Quantification of the histochemical staining for carbonyles and DNA using 3-hydroxy-2-naphthoic acid hydrazide and Fast blue B

1990 ◽  
Vol 94 (5) ◽  
pp. 485-488 ◽  
Author(s):  
G. N�hammer
Keyword(s):  
Acid Hydrazide ◽  
Histochemical Staining ◽  
Fast Blue ◽  
Naphthoic Acid

Molecular structure, vibrational spectroscopic, first-order hyperpolarizability and HOMO, LUMO studies of 3-hydroxy-2-naphthoic acid hydrazide

2010 ◽  
Vol 41 (1) ◽  
pp. 53-62 ◽  
Author(s):  
J. Karpagam ◽  
N. Sundaraganesan ◽  
S. Sebastian ◽  
S. Manoharan ◽  
M. Kurt
Keyword(s):  
Molecular Structure ◽  
Acid Hydrazide ◽  
First Order ◽  
Naphthoic Acid ◽  
Homo Lumo

scholarly journals An improved histochemical method for distinguishing colonic acetylsialomucin from other epithelial mucins.

1981 ◽  
Vol 29 (12) ◽  
pp. 1445-1447 ◽  
Author(s):  
J H Cooper ◽  
R G Durning
Keyword(s):  
Diazonium Salt ◽  
Periodic Acid ◽  
Acid Hydrazide ◽  
Histochemical Method ◽  
Staining Procedure ◽  
Dilute Solution ◽  
Second Stage ◽  
Technical Modification ◽  
Naphthoic Acid ◽  
Dark Blue

A technical modification of the original histochemical methods for distinguishing human colonic acetylsialomucin from other epithelial mucins is described. The modification uses a naphthoic acid hydrazide-diazonium salt sequence in place of the original borohydride or blue Schiff treatments in the first stage of the staining procedure and a more dilute solution of periodic acid in the second stage. By these means, strongly contrasting staining of colonic acetylsialomucins in magenta red and all other mucins in dark blue may be consistently produced.

scholarly journals Histochemical Demonstration of Protein-Bound Alpha-Acylamido Carboxyl Groups

1958 ◽  
Vol 4 (2) ◽  
pp. 169-176 ◽  
Author(s):  
Russell J. Barrnett ◽  
Arnold M. Seligman
Keyword(s):  
Anterior Pituitary ◽  
Acid Hydrazide ◽  
Histochemical Demonstration ◽  
Carboxyl Groups ◽  
Low Molecular Weight ◽  
Elastic Tissue ◽  
Methyl Ketones ◽  
Albino Rat ◽  
Naphthoic Acid ◽  
Specific Proteins

A method has been developed to demonstrate the alpha-acylamido carboxyl groups of protein, taking advantage of the fact that acylamido carboxyl groups are converted to ketonic carbonyls by the action of acetic anhydride and absolute pyridine. The method utilizes deparaffinized sections of tissues fixed in a variety of fixatives. Following the conversion of carboxyls to the methyl ketones, the latter are stained with 2-hydroxy-3-naphthoic acid hydrazide. Control experiments have indicated that methylation of carboxyls prevented staining, as did carbonyl reagents after the carboxyls were transformed to methyl ketones. Leucofuchsin did not stain the ketonic carbonyls, and only elastic tissue stained with 2-hydroxy-3-naphthoic acid hydrazide without the previous use of the catalyzed reaction with anhydride. A brief survey of the reaction on various tissues of the albino rat was made, and the effects of various fixatives were assayed. Of particular interest were certain sites, such as acidophiles of the anterior pituitary gland, where an intense reaction occurred. The possibility exists that certain specific proteins rich in terminal acylamido carboxyl groups, by virtue of their protein side chains or low molecular weight, may be demonstrated by this method.

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