Linear Sweep Voltammetric Studies on the Interaction of Brilliant Cresyl Blue With Nucleic Acids and its Analytical Application

2006 ◽  
Vol 96 (5-6) ◽  
pp. 259-271 ◽  
Author(s):  
Wei Sun ◽  
Jiayu You ◽  
Chaokun Gong ◽  
Kui Jiao
Keyword(s):  
Nucleic Acids ◽  
Analytical Application ◽  
Linear Sweep ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Voltammetric Studies

Brilliant cresyl blue as a new red region fluorescent probe for determination of nucleic acids

2000 ◽  
Vol 64 (3) ◽  
pp. 263-269 ◽  
Author(s):  
Hong Zheng ◽  
Xiao-Lan Chen ◽  
Chang-Qing Zhu ◽  
Dong-Hui Li ◽  
Qiu-Ying Chen ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fluorescent Probe ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue

scholarly journals Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

2003 ◽  
Vol 43 (2) ◽  
pp. 179-187 ◽  
Author(s):  
Elisabeth Rodríguez-González ◽  
Manel López-Bejar ◽  
Dolors Izquierdo ◽  
María-Teresa Paramio
Keyword(s):  
Developmental Competence ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue

scholarly journals Effect of adding growth factors during in vitro maturation on the developmental potentials of ewe oocytes selected by brilliant cresyl blue staining

2021 ◽  
Vol 14 (2) ◽  
pp. 452-456
Author(s):  
Mohamed Fathi ◽  
Amr F. Elkarmoty
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Embryo Development ◽  
In Vitro Maturation ◽  
Blue Staining ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Nuclear Maturation ◽  
Maturation Medium

Aim: Several factors had been concerned with the developmental competence of the sheep oocyte. This study aims to investigate the effect of adding growth factors (insulin-like growth factor 1 [IGF-1] and epidermal growth factor [EGF]) in the maturation medium of ewe oocytes selected based on brilliant cresyl blue (BCB) screening on in vitro maturation (IVM), fertilization, and pre-implantation embryo development. Materials and Methods: Cumulus-oocyte complexes (COCs) were obtained from the ovaries of slaughtered ewes by either aspiration or slicing techniques. COCs were in vitro matured in a medium containing IGF-1 and EGF (control group). For BCB screening, oocytes were stained and divided into BCB+ oocytes that matured in the same maturation conditions without adding growth factors (Group 2) or in the presence of growth factors (Group 3), and BCB– oocytes that matured in medium without growth factors (Group 4) or with growth factors (Group 5). Results: The supplementation of the maturation medium with growth factors during IVM of (BCB+) oocytes resulted in a significant increase in nuclear maturation rate (90.9%), fertilization rate (75.6%), and embryo developmental rates (60.0%, 46.7%, and 33.3% for cleavage, morula, and blastocyst, respectively). Conclusion: Culturing BCB+ oocytes in a maturation medium containing both EGF and IGF-1 showed a significant improvement in nuclear maturation, fertilization, and pre-implantation embryo development in vitro.

Combination of oocyte and zygote selection by brilliant cresyl blue (BCB) test enhanced prediction of developmental potential to the blastocyst in cattle

2013 ◽  
Vol 136 (4) ◽  
pp. 245-251 ◽  
Author(s):  
S.M. Mirshamsi ◽  
H. KaramiShabankareh ◽  
M. Ahmadi-Hamedani ◽  
L. Soltani ◽  
H. Hajarian ◽  
...  
Keyword(s):  
Developmental Potential ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue

scholarly journals Brilliant Cresyl Blue as a Stain for Plant Chromosomes

Nature ◽  
1965 ◽  
Vol 207 (4994) ◽  
pp. 329-329 ◽  
Author(s):  
B. C. ARNOLD
Keyword(s):  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Plant Chromosomes

Electrocatalytic oxidation of NADH by Brilliant Cresyl Blue–DNA intercalation adduct

