Genetically controlled anthocyanin synthesis in cell cultures of Matthiola incana

1982 ◽  
Vol 1 (3) ◽  
pp. 98-100 ◽  
Author(s):  
Birgit Leweke ◽  
Gert Forkmann
Keyword(s):  
Cell Cultures ◽  
Anthocyanin Synthesis ◽  
Matthiola Incana

Stimulation of anthocyanin synthesis in grape (Vitis vinifera) cell cultures by pulsed electric fields and ethephon

2011 ◽  
Vol 108 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Nay Min Min Thaw Saw ◽  
Heidi Riedel ◽  
Zhenzhen Cai ◽  
Onur Kütük ◽  
Iryna Smetanska
Keyword(s):  
Vitis Vinifera ◽  
Electric Fields ◽  
Cell Cultures ◽  
Pulsed Electric Fields ◽  
Anthocyanin Synthesis ◽  
Stimulation Of

UDP-Glucose: Anthocyanidin/FIavonol 3-O-Glucosyltransferase in Enzyme Preparation from Flower Extracts of Genetically Defined Lines of Matthiola incana R. Br.

1986 ◽  
Vol 41 (7-8) ◽  
pp. 699-706 ◽  
Author(s):  
M. Teusch ◽  
G. Forkmann ◽  
W. Seyffert
Keyword(s):  
Enzyme Activity ◽  
Structural Gene ◽  
Enzyme Preparation ◽  
Reaction Rate ◽  
Flower Colour ◽  
Hydroxyl Group ◽  
Anthocyanin Synthesis ◽  
Ph Optimum ◽  
Matthiola Incana ◽  
Highly Active

Abstract In flower extracts of Matthiola incana an enzyme catalyzing the transfer of glucose from UDP- glucose to the hydroxyl group at 3-position of anthocyanidins and flavonols was demonstrated. The pH-optimum of this reaction is at pH 8.5 for pelargonidin and pH 9.5 for quercetin as substrate. The reaction is inhibited by both substrates above 10 nmol per assay. The enzyme is highly active, within 30 sec 3 nmol of 3-glucosides were formed. At 30 °C the enzyme is stable for hours and at -20 °C months. Besides UDP-glucose, TDP-glucose is a suitable glucosyl-donor, but with a reduced (70%) reaction rate. Enzyme activity is clearly inhibited by Fe2+ and Cu2+ ions, and by diethylpyrocarbonate. Acyanic or pale coloured mutants of several genes interfering with anthocyanin synthesis after dihydroflavonol formation show a more or less drastically reduced enzyme activity (5-40%). But none of these genes can be regarded as the structural gene for the 3-glucosyltransferase. The influence of these genes on enzyme activity and flower colour is dis­cussed.

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

Bacteriophages Associated with Turkey Coecums in Bluecomb-Infected and Healthy Birds

1969 ◽  
Vol 27 ◽  
pp. 226-227
Author(s):  
A. E. Ritchie
Keyword(s):  
Host Cell ◽  
Causal Relationship ◽  
Cell Cultures ◽  
Cell Interaction ◽  
Etiologic Agent ◽  
Viral Origin ◽  
Intestinal Contents ◽  
Host Cell Interaction

The cause of bluecomb disease in turkeys is unknown. Filtration of infective intestinal contents suggests a viral origin. To date, it has not been possible to isolate the etiologic agent in various cell cultures. The purpose of this work was to characterize as many virus-like entities as were recognizable in intestines of both healthy and bluecomb-infected turkeys. By a comparison of the viral populations it was hoped that some insight might be gained into the cause of this disease. Studies of turkey hemorraghic enteritis by Gross and Moore (Avian Dis. 11: 296-307, 1967) have suggested that a bacteriophage-host cell interaction may bear some causal relationship to that disease.

The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

1973 ◽  
Vol 31 ◽  
pp. 552-553
Author(s):  
T. M. Crisp ◽  
F.R. Denys
Keyword(s):  
Fine Structure ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Single Injection ◽  
Surrounding Medium ◽  
Petri Dish ◽  
Female Rats ◽  
Vaginal Smear ◽  
Immature Female ◽  
Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

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