Aneuploidy in first trimester chorionic villi and spontaneous abortions: Windows into the origin and fate of aneuploidy through embryonic and fetal development

2020 ◽  
Author(s):  
Peter Benn ◽  
Francesca Romana Grati
Keyword(s):  
Fetal Development ◽  
First Trimester ◽  
Spontaneous Abortions ◽  
Chorionic Villi ◽  
Embryonic And Fetal Development

scholarly journals Human Decidual Cells Activity in Women with Spontaneous Abortions of Probable CMV Aetiology During the First Trimester of Gestation. An Immunohistochemical Study with CMV-Associated Antigen

2004 ◽  
Vol 47 (3) ◽  
pp. 195-199 ◽  
Author(s):  
Demetrio Tamiolakis ◽  
Ioannis Venizelos ◽  
Maria Lambropoulou ◽  
Athanasia Kotini ◽  
Sophia Barbagadaki ◽  
...  
Keyword(s):  
Stromal Cells ◽  
First Trimester ◽  
Intrauterine Fetal Death ◽  
Spontaneous Abortions ◽  
Endometrial Stromal Cells ◽  
Chorionic Villi ◽  
Decidual Cells ◽  
Lymphoplasmacytic Infiltrate ◽  
Infected Cells ◽  
Mononuclear Infiltrate

Aim: To determine the expression of CMV-associated antigen in the human decidual endometrial stromal cells in spontaneous abortions with no evidence of maternal relapse during the first trimester of gestation. Experimental design: We examined 15 placentas resulting from intrauterine fetal death after spontaneous abortion during the 8th, 10th, and 12th week of gestation respectively, and in which CMV reactivation was ruled out from serological evaluation of the pregnant women at admission, versus equal controls after voluntary abortion following well-documented maternal viral recurrence. In addition, a panel of monoclonal antibodies for the identification of leukocytes (CD45/LCA), B-lymphocytes (CD20/L-26), and T-lymphocytes (CD45RO/UCHL1), was performed. All women received hormonal medication to support gestation, in the cases of spontaneous abortions. Results: Immunohistochemical examination using a specific antibody against cytomegalovirus showed large multinucleated infected cells with intranuclear inclusions, located primarily in the decidual stroma within a lymphoplasmacytic infiltrate in the cases of spontaneous abortions. No evidence of infection was observed in the chorionic villi. In the cases of voluntary abortions same findings were observed in the relevant areas, and a strong evidence of infection was observed in the chorionic villi. Conclusion: This study demonstrates 1) that the decidual endometrial stromal cells can express the CMV-associated antigen prior to serological manifestation of the viral replication, 2) the expression of the antigen is higher in cases of hormonal administration to support gestation. In these cases a mild mononuclear infiltrate of UCHL1 (T marker) positive cells, accompanies the CMV-associated antigen positive cells.

scholarly journals HDlive Study of Fetal Development and Behavior

2014 ◽  
Vol 8 (3) ◽  
pp. 250-265 ◽  
Author(s):  
Hirokazu Tanaka ◽  
Toshiyuki Hata ◽  
Rina Uematsu
Keyword(s):  
Facial Expressions ◽  
Fetal Development ◽  
Obstet Gynecol ◽  
Fetal Brain ◽  
First Trimester ◽  
Future Research ◽  
Facial Movements ◽  
Embryonic And Fetal Development ◽  
First Trimester Of Pregnancy ◽  
And Behavior

ABSTRACT We present the latest HDlive studies of fetal development and behavior during pregnancy. Marked changes of embryonic and fetal development and movement with advancing gestation were noted in the first trimester of pregnancy. Various realistic facial expressions (or facial movements) were recognized in the second and third trimesters. In particular, blinking, mouthing, swallowing, yawning, tongue expulsion, and sucking were clearly depicted. Moreover, various extraordinarily realistic features of fetal emotion-like behavior were identified. HDlive observation of fetal behavior and facial expressions may reflect the normal and abnormal neurological development of the fetus. HDlive may become an important modality in future research on fetal neurobehavioral development and assist in evaluation of the fetal brain function. How to cite this article Hata T, Kanenishi K, Hanaoka U, Uematsu R, Marumo G, Tanaka H. HDlive Study of Fetal Development and Behavior. Donald School J Ultrasound Obstet Gynecol 2014;8(3):250-265.

