Laboratory evalution of a translocation double heterozygote for genetic control of Aedes aegypti

1978 ◽  
Vol 51 (4) ◽  
pp. 153-157
Author(s):  
D. K. Uppal ◽  
C. F. Curtis ◽  
V. K. Soni
Keyword(s):  
Aedes Aegypti ◽  
Genetic Control ◽  
Double Heterozygote

scholarly journals Short‐term suppression of Aedes aegypti using genetic control does not facilitate Aedes albopictus

2015 ◽  
Vol 72 (3) ◽  
pp. 618-628 ◽  
Author(s):  
Kevin Gorman ◽  
Josué Young ◽  
Lleysa Pineda ◽  
Ricardo Márquez ◽  
Nestor Sosa ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Genetic Control ◽  
Aedes Albopictus ◽  
Short Term

Long-term survival of introduced genes in a natural population of Aedes Aegypti (L.) (Diptera: Culicidae)

1981 ◽  
Vol 71 (1) ◽  
pp. 129-132 ◽  
Author(s):  
Nancy Lorimer
Keyword(s):  
Aedes Aegypti ◽  
Genetic Control ◽  
Genetic Markers ◽  
Natural Population ◽  
Control Experiment ◽  
Genetic Material ◽  
Entire Period ◽  
Term Survival ◽  
Insect Management

A population of Aedes aegypti (L.) near the Kenya coast was monitored for nearly a year after a genetic control experiment. Two genetic markers that were carried by released males but unknown to the region persisted in the population during the entire period of observation. The endurance of introduced genetic material in a natural population is an important step toward the use of genetic control in insect management.

scholarly journals Comparative field cage tests of the population suppressing efficiency of three genetic control systems for Aedes aegypti

Heredity ◽  
1976 ◽  
Vol 36 (1) ◽  
pp. 11-29 ◽  
Author(s):  
C F Curtis ◽  
K K Grover ◽  
S G Suguna ◽  
D K Uppal ◽  
K Dietz ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Control Systems ◽  
Genetic Control ◽  
Field Cage

scholarly journals Genetic control of Aedes aegypti: data-driven modelling to assess the effect of releasing different life stages and the potential for long-term suppression

2014 ◽  
Vol 7 (1) ◽  
pp. 68 ◽  
Author(s):  
Peter Winskill ◽  
Angela F Harris ◽  
Siân A Morgan ◽  
Jessica Stevenson ◽  
Norzahira Raduan ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Genetic Control ◽  
Data Driven ◽  
Life Stages

PARACENTRIC INVERSIONS AND DETECTION OF SEX LINKED RECESSIVE LETHALS IN AEDES AEGYPTI

1970 ◽  
Vol 12 (3) ◽  
pp. 635-650 ◽  
Author(s):  
Satish C. Bhalla
Keyword(s):  
Aedes Aegypti ◽  
Genetic Control ◽  
Chromosomal Aberrations ◽  
The Other ◽  
Crossing Over ◽  
Partial Sterility ◽  
X Irradiation ◽  
Paracentric Inversions

Two sex linked paracentric inversions one on m chromosome, marked with bz (bronze body) and the other on M chromosome, marked with w (white eye), were artificially induced with X-irradiation and isolated. The inversions are designated as In. (1)1 and In. (1)2 respectively. The former is more than 23 units long and the later more than 16 units. Both suppress crossing over markedly and are associated with partial sterility. The two inversions are utilized as crossover suppressors in a technique designed for detecting sex linked recessive lethals. The technique works satisfactorily with certain limitations. The possibility of combining inversions with other chromosomal aberrations for genetic control of Aedes aegypti populations is suggested.

scholarly journals Targeting female flight for genetic control of mosquitoes

2020 ◽  
Vol 14 (12) ◽  
pp. e0008876
Author(s):  
David Navarro-Payá ◽  
Ilona Flis ◽  
Michelle A. E. Anderson ◽  
Philippa Hawes ◽  
Ming Li ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Genetic Control ◽  
Fluorescent Protein ◽  
Flight Muscle ◽  
Dominant Negative ◽  
Coding Region ◽  
Protein Marker ◽  
Base Pair Deletion ◽  
Dominant Female ◽  
Males And Females

