Acid alcoholic brilliant cresyl blue stain for gastric and other mucins

1969 ◽  
Vol 17 (2) ◽  
pp. 138-144 ◽  
Author(s):  
R. D. Lillie ◽  
P. Pizzolato
Keyword(s):  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Blue Stain

Potential pitfalls of comparative measurements of reticulocytes with flow cytometry and microscopy in prematures and infants

2004 ◽  
Vol 42 (10) ◽  
Author(s):  
Gesa Wiegand ◽  
Anja Effenberger-Klein ◽  
Rainer Weber ◽  
Wolfgang Kosanke ◽  
Dietrich Niethammer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Blood Cells ◽  
Age Groups ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Blue Stain ◽  
Good Concordance ◽  
Advia 120 ◽  
Microscopic Counting ◽  
Good Agreement

AbstractReticulocyte counting by flow cytometry (Bayer H*3, ADVIA 120) in blood of prematures, infants and children > 1 year of age was compared with microscopic counting under research conditions (9000 counted red blood cells per slide). While in children > 1 year a good concordance of both methods was observed, 2.3–2.4-fold higher values were obtained in neonates by microscopy (Brilliant Cresyl Blue stain, 0.5%). However, another laboratory found good agreement between H*3-counting and microscopy in samples also obtained from neonates using the same methods. Despite very similar results for all age groups in comparative flow cytometry measurements in both laboratories, counting of smears from neonates differed, showing an approximately 2.3-fold larger amount of reticulocytes in our laboratory. The reason for these observations was a greater enlargement (1250-fold) used routinely in our laboratory compared with 800-fold in the other one. Thus very mature reticulocytes frequently found in neonates could only be detected using a 1250-fold enlargement. Similarly, the low concentration of the colouring matter used in the H*3 (0.0005% oxazin or 0.001% ADVIA 120) is obviously not sufficient for detection of mature reticulocytes. Therefore, it is important to consider this phenomenon and to standardise microscopic enlargement, especially for comparisons in multicentre studies.

scholarly journals Impact of Using Brilliant Cresyl Blue Stain on Oocyte and Embryo Selection: Review Article

2017 ◽  
Vol 48 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Karima Ghoneimy M. ◽  
Al-shimaa El-Naby ◽  
mohamed Elraay ◽  
Youssef Youssef ◽  
gamal sosa ◽  
...  
Keyword(s):  
Review Article ◽  
Embryo Selection ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Blue Stain

scholarly journals Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

2003 ◽  
Vol 43 (2) ◽  
pp. 179-187 ◽  
Author(s):  
Elisabeth Rodríguez-González ◽  
Manel López-Bejar ◽  
Dolors Izquierdo ◽  
María-Teresa Paramio
Keyword(s):  
Developmental Competence ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue

scholarly journals Effect of adding growth factors during in vitro maturation on the developmental potentials of ewe oocytes selected by brilliant cresyl blue staining

2021 ◽  
Vol 14 (2) ◽  
pp. 452-456
Author(s):  
Mohamed Fathi ◽  
Amr F. Elkarmoty
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Embryo Development ◽  
In Vitro Maturation ◽  
Blue Staining ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Nuclear Maturation ◽  
Maturation Medium

Aim: Several factors had been concerned with the developmental competence of the sheep oocyte. This study aims to investigate the effect of adding growth factors (insulin-like growth factor 1 [IGF-1] and epidermal growth factor [EGF]) in the maturation medium of ewe oocytes selected based on brilliant cresyl blue (BCB) screening on in vitro maturation (IVM), fertilization, and pre-implantation embryo development. Materials and Methods: Cumulus-oocyte complexes (COCs) were obtained from the ovaries of slaughtered ewes by either aspiration or slicing techniques. COCs were in vitro matured in a medium containing IGF-1 and EGF (control group). For BCB screening, oocytes were stained and divided into BCB+ oocytes that matured in the same maturation conditions without adding growth factors (Group 2) or in the presence of growth factors (Group 3), and BCB– oocytes that matured in medium without growth factors (Group 4) or with growth factors (Group 5). Results: The supplementation of the maturation medium with growth factors during IVM of (BCB+) oocytes resulted in a significant increase in nuclear maturation rate (90.9%), fertilization rate (75.6%), and embryo developmental rates (60.0%, 46.7%, and 33.3% for cleavage, morula, and blastocyst, respectively). Conclusion: Culturing BCB+ oocytes in a maturation medium containing both EGF and IGF-1 showed a significant improvement in nuclear maturation, fertilization, and pre-implantation embryo development in vitro.

Combination of oocyte and zygote selection by brilliant cresyl blue (BCB) test enhanced prediction of developmental potential to the blastocyst in cattle

2013 ◽  
Vol 136 (4) ◽  
pp. 245-251 ◽  
Author(s):  
S.M. Mirshamsi ◽  
H. KaramiShabankareh ◽  
M. Ahmadi-Hamedani ◽  
L. Soltani ◽  
H. Hajarian ◽  
...  
Keyword(s):  
Developmental Potential ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue

scholarly journals Brilliant Cresyl Blue as a Stain for Plant Chromosomes

Nature ◽  
1965 ◽  
Vol 207 (4994) ◽  
pp. 329-329 ◽  
Author(s):  
B. C. ARNOLD
Keyword(s):  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Plant Chromosomes

Electrocatalytic oxidation of NADH by Brilliant Cresyl Blue–DNA intercalation adduct

2005 ◽  
Vol 50 (5) ◽  
pp. 1107-1112 ◽  
Author(s):  
Patricia de-los-Santos-Álvarez ◽  
M. Jesús Lobo-Castañón ◽  
Arturo J. Miranda-Ordieres ◽  
Paulino Tuñón-Blanco
Keyword(s):  
Electrocatalytic Oxidation ◽  
Dna Intercalation ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue
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