scholarly journals A single-cell survey of the human first-trimester placenta and decidua

2018 ◽  
Vol 4 (10) ◽  
pp. eaau4788 ◽  
Author(s):  
Hemant Suryawanshi ◽  
Pavel Morozov ◽  
Alexander Straus ◽  
Nicole Sahasrabudhe ◽  
Klaas E. A. Max ◽  
...  
Keyword(s):  
Single Cell ◽  
Fetal Development ◽  
Cell Types ◽  
First Trimester ◽  
Bioinformatic Analysis ◽  
Cell Type ◽  
Early Gestation ◽  
Embryonic And Fetal Development ◽  
Single Cell Rna Sequencing ◽  
Cell Type Specific

The placenta and decidua interact dynamically to enable embryonic and fetal development. Here, we report single-cell RNA sequencing of 14,341 and 6754 cells from first-trimester human placental villous and decidual tissues, respectively. Bioinformatic analysis identified major cell types, many known and some subtypes previously unknown in placental villi and decidual context. Further detailed analysis revealed proliferating subpopulations, enrichment of cell type–specific transcription factors, and putative intercellular communication in the fetomaternal microenvironment. This study provides a blueprint to further the understanding of the roles of these cells in the placenta and decidua for maintenance of early gestation as well as pathogenesis in pregnancy-related disorders.

scholarly journals Downregulation of A1 and A2B adenosine receptors in human trisomy 21 mesenchymal cells from first-trimester chorionic villi

2012 ◽  
Vol 1822 (11) ◽  
pp. 1660-1670 ◽  
Author(s):  
Stefania Gessi ◽  
Stefania Merighi ◽  
Angela Stefanelli ◽  
Prisco Mirandola ◽  
Alessandra Bonfatti ◽  
...  
Keyword(s):  
Adenosine Receptors ◽  
Trisomy 21 ◽  
First Trimester ◽  
Mesenchymal Cells ◽  
Chorionic Villi

TSH/miR‐17‐5p/ZNF367 axis is related to spontaneous abortion in patients with TSH above 2.5 mIU/L

2021 ◽  
Author(s):  
Yang Yang ◽  
Jiashu Li ◽  
Yingying Zhou ◽  
Wen Dai ◽  
Weiping Teng ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Spontaneous Abortion ◽  
Biological Function ◽  
Zinc Finger Protein ◽  
First Trimester ◽  
Cell Counting ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Chorionic Villi ◽  
Increased Risk

Elevated thyroid stimulating hormone (TSH) is associated with an increased risk of spontaneous abortion (SA); however, the associated mechanism remains unclear. This study aimed to investigate the expression of microRNAs (miRNAs) and pathogenesis in the chorionic villi of TSH > 2.5 mIU/L-related SA patients. The chorionic villi were collected from pregnant women in the first trimester with TSH > 2.5 mIU/L with or without SA, as well as TSH < 2.5 mIU/L with or without SA to determine the level of miRNA expression. Differentially expressed miRNAs were confirmed by qRT-PCR in a total of 92 subjects. Cell counting kit-8 (CCK8), wound healing, transwell assays, and Western blotting were used to measure cellular biological functions and related protein in HTR-8/SVneo cells. The potential mechanisms were determined using a Luciferase reporter assay and rescue experiment. Compared with normal pregnant women, miR-17-5p was decreased and zinc finger protein 367 (ZNF367) was upregulated in the chorionic villi of TSH > 2.5 mIU/L-related SA patients. Using HTR-8/SVneo cells, we demonstrated that elevated TSH inhibited miR-17-5p expression, as well as trophoblast migration and invasion. The overexpression of miR-17-5p targeted and inhibited ZNF367 expression promoting the biological function of trophoblasts. Further studies confirmed that ZNF367 interference partially reversed the biological function of the miR-17-5p inhibitor on HTR-8/SVneo cells. Taken together, our results showed that miR-17-5p promoted biological function of trophoblasts by suppressing ZNF367.

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