the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L−1 Naphthalene acetic acid (NAA) and 0.1 mg·L−1 2,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L−1), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90–87.17 µg·g−1. The antioxidant experiment showed that the methanol extract of in vitro H. serrata had higher antioxidant activity than that of wild H. serrata. This study provides a reliable in vitro H. serrata culture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA.

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