A cytogenetic study directly from chorionic villi of 140 spontaneous abortions

1987 ◽  
Vol 77 (2) ◽  
pp. 137-141 ◽  
Author(s):  
Bernd Eiben ◽  
Sabine Borgmann ◽  
Ingrid Sch�bbe ◽  
Ingo Hansmann
Keyword(s):  
Cytogenetic Study ◽  
Spontaneous Abortions ◽  
Chorionic Villi

Cytogenetic study of spontaneous abortions by transabdominal villus sampling and direct analysis of villi

1999 ◽  
Vol 19 (7) ◽  
pp. 601-603 ◽  
Author(s):  
José María Sánchez ◽  
Lilian Franzi ◽  
Federico Collia ◽  
Silvia L. De Díaz ◽  
Marcelo Panal ◽  
...  
Keyword(s):  
Cytogenetic Study ◽  
Direct Analysis ◽  
Spontaneous Abortions

scholarly journals Relationship between cytogenetic study and ultrasonographic findings in spontaneous abortions conceived by ART and non-ART cycles

2008 ◽  
Vol 90 ◽  
pp. S112
Author(s):  
M. Seki ◽  
S. Furuya ◽  
Y. Fujisawa ◽  
T. Yamaguchi ◽  
H. Tobai ◽  
...  
Keyword(s):  
Cytogenetic Study ◽  
Spontaneous Abortions

scholarly journals Human Decidual Cells Activity in Women with Spontaneous Abortions of Probable CMV Aetiology During the First Trimester of Gestation. An Immunohistochemical Study with CMV-Associated Antigen

2004 ◽  
Vol 47 (3) ◽  
pp. 195-199 ◽  
Author(s):  
Demetrio Tamiolakis ◽  
Ioannis Venizelos ◽  
Maria Lambropoulou ◽  
Athanasia Kotini ◽  
Sophia Barbagadaki ◽  
...  
Keyword(s):  
Stromal Cells ◽  
First Trimester ◽  
Intrauterine Fetal Death ◽  
Spontaneous Abortions ◽  
Endometrial Stromal Cells ◽  
Chorionic Villi ◽  
Decidual Cells ◽  
Lymphoplasmacytic Infiltrate ◽  
Infected Cells ◽  
Mononuclear Infiltrate

Aim: To determine the expression of CMV-associated antigen in the human decidual endometrial stromal cells in spontaneous abortions with no evidence of maternal relapse during the first trimester of gestation. Experimental design: We examined 15 placentas resulting from intrauterine fetal death after spontaneous abortion during the 8th, 10th, and 12th week of gestation respectively, and in which CMV reactivation was ruled out from serological evaluation of the pregnant women at admission, versus equal controls after voluntary abortion following well-documented maternal viral recurrence. In addition, a panel of monoclonal antibodies for the identification of leukocytes (CD45/LCA), B-lymphocytes (CD20/L-26), and T-lymphocytes (CD45RO/UCHL1), was performed. All women received hormonal medication to support gestation, in the cases of spontaneous abortions. Results: Immunohistochemical examination using a specific antibody against cytomegalovirus showed large multinucleated infected cells with intranuclear inclusions, located primarily in the decidual stroma within a lymphoplasmacytic infiltrate in the cases of spontaneous abortions. No evidence of infection was observed in the chorionic villi. In the cases of voluntary abortions same findings were observed in the relevant areas, and a strong evidence of infection was observed in the chorionic villi. Conclusion: This study demonstrates 1) that the decidual endometrial stromal cells can express the CMV-associated antigen prior to serological manifestation of the viral replication, 2) the expression of the antigen is higher in cases of hormonal administration to support gestation. In these cases a mild mononuclear infiltrate of UCHL1 (T marker) positive cells, accompanies the CMV-associated antigen positive cells.

Incidence and Spectrum of Chromosome Abnormalities in Miscarriage Samples: A Retrospective Study of 330 Cases

2019 ◽  
Vol 158 (4) ◽  
pp. 171-183 ◽  
Author(s):  
Cristina Gug ◽  
Adrian Rațiu ◽  
Dan Navolan ◽  
Ioan Drăgan ◽  
Iulia-Maria Groza ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Chorionic Villus ◽  
Normal Karyotype ◽  
Chromosome Abnormalities ◽  
Pcr Analysis ◽  
Chromosomal Anomalies ◽  
Spontaneous Abortions ◽  
Chorionic Villi ◽  
Western Romania

Embryonic chromosome abnormalities are the most important causes of early spontaneous abortions. The aim of this study was to evaluate the spectrum and the frequencies of chromosomal anomalies in spontaneous miscarriages and to correlate these with maternal and gestational age. A retrospective study was conducted based on data obtained from a single medical genetics laboratory that collects cases from Western Romania. Long-term cultures of chorionic villus samples were established for karyotype analysis by GTG banding. Additionally, we performed QF-PCR to detect aneuploidies for chromosomes 13, 18, 21, X, and Y. In total, chorionic villi samples of 330 miscarriages (from August 2007 to November 2018) were analyzed. Results were obtained for 90.6% (299/330) of the cases. The remaining 9.4% (31/330) were excluded from evaluation due to inconclusive results. An abnormal karyotype was found in 156 cases (47.27%), while in 143 cases (43.33%) a normal karyotype was present. Of the abnormal cases, 88 (56.4%) had trisomies, 25 (16.0%) presented polyploidies, 25 (16.0%) had monosomy X, and 19 (11.5%) chromosome rearrangements. QF-PCR analysis identified aneuploidy in 2 out of 8 samples (25%). Cytogenetic investigations of spontaneous abortions provide valid data as to the cause of the abortion. This information may also be helpful for genetic counseling and considering future pregnancies.

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