Efficient direct chromosome analyses and enzyme determinations from chorionic villi samples in the first trimester of pregnancy

1983 ◽  
Vol 63 (4) ◽  
pp. 349-357 ◽  
Author(s):  
G. Simoni ◽  
B. Brambati ◽  
C. Danesino ◽  
F. Rossella ◽  
G. L. Terzoli ◽  
...  
Keyword(s):  
First Trimester ◽  
Chorionic Villi ◽  
First Trimester Of Pregnancy

scholarly journals Method of sampling chorionic villi in first trimester of pregnancy under guidance of real time ultrasound.

BMJ ◽  
1983 ◽  
Vol 286 (6377) ◽  
pp. 1542-1544 ◽  
Author(s):  
R H Ward ◽  
B Modell ◽  
M Petrou ◽  
F Karagozlu ◽  
E Douratsos
Keyword(s):  
Real Time ◽  
First Trimester ◽  
Chorionic Villi ◽  
First Trimester Of Pregnancy

scholarly journals INVESTIGATION OF TROPHOBLAST IN TORCH INFECTION ON ABORTION MATERIAL IN THE FIRST TRIMESTER OF PREGNANCY

2019 ◽  
Vol 18 (4) ◽  
pp. 58-62
Author(s):  
A. V. Goshovskaya ◽  
V. M. Goshovsky ◽  
S. M. Yasnikovska
Keyword(s):  
Gestational Age ◽  
Imaging System ◽  
Chorionic Villus ◽  
First Trimester ◽  
Control Group ◽  
Chorionic Villi ◽  
Infectious Process ◽  
First Trimester Of Pregnancy ◽  
Torch Infection ◽  
Immunohistochemical Studies

This study is a fragment of a series of immunohistochemical studies of trophoblast with TORCH infection, which are scheduled to be carried out at different gestational dates. This article deals with the results of trophoblast studies at the gestational age of 7-8 weeks. The study examined abortion material 7-8 weeks of gestation. The main group of the study consisted of 18 observations of TORCH infection, and the control group consisted of 17 observations of an aborted pregnancy without signs of an infectious process (abortion for social reasons). An immunohistochemical procedure was performed on metalloproteinases-2 with primary antibodies and polymer antigen imaging system using DAKO diaminobenzidine. The method of computer microdensitometry in a specialized computer program ImageJ evaluated the optical density of the color. According to the results of immunohistochemical studies using computer microdensitometry at the gestational age of 7-8 weeks, both with TORCH infection and without it an infectious process, strong expression of metalloproteinase-2 is observed in the invasive trophoblast, the smallest – in the syncytotrophoblast of the chorionic villi, and intermediate values are noted in chorionic villus cytotrophoblast and cell column cytotrophoblast. With TORCH infection the expression of metalloproteinase-2 decreases in all the four types of trophoblast (cytotrophoblast of chorionic villi; cytotrophoblast of cell columns; invasive cytotrophoblast in endometrial fragments), except for syncytotrophoblast of chorionic villi was found.

scholarly journals Uterine Glands Provide Histiotrophic Nutrition for the Human Fetus during the First Trimester of Pregnancy

2002 ◽  
Vol 87 (6) ◽  
pp. 2954-2959 ◽  
Author(s):  
Graham J. Burton ◽  
Adrian L. Watson ◽  
Joanne Hempstock ◽  
Jeremy N. Skepper ◽  
Eric Jauniaux
Keyword(s):  
First Trimester ◽  
Maternal Blood ◽  
Yolk Sac ◽  
Chorionic Villi ◽  
Intervillous Space ◽  
Human Blastocyst ◽  
Fetal Nutrition ◽  
First Trimester Of Pregnancy ◽  
Uterine Glands ◽  
Phagocytic Uptake

Providing adequate nutrition to the fetus is key to a successful pregnancy. The interstitial form of implantation displayed by the human blastocyst is generally associated with early onset of maternal blood flow to the developing placenta, and hence hemotrophic exchange. However, the recent finding that the maternal intraplacental circulation is not fully established until the third month of gestation suggests that human fetal nutrition may be initially histiotrophic. We therefore investigated activity of the uterine glands during early pregnancy. We demonstrate here that these glands remain active until at least wk 10 of pregnancy, and that their secretions are delivered freely into the placental intervillous space. We also demonstrate phagocytic uptake by the placental syncytiotrophoblast of two glycoproteins, the mucin MUC-1 and glycodelin A, synthesized in the maternal glands. Glycodelin was also detected within the epithelium of the secondary yolk sac lining the exocoelomic cavity, indicating that the yolk sac may play an important role in nutrient exchange before vascularisation of the chorionic villi. Our findings demonstrate that the uterine glands are an important source of nutrients during organogenesis, when metabolism is essentially anaerobic.

scholarly journals Peculiarity of mitotic activity of chorionic villi cytotrofoblast on the first trimester of pregnancy

