Autologous platelet labelling using “Monovette” vials is not septic

1986 ◽  
Vol 12 (9) ◽  
pp. 471-471
Author(s):  
Helmut Sinzinger ◽  
Eva Strobl-Jäger ◽  
Rudolf Höfer
Keyword(s):  
Platelet Labelling ◽  
Autologous Platelet

MICROEMBOLISATION DURING SURGICAL SHOCK: EFFECT OF PROSTAGLANDIN E1

1987 ◽  
Author(s):  
K R Poskitt ◽  
J T C Irwin ◽  
C M Backhouse ◽  
C N McCollum
Keyword(s):  
Prostaglandin E1 ◽  
Major Surgery ◽  
Midline Laparotomy ◽  
Venous Shunt ◽  
Platelet Labelling ◽  
Platelet Aggregate ◽  
To Receive ◽  
Surgical Shock ◽  
Autologous Platelet ◽  
Postoperative Pulmonary Dysfunction

Embolisation of microaggregates following major surgery may be a cause of pulmonary arterio-venous shunt and postoperative respiratory failure (1). Prostaglandin E1 may prevent intravascular aggregation and we studied this possibility in a pig model of surgical shock.Following autologous platelet labelling with Indium, 16 pigs (20-30kg) were randomised to receive a perioperative infusion of PGE1 (100ng/kg/min) or placebo. Arterial and Swann Ganz catheters were inserted under anaesthesia prior to surgery consisting of midline laparotomy, exteriorisation of small bowel 1.5 hours of aortic clamping and 1 hour of hypotension. On induction, during shock and at 3 days in survivors platelet and leucocyte count, blood radioactivity, venous aggregates (SFP), lung platelet uptake (LPU), pulmonary vascular resistance (PVR) and alveolar-arterial p02 difference (A-ad02) were measured.All results mean ± sem *p <0.05 Mann Whitney U-testDuring surgical shock, the formation of venous aggregates, the fall in circulating radiolabelled platelets and their accumulation in lungs were reduced by PGE1 (p< 0.05). BP, CVP and PWP were all lower on PGE1 and at 3 days the improvement in A-ad02 in PGE1 pigs failed to reach significance.PGE1 reduced platelet aggregate formation and their subsequent pulmonary microembolisation despite worsening shock due to vasodilation.1. McCollum CN, Campbell IT. The value of measuring intravascular platelet aggregation in the prediction of postoperative pulmonary dysfunction. Br J Surg 1979: 66; 703-707

PULMONARY MICROEMBOLISATION IN SURGICAL SHOCK: THE EFFECT OF CYCLO-OXYGENASE INHIBITION

1987 ◽  
Author(s):  
C M Backhouse ◽  
A C Meek ◽  
K R Poskitt ◽  
C N McCollum
Keyword(s):  
Pulmonary Function ◽  
Aortic Surgery ◽  
High Dose ◽  
Midline Laparotomy ◽  
Arteriovenous Shunts ◽  
Pulmonary Shunting ◽  
Platelet Labelling ◽  
Arterial Po2 ◽  
Surgical Shock ◽  
Autologous Platelet

Thromboxane release from platelet microemboli during major arterial surgery may mediate depression of cardio-pulmonary function. The effect of cyclo-oxygenase inhibition by aspirin has been studied in a porcine model of aortic surgery.Following autologous platelet labelling with 111-lndium, 24 pigs (20-25kg) were randomised to low dose (LD) aspirin (0.5mg/kg), high dose (HD) aspirin (10mg/kg) or placebo.Arterial and Swann Ganz catheters were inserted prior to surgery consisting of midline laparotomy, small bowel extériorisation, 1.5 hours of aortic clamping and 1 hour shock before resuscitation. On induction, during shock and at 3 days, platelet and leucocyte counts, lung platelet uptake (LPU), venous aggregates by screen filtration (SFP), mean arterial pressure (BP), cardiac output (CO), pulmonary shunt (PS) and alveolar-arterial pO2 difference (A-adO2) were measured.During shock following aortic declamping aspirin preserved blood pressure by increasing vascular resistance rather than CO. Venous aggregates by SFP tended to be lower on aspirin with significantly reduced LPU, subsequent pulmonary shunting and A-adO2. The improvement in PS but not A-adO2 remained significant at 3 days (p<0.05).Cyclo-oxygenase inhibition improved pulmonary function during surgical shock either by inhibiting platelet microemboli or by a direct effect on pulmonary arteriovenous shunts.

