A simple and safe technique for sterile autologous platelet labelling using ?Monovette? vials

1984 ◽  
Vol 9 (7) ◽  
Author(s):  
H. Sinzinger ◽  
H. Kolbe ◽  
Eva Strobl-J�ger ◽  
R. H�fer
Keyword(s):  
Platelet Labelling ◽  
Safe Technique ◽  
Autologous Platelet

MICROEMBOLISATION DURING SURGICAL SHOCK: EFFECT OF PROSTAGLANDIN E1

1987 ◽  
Author(s):  
K R Poskitt ◽  
J T C Irwin ◽  
C M Backhouse ◽  
C N McCollum
Keyword(s):  
Prostaglandin E1 ◽  
Major Surgery ◽  
Midline Laparotomy ◽  
Venous Shunt ◽  
Platelet Labelling ◽  
Platelet Aggregate ◽  
To Receive ◽  
Surgical Shock ◽  
Autologous Platelet ◽  
Postoperative Pulmonary Dysfunction

Embolisation of microaggregates following major surgery may be a cause of pulmonary arterio-venous shunt and postoperative respiratory failure (1). Prostaglandin E1 may prevent intravascular aggregation and we studied this possibility in a pig model of surgical shock.Following autologous platelet labelling with Indium, 16 pigs (20-30kg) were randomised to receive a perioperative infusion of PGE1 (100ng/kg/min) or placebo. Arterial and Swann Ganz catheters were inserted under anaesthesia prior to surgery consisting of midline laparotomy, exteriorisation of small bowel 1.5 hours of aortic clamping and 1 hour of hypotension. On induction, during shock and at 3 days in survivors platelet and leucocyte count, blood radioactivity, venous aggregates (SFP), lung platelet uptake (LPU), pulmonary vascular resistance (PVR) and alveolar-arterial p02 difference (A-ad02) were measured.All results mean ± sem *p <0.05 Mann Whitney U-testDuring surgical shock, the formation of venous aggregates, the fall in circulating radiolabelled platelets and their accumulation in lungs were reduced by PGE1 (p< 0.05). BP, CVP and PWP were all lower on PGE1 and at 3 days the improvement in A-ad02 in PGE1 pigs failed to reach significance.PGE1 reduced platelet aggregate formation and their subsequent pulmonary microembolisation despite worsening shock due to vasodilation.1. McCollum CN, Campbell IT. The value of measuring intravascular platelet aggregation in the prediction of postoperative pulmonary dysfunction. Br J Surg 1979: 66; 703-707

Autologous platelet labelling using “Monovette” vials is not septic

1986 ◽  
Vol 12 (9) ◽  
pp. 471-471
Author(s):  
Helmut Sinzinger ◽  
Eva Strobl-Jäger ◽  
Rudolf Höfer
Keyword(s):  
Platelet Labelling ◽  
Autologous Platelet

PULMONARY MICROEMBOLISATION IN SURGICAL SHOCK: THE EFFECT OF CYCLO-OXYGENASE INHIBITION

1987 ◽  
Author(s):  
C M Backhouse ◽  
A C Meek ◽  
K R Poskitt ◽  
C N McCollum
Keyword(s):  
Pulmonary Function ◽  
Aortic Surgery ◽  
High Dose ◽  
Midline Laparotomy ◽  
Arteriovenous Shunts ◽  
Pulmonary Shunting ◽  
Platelet Labelling ◽  
Arterial Po2 ◽  
Surgical Shock ◽  
Autologous Platelet

Thromboxane release from platelet microemboli during major arterial surgery may mediate depression of cardio-pulmonary function. The effect of cyclo-oxygenase inhibition by aspirin has been studied in a porcine model of aortic surgery.Following autologous platelet labelling with 111-lndium, 24 pigs (20-25kg) were randomised to low dose (LD) aspirin (0.5mg/kg), high dose (HD) aspirin (10mg/kg) or placebo.Arterial and Swann Ganz catheters were inserted prior to surgery consisting of midline laparotomy, small bowel extériorisation, 1.5 hours of aortic clamping and 1 hour shock before resuscitation. On induction, during shock and at 3 days, platelet and leucocyte counts, lung platelet uptake (LPU), venous aggregates by screen filtration (SFP), mean arterial pressure (BP), cardiac output (CO), pulmonary shunt (PS) and alveolar-arterial pO2 difference (A-adO2) were measured.During shock following aortic declamping aspirin preserved blood pressure by increasing vascular resistance rather than CO. Venous aggregates by SFP tended to be lower on aspirin with significantly reduced LPU, subsequent pulmonary shunting and A-adO2. The improvement in PS but not A-adO2 remained significant at 3 days (p<0.05).Cyclo-oxygenase inhibition improved pulmonary function during surgical shock either by inhibiting platelet microemboli or by a direct effect on pulmonary arteriovenous shunts.