2012 ◽  
Vol 61 (3) ◽  
pp. 115-122
Author(s):  
Irina Leonidovna Trofimova ◽  
Eva Valer’evna Evdokimenko ◽  
Tatyana Vladimirovna Kuznetzova
Keyword(s):  
Mitotic Activity ◽  
Proliferative Activity ◽  
First Trimester ◽  
Pericentric Heterochromatin ◽  
Missed Abortion ◽  
Chorionic Villi ◽  
First Trimester Of Pregnancy

Mitotic activity of chorionic villi cytotrophoblast of the 1st trimester at normal and missed pregnancy was studied. Proliferative activity of cytotrophoblast from missed abortions was somewhat increased in vitro. Application of a semidirect method of chromosomal preparation from chorionic villi is more efficient and provides better resolution for cytogenetic diagnostics of missed abortion. Unusual peculiarity in organization and replication of pericentric heterochromatin regions of chromosomes 1, 9 and 16 was detected

scholarly journals Features of the histological structure of trophoblast and chorionic villi with recurrent pregnancy loss in women with thrombophilias

2017 ◽  
Vol 25 (4) ◽  
pp. 621-641
Author(s):  
A. I. Mirov ◽  
O. N. Kharkevich ◽  
O. E. Golofast ◽  
I. B. Glukhovets
Keyword(s):  
Significant Role ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Chorionic Villus ◽  
Fetal Loss ◽  
First Trimester ◽  
Control Group ◽  
Histological Structure ◽  
Chorionic Villi ◽  
First Trimester Of Pregnancy

The frequency of recurrent pregnancy loss does not tend to decrease. This pathology continues to be one of the important problems of modern medicine. It is known that thrombophilia can play a significant role in the etiology of spontaneous reproductive losses. However, the pathogenesis of recurrent spontaneous loss of pregnancy in the presence of maternal thrombophilia is not fully understood. Aim. To identify the features of the histological structure of trophoblasts and chorionic villi in the first trimester of pregnancy in women with thrombophilia and recurrent pregnancy loss, with careful exclusion of other possible causes of fetal loss syndrome. Material and Methods. Histological examination of 49 chorion tissue samples from 24 patients with thrombophilia and recurrent pregnancy loss in the first trimester (study group) was performed. The controls were samples of chorion tissue taken during artificial abortion in 33 healthy women who had a history of 2 or more spontaneous labor without significant complications. Thrombophilia diagnosis and hemostasis system state evaluation was performed for all patients on the basis of analysis of 30 parameters according to standard methods. All studies were conducted at the Regional clinical hospital № 8 in Ryazan as well as the scientific and clinical center of hematology, oncology and immunology of the Ryazan State Medical University named after academician I.P. Pavlov of Health Ministry of the Russian Federation. Statistical processing of the obtained results was carried out with the help of computer program package Statistica (version 10). Results. Significant differences in the histological structure of trophoblast and chorionic villi in the studied women were revealed, in comparison with those in the control group. It is proved that the presence of thrombophilia negatively affects the process of embryogenesis and contributes to a significant reduction in the area of the chorionic villus vessels in the first trimester of pregnancy. Conclusion. It is proved that the presence of thrombophilia has a negative effect on the process of embryogenesis and significantly reduces the vascular area of chorionic villi that can probably play a significant role in the pathogenesis of recurrent pregnancy loss.

scholarly journals Evidence for HPV DNA in the placenta of women who resorted to elective abortion

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Maria Teresa Bruno ◽  
Salvatore Caruso ◽  
Francesca Bica ◽  
Giulia Arcidiacono ◽  
Sara Boemi
Keyword(s):  
Preterm Birth ◽  
First Trimester ◽  
Hpv Infection ◽  
Cervical Canal ◽  
Chorionic Villi ◽  
Hpv Dna ◽  
Elective Abortion ◽  
Placental Villi ◽  
First Trimester Of Pregnancy ◽  
Papillomavirus Dna

Abstract Background It is believed that HPV infection can result in the death of placental trophoblasts and cause miscarriages or preterm birth. In clinical cases of placental villi positive for HPV DNA reported by other authors, contamination is suspected in the act of crossing the cervical canal. We analyzed placental samples of women who resorted to elective abortion obtained by hysterosuction of ovular material, bypassing any contact with the cervical canal and vagina. Methods We studied the chorionic villi of the placenta of 64 women who resorted to voluntary termination of pregnancy, in the first trimester. To avoid contamination of the villi by the cervical canal, we analyzed placental samples obtained by hysterosuction of ovular material, bypassing any contact with the cervical canal and vagina. All samples of chorionic villi were manually selected from the aborted material and subjected to research for HPV DNA. Results HPV DNA was detected in 10 out of 60 women (16.6%). The HPV DNA identified in the placenta belonged to genotypes 6, 16, 35, 53, and 90. Conclusion The study shows that papillomavirus DNA can infect the placenta and that placenta HPV infection can occur as early as the first trimester of pregnancy.

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