Platelet Deposition on Human Atherosclerotic Lesions is Decreased by Low-Dose Aspirin in Combination with Dipyridamole

1988 ◽  
Vol 16 (1) ◽  
pp. 39-43 ◽  
Author(s):  
H. Sinzinger ◽  
J. O'Grady ◽  
P. Fitscha
Keyword(s):  
Half Life ◽  
Low Dose ◽  
Combined Therapy ◽  
Peripheral Vascular ◽  
Atherosclerotic Lesions ◽  
Dose Aspirin ◽  
Low Dose Aspirin ◽  
Platelet Labelling ◽  
Before And After ◽  
Autologous Platelet

Eighteen patients with ischaemic peripheral vascular disease were treated for a 5-week period with either 20 mg aspirin daily, 75 mg dipyridamole three times daily or a combination of these two treatments. Before and after 4 weeks' treatment autologous platelet labelling with 111In was carried out and sites of active vascular platelet uptake monitored, and platelet half-life measured. Neither aspirin nor dipyridamole alone had any effect on platelet uptake or on platelet half-life. The combination of aspirin and dipyridamole resulted in a significant decrease in platelet uptake and a nonsignificant trend towards prolongation of platelet half-life. These findings suggest that this combined therapy may be of benefit in the treatment of atherosclerosis in man.

Autologous platelet-rich plasma for assisted reproduction

2021 ◽  
Author(s):  
Dennis Vaidakis ◽  
Eleni Sertedaki ◽  
Vasilios Karageorgiou ◽  
Charalampos S Siristatidis
Keyword(s):  
Assisted Reproduction ◽  
Platelet Rich Plasma ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

scholarly journals Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation—A Narrative Review

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 317
Author(s):  
Eitan Mijiritsky ◽  
Haya Drora Assaf ◽  
Oren Peleg ◽  
Maayan Shacham ◽  
Loredana Cerroni ◽  
...  
Keyword(s):  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Periodontal Regeneration ◽  
Clinical Work ◽  
Gingival Recession ◽  
Vital Role ◽  
Facial Rejuvenation ◽  
Cochrane Library ◽  
The Past ◽  
Autologous Platelet

Growth factors (GFs) play a vital role in cell proliferation, migration, differentiation and angiogenesis. Autologous platelet concentrates (APCs) which contain high levels of GFs make them especially suitable for periodontal regeneration and facial rejuvenation. The main generations of APCs presented are platelet-rich plasma (PRP), platelet-rich fibrin (PRF) and concentrated growth factor (CGF) techniques. The purpose of this review is to provide the clinician with an overview of APCs’ evolution over the past decade in order to give reliable and useful information to be used in clinical work. This review summarizes the most interesting and novel articles published between 1997 and 2020. Electronic and manual searches were conducted in the following databases: Pubmed, Scopus, Cochrane Library and Embase. The following keywords were used: growth factors, VEGF, TGF-b1, PRP, PRF, CGF and periodontal regeneration and/or facial rejuvenation. A total of 73 articles were finally included. The review then addresses the uses of the three different techniques in the two disciplines, as well as the advantages and limitations of each technique. Overall, PRP is mainly used in cases of hard and soft tissue procedures, while PRF is used in gingival recession and the treatment of furcation and intrabony defects; CGF is mainly used in bone regeneration.

Sign in / Sign up

Export Citation Format

Share Document