Aedes aegypti Act4 is a paralog of the Drosophila melanogaster indirect flight muscle actin gene Act88F. Act88F has been shown to be haploinsufficient for flight in both males and females (amorphic mutants are dominant). Whereas Act88F is expressed in indirect flight muscles of both males and females, expression of Act4 is substantially female-specific. We therefore used CRISPR/Cas9 and homology directed repair to examine the phenotype of Act4 mutants in two Culicine mosquitoes, Aedes aegypti and Culex quinquefasciatus. A screen for dominant female-flightless mutants in Cx. quinquefasciatus identified one such mutant associated with a six base pair deletion in the CxAct4 coding region. A similar screen in Ae. aegypti identified no dominant mutants. Disruption of the AeAct4 gene by homology-dependent insertion of a fluorescent protein marker cassette gave a recessive female-flightless phenotype in Ae. aegypti. Reproducing the six-base deletion from Cx. quinquefasciatus in Ae. aegypti using oligo-directed mutagenesis generated dominant female-flightless mutants and identified additional dominant female-flightless mutants with other in-frame insertions or deletions. Our data indicate that loss of function mutations in the AeAct4 gene are recessive but that short in-frame deletions produce dominant-negative versions of the AeAct4 protein that interfere with flight muscle function. This makes Act4 an interesting candidate for genetic control methods, particularly population-suppression gene drives targeting female viability/fertility.

HOMOZYGOUS PERICENTRIC INVERSIONS AND OTHER STUDIES OF INVERSIONS ON CHROMOSOME 3 IN THE MOSQUITO ANOPHELES ALBIMANUS

1981 ◽  
Vol 23 (1) ◽  
pp. 57-64 ◽  
Author(s):  
S. G. Suguna ◽  
J. A. Seawright ◽  
D. J. Joslyn ◽  
M. G. Rabbani
Keyword(s):  
Genetic Control ◽  
Pericentric Inversion ◽  
Chromosome 3 ◽  
Double Heterozygote ◽  
Anopheles Albimanus ◽  
Control Schemes ◽  
Pericentric Inversions ◽  
Partial Sterility ◽  
Radiation Induced ◽  
Experimental Crosses

Two radiation-induced pericentric inversions on chromosome 3 in Anopheles albimanus Wiedemann have been established in homozygous stocks. In(3)17 and In(3)22 cover about 82% and 42%, respectively, of chromosome 3 and should be very useful as crossover suppressors in genetic crosses. In addition, three double-heterozygote stocks were established by combining inversions that had similar breakpoints but were viable only as heterozygotes. These balanced-lethal double heterozygotes can serve as a source of inversion gametes for use in experimental crosses and can be used in surveying populations for lethal chromosomes. Assays of the partial sterility inherent in males heterozygous for a pericentric inversion and a male-linked translocation indicated that the combination of inversions and translocations could be used in genetic control schemes.

scholarly journals Resistance to meiotic drive at the MD locus in an Indian wild population of Aedes aegypti

1977 ◽  
Vol 29 (2) ◽  
pp. 123-132 ◽  
Author(s):  
S. G. Suguna ◽  
R. J. Wood ◽  
C. F. Curtis ◽  
A. Whitelaw ◽  
S. J. Kazmi
Keyword(s):  
Sex Ratio ◽  
Aedes Aegypti ◽  
Genetic Control ◽  
Wild Population ◽  
Sex Ratios ◽  
Meiotic Drive ◽  
In The Wild ◽  
Ratio Distortion ◽  
Sex Ratio Distortion ◽  
Different Parts

SUMMARYFemales from an Indian wild population of Aedes aegypti were crossed to males carrying the sex ratio distorter factor MD which shows meiotic drive. Progenies from F1 males were tested for sex ratio distortion, i.e. the chromosomes from the wild females were screened for their resistance to the action of MD. The distribution of sex ratio in the progenies of different F1 males indicated a polymorphism in the wild population for resistant and sensitive variants of the X chromosome. Seven discrete categories of X appear to exist, associated with sex ratios ranging from 50% ♀ to less than 1·25% ♀. The overall level of resistance varied slightly but significantly in different parts of a town. The results are discussed in relation to the use of sex ratio distortion for genetic control of mosquitoes.

FIELD EXPERIMENTS ON THE COMPETITIVENESS OF MALES CARRYING GENETIC CONTROL SYSTEMS FOR AEDES AEGYPTI

1976 ◽  
Vol 20 (1) ◽  
pp. 8-18 ◽  
Author(s):  
K. K. GROVER ◽  
S. G. SUGUNA ◽  
D. K. UPPAL ◽  
K. R. P. SINGH ◽  
M. A. ANSARI ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Control Systems ◽  
Genetic Control ◽  
Field Experiments
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