High frequency, heat treatment-induced inactivation of the phosphinothricin resistance gene in transgenic single cell suspension cultures of Medicago sativa

1992 ◽  
Vol 235 (2-3) ◽  
pp. 189-196 ◽  
Author(s):  
Christian Walter ◽  
Inge Broer ◽  
Doris Hillemann ◽  
Alfred Pühler
Keyword(s):  
Heat Treatment ◽  
Medicago Sativa ◽  
Cell Suspension ◽  
Single Cell ◽  
Resistance Gene ◽  
High Frequency ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Single Cell Suspension

Abscisic acid and low temperature induced polypeptide changes in alfalfa (Medicago sativa) cell suspension cultures

1986 ◽  
Vol 64 (11) ◽  
pp. 2758-2763 ◽  
Author(s):  
Albert J. Robertson ◽  
Lawrence V. Gusta
Keyword(s):  
Abscisic Acid ◽  
Low Temperature ◽  
Medicago Sativa ◽  
Cell Suspension ◽  
Sodium Dodecyl ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Total Cell ◽  
Cell Protein ◽  
Major Protein

Changes in extracellular, cellular, and subcellular proteins during abscisic acid and low temperature induced cold hardening of alfalfa (Medicago sativa L. cv. Wisconsin 22C) cell suspension cultures were investigated by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Extracellular proteins from 4- to 6-day-old ABA and low temperature grown alfalfa cells showed decreased electrophoretic mobilities, lacked a 190-kDa glycoprotein, and had reduced amounts of four other polypeptides. In total cell protein analyses, a 42-kDa protein was enriched in both ABA and low temperature treated alfalfa cells. Several proteins increased or induced by exogenous ABA treatment were identified in the extracellular (12.5 and 13 to 15 kDa), total cell and cell wall (24 kDa), and soluble (20, 37, and 41 kDa) fractions. However, no major protein changes were resolved by one-dimensional electrophoretic analyses of crude membrane proteins.

High-frequency plant regeneration from embryogenic cell suspension cultures of Gynura procumbens

2018 ◽  
Vol 13 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Eun Yee Jie ◽  
Nornita Sham Atong ◽  
Woo Seok Ahn ◽  
Myung Suk Ahn ◽  
Byung Hwan Min ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
High Frequency ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Embryogenic Cell Suspension ◽  
Embryogenic Cell
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