Platelet Deposition on Human Atherosclerotic Lesions is Decreased by Low-Dose Aspirin in Combination with Dipyridamole

1988 ◽  
Vol 16 (1) ◽  
pp. 39-43 ◽  
Author(s):  
H. Sinzinger ◽  
J. O'Grady ◽  
P. Fitscha
Keyword(s):  
Half Life ◽  
Low Dose ◽  
Combined Therapy ◽  
Peripheral Vascular ◽  
Atherosclerotic Lesions ◽  
Dose Aspirin ◽  
Low Dose Aspirin ◽  
Platelet Labelling ◽  
Before And After ◽  
Autologous Platelet

Eighteen patients with ischaemic peripheral vascular disease were treated for a 5-week period with either 20 mg aspirin daily, 75 mg dipyridamole three times daily or a combination of these two treatments. Before and after 4 weeks' treatment autologous platelet labelling with 111In was carried out and sites of active vascular platelet uptake monitored, and platelet half-life measured. Neither aspirin nor dipyridamole alone had any effect on platelet uptake or on platelet half-life. The combination of aspirin and dipyridamole resulted in a significant decrease in platelet uptake and a nonsignificant trend towards prolongation of platelet half-life. These findings suggest that this combined therapy may be of benefit in the treatment of atherosclerosis in man.

Pronation Technique in ARDS Patients

ABOUTOPEN ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 21-23
Author(s):  
Raffaele Di Fenza ◽  
Hedwige Gay ◽  
Martina Favarato ◽  
Isabella Fontana ◽  
Roberto Fumagalli
Keyword(s):  
Intensive Care ◽  
Intensive Care Units ◽  
Distress Syndrome ◽  
Daily Practice ◽  
Volume Distribution ◽  
Intensive Care Unit Staff ◽  
Safe Technique ◽  
Fraction Of Inspired Oxygen ◽  
And Diffusion ◽  
Inspired Oxygen

In severe acute respiratory distress syndrome (ARDS), characterized by the ratio of arterial partial pressure of oxygen over fraction of inspired oxygen (P/F) less than 150 mm Hg, pronation cycles are the only intervention that showed improved survival, in combination with protective ventilation. The physiological advantages of performing pronation cycles, such as the improvement of oxygenation, better tidal volume distribution with increased involvement of dorsal regions, and easier drainage of secretions, overcome the possible complications, that is, endotracheal tube occlusion or misplacement, pressure ulcers, and brachial plexus injury. However, the incidence of complications is dramatically lower in intensive care units with expertise, adopting prone positioning in daily practice. In this video we are proposing step by step an easy and ergonomic technique to perform pronation maneuvers in patients with severe ARDS. Recent literature suggests that a high percentage of these patients are treated without undergoing pronation cycles. The main purpose of this video is to help increase the number of intensive care units worldwide commonly performing pronation cycles in patients that have indications to be pronated, in order to decrease healthcare burden and costs directly caused by ARDS. Proper intensive care unit staff training is fundamental in minimizing the risks associated with the maneuver for both patients and operators; and diffusion of a safe technique encouraging the operators is the second main purpose of this video.

Autologous platelet-rich plasma for assisted reproduction

2021 ◽  
Author(s):  
Dennis Vaidakis ◽  
Eleni Sertedaki ◽  
Vasilios Karageorgiou ◽  
Charalampos S Siristatidis
Keyword(s):  
Assisted Reproduction ◽  
Platelet Rich Plasma ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet
Sign in / Sign up

Export Citation Format

Share Document