2005 ◽  
Vol 50 (5) ◽  
pp. 1107-1112 ◽  
Author(s):  
Patricia de-los-Santos-Álvarez ◽  
M. Jesús Lobo-Castañón ◽  
Arturo J. Miranda-Ordieres ◽  
Paulino Tuñón-Blanco
Keyword(s):  
Electrocatalytic Oxidation ◽  
Dna Intercalation ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue

Acid alcoholic brilliant cresyl blue stain for gastric and other mucins

1969 ◽  
Vol 17 (2) ◽  
pp. 138-144 ◽  
Author(s):  
R. D. Lillie ◽  
P. Pizzolato
Keyword(s):  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Blue Stain

195 RELATIVE mRNA EXPRESSION OF 4 CANDIDATES IN LAMB OOCYTES SELECTED BY BRILLIANT CRESYL BLUE STAINING

2013 ◽  
Vol 25 (1) ◽  
pp. 246
Author(s):  
M. G. Catalá ◽  
M. Roura ◽  
D. Izquierdo ◽  
S. Hammammi ◽  
S. Uzbekova ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Relative Concentration ◽  
Rna Extraction ◽  
Melting Curve ◽  
Elongation Factor ◽  
Oocyte Quality ◽  
Blue Staining ◽  
Melting Curve Analysis ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue

Brilliant cresyl blue (BCB) staining determines the activity of glucose-6-phosphate dehydrogenase (G6PDH), an enzyme in which activity decreases as the oocytes reach their growth phase. We have previously shown in lamb the effectiveness of this stain in selecting the largest and most competent oocytes to develop up to the blastocyst stage (Catala et al. 2011 Reproduction 142, 517–527). The aim of this study was to analyse the expression of 4 genes related to oocyte quality in BCB-selected oocytes. The 4 genes analyzed in this study were selected after a literature review in which they were associated with a better oocyte quality or embryo development; 2 of these genes were related to cell cycle: nuclear auto-antigenic sperm protein (NASP) and histone H2A (H2A.Z), and 2 genes with enzymatic functions: peroxiredoxin (PRDX1) and elongation factor-A1 (EEF1A1). Oocytes were recovered after slicing the surface of lamb ovaries (3 to 5 months old) obtained from a local slaughterhouse. Oocytes with more than 3 compact cumulus layers and homogenic cytoplasm were selected and exposed to 13 µM BCB during 1 h before IVM and classified according to oocyte coloration: oocytes with blue cytoplasm or grown oocytes (BCB+) and oocytes not coloured or growing oocytes (BCB–). Cumulus–oocyte complexes were then matured in conventional TCM199 medium supplemented with 10% fetal bovine serum and hormones during 24 h in a controlled atmosphere. Groups of 15 denuded oocytes (3 replicates) were taken before (0 h) and after maturation (24 h) and stored at –80°C in 100 mL of Trizol until use. After RNA extraction following manufacturer’s protocol (Promega, Madison, WI, USA), reverse transcription was performed by extended cDNA using Oligo (dT) primers during 5 min at 70°C and 1 h at 65°C using superscript III (200 U mM–1; Invitrogen). Relative qualitative PCR analyses were performed in duplicate using SYBR Green Fluorophore kit (Bio-Rad, Hercules, CA, USA). The specificity of each PCR product was determined by a melting curve analysis and the amplicon size determination by an agarose gel. A standard curve was also included, consisting of corresponding plasmid DNA fragments from 1 pg to 0.1 fg, purified with QIAquick PCR Purification Kit (Qiagen, Valencia, CA, USA). Correlation coefficients and PCR efficiencies were considered between 85 and 100%. Finally, the results for mRNA were normalized according to the relative concentration of the internal and external gene (luciferase and 18S, respectively). The statistical analysis was performed by One-way ANOVA in GraphPad Prism v 3 (GraphPad Software, San Diego, CA, USA). The analysis of gene expression showed no differences in relation to BCB classification. The only difference we found is a significant decrease (P ≤ 0.05) in the RE of PRDX observed in matured oocytes compared with immature ones. In conclusion, the expression of the mRNA expression of NASP, H2A.Z, PRDX1, and EEF1A1 were not affected by oocyte quality.

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